Shimadzu Scientific Instruments Inc

- Model SIL-20A/20AC - Prominence HPLC Autosamplers


Combining the smaller footprint of the SIL-10ADvp with the rack style and speed of the SIL-HT, the SIL-20A/20AC Autosampler provides excellent analysis efficiency through improvements in sample injection speed, accuracy, and processing capacity. Revolutionary cross-contamination countermeasures make it possible to perform analysis without carryover, even with high-sensitivity LC-MS/MS detection.

SIL-20A - High-Throughput Analysis
A total-volume injection type autosampler, the SIL-20A enables high-speed injection and high-throughput sample processing without wasting sample. It is designed to ensure greater stability, with improved durability attained through modifications in valves and sample loops. Multiple vial and plate types are supported, and a control vial rack isolates standards from analytical samples.

SIL-20AC - Efficient Sample Cooling
The SIL-20AC is equipped with a sample cooler with a built-in dehumidifier to minimize condensation problems. Samples can be maintained at a fixed temperature from 4ºC to 40ºC. Temperature equilibration is rapid and uniform, so heat or cold sensitive sample constituents remain stable.


Near-Zero Carryover
(As demonstrated on the Applied Biosystems/MDS Sciex API 5000TM system)

Adsorption of sample constituents has been reduced to an absolute minimum. Enhancements include:

  • Special needle treatment (patent pending) - masks active sites and minimizes ionic interactions
  • One piece injection port/needle seal at valve center - eliminates sample transfer tubing
  • Redesigned needle seal to minimize surface area
  • Low-adsorption valve materials

These advancements combine to eliminate cross contamination - even with difficult basic and hydrophobic compounds. For especially difficult samples, dual-solvent rinsing is available.

High Speed/Throughput - 10s for a 10 µL injection
Ultra-high-speed sample processing - 10 seconds for a 10 µL injection - is now possible with the SIL-20A/20AC. Used together with a high-speed separation column makes an analysis cycle time of less than one minute a reality.

Precise Sample Injection
Greater injection volume accuracy has been attained by incorporating a high-performance precision metering pump. Injection volumes of less than 1 µL are possible, and the direct injection method means valuable samples are never wasted.

Optional Rack Changer
Significantly Increases Sample Capacity

With increased speed, capacity becomes a limiting factor for unattended operation. The optional rack changer automatically loads microplates into the SIL-20A/AC sample compartment. The 12-plate capacity ensures convenient processing of over 1000 samples. The 'C' model changer has the same plate capacity and incorporates a 4ºC to 40ºC cooling function, matching that of the SIL-20AC.

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