Model EZ1 - Advanced XL Performs Automated Nucleic Acid Purification
From Nucleic Acid Purification
The EZ1 Advanced XL performs automated nucleic acid purification for a wide range of sample types relevant for molecular diagnostics, human identity testing, forensics, biomedical research, and gene expression analysis.
Product Details
Instrument and service agreement package: robotic workstation for automated purification of nucleic acids from up to fourteen samples using EZ1 Kits; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits.
- 24/7 automatic processing of online orders
- Knowledgeable and professional Product & Technical Support
- Fast and reliable (re)-ordering
Features
- High-quality genomic DNA and RNA from a range of sample materials
- Viral RNA/DNA and bacterial DNA from biomedical samples
- Throughput of up to 14 samples per run
- Effortless data management with full traceability
- UV lamp to help minimize contamination risk
Performance
Standardization and traceability
The EZ1 Advanced XL sets new standards in data management. Barcode reading enables complete tracking of samples and reagents throughout the entire purification process. Notes and assay identification information can also be easily entered. For increased process control and reliability, reagent identification and lot numbers are logged together with the expiration date, and a warning is issued if the expiration date has been exceeded. The run report, automatically generated at the end of each protocol, contains all relevant instrument information, including maintenance procedures and decontamination data. The instruments generate a pdf and a csv file after each run. Data can be easily transferred to a printer or computer within the network and the report file can be subsequently processed by a LIMS (Laboratory Information Management System).
Proven technology with improved performance
Proven EZ1 Kits, in combination with EZ1 Advanced XL Cards, ensure high-performance results in purification procedures. The unrivaled ease of use of the EZ1 Advanced XL enables fast and reproducible purification, without the need for specialized training or previous experience with laboratory automation. The extensive application range, including DNA purification from blood, tissues, and human identity and forensic samples, RNA purification from cells and tissues, and purification of viral nucleic acids from a range of sample types, delivers the flexibility that is essential for laboratories.
Safety and reliability
The EZ1 Advanced XL has been designed to ensure high process safety, high performance, and user convenience. A sensor ensures that the instrument door remains closed during sample preparation, reducing contact of laboratory personnel with potentially infectious samples and protecting sample integrity. At the end of each run, an intelligent-design UV lamp decontaminates the inner surface of the instrument. UV decontamination is highly effective in eliminating Gram-positive and Gram-negative bacteria (see tables). If required, the instrument can be placed in a safety cabinet, providing increased user safety.
E. coli colony numbers
Six replicates (20 μl each) of Escherichia coli cultures (17,750 CFU/ml) were irradiated using the integrated UV lamp of the EZ1 Advanced, followed by growth on plates in appropriate culture medium. Three independent irradiation runs were performed. Irradiation using the UV lamp was highly effective in eliminating bacteria.
S. haemolyticus colony numbers
Six replicates (20 μl each) of Staphylococcus haemolyticus cultures (15,700 CFU/ml) were irradiated using the integrated UV lamp of the EZ1 Advanced, followed by growth on plates in appropriate culture medium. Three independent irradiation runs were performed. Irradiation using the UV lamp was highly effective in eliminating bacteria.
Principle
Automated sample preparation enables efficient, standardized purification of nucleic acids. Manual sample handling is minimized, ensuring safety to the user and reliable processing of samples.
The EZ1 Advanced XL instrument uses proven EZ1 Kits for purification of DNA, RNA, and viral nucleic acids from a wide range of starting materials. Purified nucleic acids are ready for immediate use in downstream applications. The combination of an easy-to-use instrument, with error-free protocol selection and worktable setup, and prefilled, sealed reagent cartridges makes nucleic acid purification easy.
Procedure
Flexibility and ease of use
The self-contained EZ1 Advanced XL instrument ensures optimal ease of use and walkaway automation. A wide range of protocols is available for purification of DNA, RNA, or viral nucleic acids. Up to 14 samples can be processed per run in as little as 20 minutes. The instrument is operated via a keypad, and instrument information is displayed on the VFD (vacuum fluorescent display).
All processing steps are performed by the instruments — from piercing reagent cartridges to elution of pure nucleic acids. A separate computer is not required for operation, and EZ1 Kits include all reagents and accessories required to process your samples. EZ1 Kits provide prefilled, foil-sealed reagent cartridges that remain sealed until the instrument door is closed and the protocol run started, reducing the risk of contamination during setup.
If desired, up to 4 EZ1 Advanced XL instruments can be connected to an external computer, expanding throughput up to 56 samples per run. This enables you to easily adapt your laboratory setup to changing sample preparation requirements.
EZ1 Kits and EZ1 Advanced XL Cards for a wide range of applications
The EZ1 Advanced XL uses proven EZ1 Kits with preprogrammed protocols on EZ1 Advanced XL Cards. EZ1 Kits are available for a broad range of applications including work in human identity and forensics, biomedical research, and gene expression analysis (see table).
Applications
Nucleic acids purified using the EZ1 Advanced XL are ready for use in a wide range of downstream applications, including:
- Oncology, forensics, and genetic identity testing
- Genotyping
- Infectious disease research, including bacterial genotyping
- Gene expression analysis
Supporting Data and Figures
Simple protocol.
1. Insert the EZ1 Advanced XL Card with the desired protocol into the card slot and start the instrument. 2. Place samples and prefilled, sealed reagent cartridges into the instrument. Samples are processed automatically and purified nucleic acids are transferred to elution tubes. 3. Remove pure nucleic acids in 1.5 ml microcentrifuge tubes at a concentration suited to the downstream application.
Specifications
- Features: Touch LCD user interface, IC card with protocols/software, barcode reader, UV lamp
- Pipetting range: 50-1000 µl
- Kits designed for this instrument: QIAGEN EZ1 Kits
- Technology: Magnetic-particle technology
- Instrument dimensions: 510 x 510 x 570 mm (w x d x h)
- Technical data of the instrument: Weight 48 kg, 100–240 V AC, 50–60 Hz
- Protocols/main application on this instrument: Purification of DNA, mRNA, total RNA, and viral RNA and DNA
- Samples per run (throughput): 1–14 samples per run
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