QIAxcel - Effortless PCR Fragment And RNA Analysis System
From Nucleic Acid Detection and Analysis
The QIAxcel system is a multicapillary electrophoresis system designed to overcome the bottlenecks of gel electrophoresis. The fully automated system can process up to 96 samples per run. The optimized system, including gel cartridge and BioCalculator Software, delivers reliable results in a wide range of applications, and provides unrivaled resolution, speed, and throughput in DNA and RNA analyses. A wide range of markers and other accessories is available. For PCR success at first attempt, use QIAGEN PCR kits and enzymes and benefit from our winning combination for fast and highly specific PCR and fragment detection.
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For effortless, automated analysis of DNA fragment...
For effortless, automated analysis of DNA fragments and RNA
- Safety and convenience with ready-to-use gel cartridges
- Streamlined workflow minimizes analysis time
- Robust results for nucleic acid concentrations as low as 0.1 ng/µl
- Accurate analyses with resolution down to 3–5 bp
- Standardized processing with digital data collection
Principle
The QIAxcel system consists of the QIAxcel analyzer, a novel multiplexed fluorescence detection design including an array of light-emitting diodes and micro-optical collectors, QIAxcel Gel Cartridges, BioCalculator Software, and a computer. Ready-to-run gel cartridges contain 12 separation micro-channels with a built-in gel matrix for fast, high-resolution analysis of up to 96 samples in parallel.
Hands-free sample loading and self-contained components minimize exposure to hazardous chemicals such as ethidium bromide. The high detection sensitivity provided by the QIAxcel analyzer enables robust results even with low concentrations of nucleic acid. The QIAxcel system speeds up DNA and RNA analysis applications by eliminating slab-gel analysis while streamlining entire sample purification and analysis workflow.
Procedure
The QIAxcel system is operated with a few, simple steps: the gel cartridge, the filled buffer tray, and samples in 96-well plates or in PCR tubes or strips are loaded, and the separation method is selected. Tedious gel preparation and extensive user training is eliminated, streamlining the workflow and facilitating integration of the system in daily routines. Within minutes of starting a run, the first results appear in real time on the computer screen. With a resolution of 3-5 bp for fragments smaller than 0.5 kb, the QIAxcel analyzer ensures greater accuracy than slab-gel methods as well as greater confidence in data interpretation. Sample consumption is less than 0.1 µl per analysis, saving your precious sample for further downstream analysis.
Convenient Data Analysis And Storage
The BioCalculator Software, provided with the QIAxcel system, is a powerful and user-friendly tool designed to support data collection and analysis. Data can be viewed as both electropherogram and gel image. Interactive tools simplify analysis and facilitate quick data interpretation. Results can be viewed individually or overlay views can be created for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation and a unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet your documentation needs.
Application/Industries Served
The QIAxcel system offers a broad range of optimized applications and is highly suited for research in academic as well as pharmaceutical, biotechnology, and biomedical laboratories. Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids, including single or multiple PCR fragments, DNA digested with restriction endonucleases, synthesized oligonucleotides, total RNA, and cRNA.
Specification
- Instrument dimensions: 362 x 559 x 381 mm (w x d x h) (Height if door open: 529 mm)
- Kits designed for this instrument: QIAxcel DNA Kits (High Resolution Kit, Screening Kit, and DNA Fast Analysis Kit); QIAxcel RNA QC Kit v2.0
- Protocols/main application on this instrument: DNA fragment and RNA analysis
- Technology: Capillary electrophoresis
- Samples per run; throughput: Up to 96 samples per run
- Features: Nucleic acid concentrations as low as 0.1 ng/µl; Resolution down to 3-5 bp
- Mass: 23 kg
- Power: 100-240 V AC, 50-60 Hz, 360 VA
- Operating temperature: 20-28°C
- Humidity: 10-75% (non-condensing)
- Altitude: Up to 2000 m
- Place of operation: For indoor use only
- Storage temperature: 15-30°C in manufacturer's package
- Pollution level: 2
- Overvoltage category: II
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