TaqMan - Model SARS-CoV-2 - Mutation Panel
The Applied Biosystems TaqMan SARS-CoV-2 Mutation Panel comes with a customizable menu of 22 verified real-time PCR assays to give you answers you need today, while preparing you for what’s to come. This scalable solution lets you run a few or hundreds of samples to identify one or many mutations-all on your current real-time PCR instrumentation.
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Features of the TaqMan SARS-CoV-2 Mutation Panel include:
- Customizable-choose from a menu of 22 verified real-time PCR assays that allows you to identify currently relevant SARS-CoV-2 mutations and adapt quickly as additional mutations and variants emerge
- Convenient-use your current real-time PCR instrumentation to conduct reflex of SARS-CoV-2 samples
- Scalable-run a few or hundreds of samples to identify for one or many mutations
- Unique, streamlined workflow-combines our gold-standard TaqMan SNP Genotyping Assays with a 1-step RT-PCR reaction; go from RNA to results in just over 1 hour
Product Details
- Targets: Choose from a menu of 22 verified real-time PCR SNP assays
- Assay design
- Sequence-specific forward and reverse primers to amplify the target sequence region. The reverse primer also primes reverse transcription of the SARS-CoV-2 genomic RNA sequences. Each assay includes two TaqMan minor groove binder (MGB) probes with nonfluorescent quenchers (NFQ):
- One VIC dye labeled probe to detect the reference sequence
- One FAM dye labeled probe to detect the mutation sequence
- Available sizes
- 300 reactions
- 1,000 reactions
- Sample input: RNA extracted from SARS-CoV-2 samples with a CT value of less or equal to 30
- Turn around time: 1 hour and 10 min from extracted RNA to results
- Recommended instruments: Any real-time PCR instrument such as the Applied Biosystems 7500, 7500 Fast, QuantStudio 5, and QuantStudio 7 systems
- Recommended software: QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module
Performance
Our menu of verified real-time PCR assays provide robust detection of SARS-CoV-2 mutations. Below are results (cluster plots) as viewed using QuantStudio Design and Analysis Software 2.5 with the Genotyping Analysis Module. These cluster plots show very clear discrimination between the wild type samples (red dots along the x-axis) and the mutation samples (blue dots along the y-axis).
Figure 2. delH69V70 (also known as 69-70del) is a ...
Figure 2. delH69V70 (also known as 69-70del) is a known mutation of B.1.1.7 (501Y.V1 or UK variant). delH69V70 is not exclusive to B.1.1.7.
- Wild type detected
- delH68V70 mutation detected
Figure 3. N501Y is commonly found in B.1.1.7 (UK v...
Figure 3. N501Y is commonly found in B.1.1.7 (UK variant), B.1.351 (South African variant), P.1 (Brazilian variant).
- Wild type detected
- N501Y mutation detected
Analysis software
Use QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module.
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