QIAGEN

- Virus Spin Kit

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QIAamp MinElute Virus Kits simplify purification of viral DNA and RNA with fast spin-column or vacuum procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes up to 0.2 ml, and the QIAamp MinElute Virus Vacuum Kit can be used with starting sample volumes up to 0.5 ml. Both kits combine the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. Using the QIAamp MinElute Virus Spin Kit, the purification process can be fully automated on the QIAcube.

For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids using spin processing

  • Rapid purification of high-quality viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

Product Details

  • Format and processing: QIAamp MinElute columns processed by centrifugation
  • Sample sources: Fresh or frozen plasma, serum, and other cell-free body fluids
  • Sample size: 200 µl
  • Preparation time: <1 hour
  • Elution volume: 20–150 µl

The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA.

The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.

The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane. Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT/PCR (see figures 'High Sensitivity in PCR and RT-PCR Using QIAamp MinElute Virus Kits - A' and 'High Sensitivity in PCR and RT-PCR Using QIAamp MinElute Virus Kits - B').

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