Reagen LLC

Reagen LLC

- Model RNA97008 - Okadaic Acid (DSP) ELISA Test Kit

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Contact Angela : reagenllc@gmail.com Product Description REAGEN™ Okadaic acid elisa kit is a competitive enzyme immunoassay for the quantitative analysis of Okadaic Acid (DSP) in mussels and water samples. The unique features of the kit are: • High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods • High sensitivity (0.3 ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples) • High reproducibility • A quick ELISA assay (less than 1.5 hours regardless of number of samples). Sample Type Detection Limit (ng/g or ppb) Mussels 30 Water 6 Spec:96T Shelf life:12 months

The unique features of the kit are:

  • High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods
  • High sensitivity (0.3 ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples).
  • High reproducibility.
  • A quick ELISA assay(less than 1.5 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The second antibody against AntiOkadaic Acid  Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic AcidHRP Conjugate) and AntiOkadaic Acid Antibody. If the okadaic acid  residue is present in the sample, it will compete with Okadaic AcidHRP Conjugate for the AntiOkadaic Acid Antibody,  thereby preventing the Okadaic AcidHRP Conjugate from binding to the AntiOkadaic Acid Antibody. The AntiOkadaic  Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the  color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic  acidresidue concentration in the sample.

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