Reagen LLC

Reagen LLC

- Model RNP96017 - Trifluralin ELISA Test Kit


Contact Angela Product Description REAGEN™ Trifluralin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of trifluralinl in fish, shrimp, meat and water. The unique features of the kit are: High recovery (75-115%), rapid (10 - 40 minutes), and cost-effective extraction methods . High sensitivity (0.25 ng/g or ppb). Quick ELISA assay (less than 2 hours regardless of the number of samples). High reproducibility. Sample Type Detection Limit (ng/g or ppb) Fish/Meat 0.4 Shrimp 0.3 water 0.4 Spec:96 T Shelf life:24 months

The unique features of the kit are:

  • Rapid (10 minutes), and organic reagent-free extraction method with high recovery (80 - 105%).
  • High sensitivity (0.5 ng/g or ppb) and low detection limit (1 ng/g or ppb for honey).
  • High reproducibility.
  • A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

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