QuantiTect - Model Virus Kits - Highly Sensitive Detection Of Pathogen Nucleic Acids (DNA and RNA) In Single- Or Multiplex Assays
The QuantiTect Virus Kit is specially designed for highly sensitive detection of viral nucleic acids. Multiplex assays enable detection of multiple viral RNA and/or DNA targets plus internal controls without loss of sensitivity. The supplied 5x master mix contains HotStarTaq Plus DNA Polymerase and dNTP mix in addition to ROX dye, at a concentration optimized for real-time cyclers that require a high ROX concentration in the amplification reaction. The supplied QuantiTect Virus RT Mix contains a unique formulation of Sensiscript Reverse Transcriptase optimized for highly sensitive detection of viral RNA. The QuantiTect Virus +ROX Vial Kit is similar to the QuantiTect Virus Kit except that it includes a ROX-free 5x master mix and a separate vial of ROX dye.
For highly sensitive detection of viral RNA and/or DNA
- High sensitivity in single and multiplex assays
- Detection of viral RNA and/or DNA in the same reaction
- Clear detection of weak positive signals
- Fast universal 2-step protocols
- 5x master mix for higher sensitivity with more sample input
QuantiTect Virus Kits provide highly sensitive detection of viral nucleic acids in single or multiplex assays. Multiplex assays enable detection of multiple viral RNA and/or DNA targets plus internal controls over a wide linear range without loss of sensitivity.
Amplification using QuantiTect Virus Kits provides steep sigmoidal curves over a range of dilutions, even for low template amounts with high CT values. This enables accurate CT value determination for quantification of viral nucleic acids in real-time PCR.
QuantiTect Nucleic Acid Dilution Buffer supplied with the kits stabilizes RNA and DNA standards during dilution and reaction setup and prevents loss of nucleic acids on plastic surfaces, such as tubes or pipet tips.
The buffer enables reliable dilution of standards used to quantify viral nucleic acids, giving a wide linear range, from low to high CT values. The buffer ensures longer storage of standards without degradation.
QuantiTect Virus Kits provide highly sensitive real-time single or multiplex PCR or one-step RT-PCR using sequence-specific probes for detection of viral DNA and/or RNA plus internal controls. The kits can be used on a wide range of real-time thermal cyclers.
Multiplex PCR and RT-PCR Protocols Using QuantiTect Virus Kits on Different Real-Time Thermal Cycler
For fast and highly sensitive end-point one-step RT-PCR applications, including virus detection, we recommend using the QIAGEN OneStep RT-PCR Kit.