REAGEN LLC

- Model RNA97010 - Histamine Enzymatic Assay Kit

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Histamine Enzymatic Assay Kit RNA97010 Product Description REAGEN™ Histamine Enzymatic Assay Kit is a Colorimetric Enzymatic Assay for the Determination of histamine in Fresh fish / Seafood, fish Meal, Wine and Milk. The unique features of the kit are: • Rapid, cost-effective aqueous extraction method with high recovery • New methanol-free extraction protocol • High sensitivity and low detection limit (6 ppm for fresh fish / seafood) • High reproducibility • A very rapid (10 minutes) and robust enzyme-based assay which does not require chemical derivatization or expensive instrumentation Sample Type Detection Limit (ng / g or ppb) Meat / Seafood 6 Fish Meal 10 Milk 40 Wine 40 Spec:96T Shelf life:12 months

The unique features of the kit are:

  • Rapid, cost-effective aqueous extraction method with high recovery.
  • New methanol-free extraction protocol
  • High sensitivity and low detection limit (6 ppm for fresh fish/seafood).
  • High reproducibility.

A very rapid (10 minutes) and robust enzyme-based assay which does not require chemical derivatization or expensive instrumentation.

Procedure Overview

The REAGENHistamine Enzymatic Assay Kit measures the chemical reduction of a dye molecule using a histamine-specific enzyme. The color change caused by reduction of the dye (measured by the absorbance at 450 nm) is related to the amount of histamine present in the sample.

Sensitivity

Sample Type - Detection Limit (ng/g or ppb)
Meat/Seafood- 6
Fish Meal- 10
Milk- 40
Wine- 40

Specificity

Analytes -Cross-Reactivity (%)
Histamine- 100

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