REAGEN - Model RNA97008 - Okadaic Acid (DSP) ELISA Test Kit
The unique features of the kit are:
- High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods
- High sensitivity (0.3 ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples).
- High reproducibility.
- A quick ELISA assay(less than 1.5 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The second antibody against AntiOkadaic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic AcidHRP Conjugate) and AntiOkadaic Acid Antibody. If the okadaic acid residue is present in the sample, it will compete with Okadaic AcidHRP Conjugate for the AntiOkadaic Acid Antibody, thereby preventing the Okadaic AcidHRP Conjugate from binding to the AntiOkadaic Acid Antibody. The AntiOkadaic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic acidresidue concentration in the sample.