NECi Superior Enzymes

- Model NTK-MPSR - Standard Range Microplate Assay Nitrate Test Kit


Microplate Nitrate Testing For Your Lab; Accurate, Economical, Safe, and Environmentally Benign Nitrate Analysis. Use this kit for quantification of nitrate in essentially any aqueous sample. Reactions occur on a 96-well microplate provided with the kit. This test kit is based on the enzyme Nitrate Reductase (NaR), catalyzing the reduction of nitrate to nitrite using the natural electron donor NADH. The nitrite reacts with color reagents under acidic conditions to produce a visible color. The concentration of nitrate in the original sample is determined by measuring absorbance with a microplate reader using a 540 nm filter, versus nitrate concentration in nitrate standards. Choose from: Standard Range (1.0 ppm - 10.0 ppm Nitrate-N) or Low Range (0.5 ppm - 1.0 ppm Nitrate-N) Freshwater or Saltwater Analysis

You will need variable pipetters, a 25mL graduated cylinder, a vortex-type mixer, a microplate mixer, a microplate reader or photometer for reading Absorbance at 540 nm± 20 nm, clean and nitrate-free test tubes, a timer, reagent grade water, and concentrated HCl.

Each Kit Contains:

  • (1) 15mL Amber Bottle Assay Buffer (liquid)
  • (1) 15mL Amber Bottle Color Reagent No. 1 (solid)
  • (1) 15mL Amber Bottle Color Reagent No. 2 (solid)
  • (1) Tube NADH (freeze-dried)
  • (1) Tube Nitrate Reductase (freeze-dried)
  • (1) Squeeze-bulb Enzyme Diluent
  • (1) 1.5mL Tube 100 ppm Nitrate-N Nitrate Standard
  • (1) 96-well Microplate
  • (2) Pipetter Reservoir Basins
  • (6) Microcentrifuge Tubes
  • (1) Set of Instructions
  • (1) 20mL Tube Salt Water (only if you are testing seawater, please specify when ordering)

For more detailed information about this kit, please refer to the instruction sheet.

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Call (1-888-NITRATE or 1-888-648-7283) for bulk and repeat order discount prices.

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Questions? Call 1-888-NITRATE or 1-888-648-7283

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