Chelsea Technologies Group

- Low Cost Digital Fluorometers

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The innovative TriLux range of digital, in-situ, multi-wavelength, fluorimeters provides the user with increased functionality when compared to other fluorimeters. Over 200 fluorimeters are now in the field with users reporting excellent datasets.  Applications include in-situ chlorophyll a &  algae class studies, environmental monitoring, dye tracing, particulate studies, cell culture monitoring and process control. It is ideally suited to Algal studies, chlorophyll a monitoring as well as particulate studies.

The innovative TriLux range of digital, in-situ, multi-wavelength, fluorimeters provides the user with increased functionality when compared to other fluorimeters. Chelsea Technologies Group has used expertise gained within its Life Science Group to employ manufacturing techniques that have resulted in a significant reduction in production costs, providing the marine, fresh water, surveying markets with a low cost, high performance sensor.

The TriLux operates in a different fashion to a single wavelength fluorimeter, providing information about the proportion of different light harvesting pigments in the algal sample being interrogated. The TriLux operates on the principle that energy absorbed by the light harvesting pigments is rapidly transferred to Chlorophyll a, where it is used to initiate a cycle of photosynthesis. A proportion of the energy absorbed, however, is re-emitted as Chlorophyll a fluorescence with a peak at 682nm.  All versions of TriLux come with a Chlorophyll a channel as standard, two other channels can then be selected between turbidity and phycoerythrin and phycocyanin - pigments that are prevalent in many cyanobacteria based algae. The TriLux allows the user to assess the relative contribution to Chlorophyll a fluorescence emission from the different light harvesting pigments absorbing light at each of the chosen excitation wavelengths. This information can then be used to make an assessment of the different classes of phytoplankton present in the sample.  It should be noted that different combinations of pigments will be excited at each of the excitation wavelengths offered.

Two signals can be obtained from the standard TriLux: the first is a digital RS232 output in engineering units, the second a 0V to 5V volt calibrated analogue output derived from the digital signal. A single RS422 output is also available as an option. This flexibility makes the TriLux suitable for integrating into many different systems and platforms.

A Windows based Graphical User Interface (GUI) is provided that allows the user to both plot and record time stamped data when operating the TriLux directly from a PC and gives control over many instrument parameters, including: sampling rate, calibration factors and dynamic range.

Although small and low cost, the TriLux fluorimeter maintains the performance standards and build quality previously maintained by the Chelsea Technologies Group’s range of in-situ fluorimeters.

  • Chlorophyll a, Phycoerythrin, Phycocyanin
  • Chlorophyll a, Turbidity, Phycoerythrin
  • Chlorophyll a, Turbidity, Phycocyanin

  • Algae class studies
  • Chlorophyll a monitoring
  • Environmental monitoring
  • Cell culture monitoring
  • Particulate studies
  • Moored, profiled, towed or ROV / AUV platforms

  • Miniature, low cost multi-wavelength fluorimeter
  • Range of excitation wavelength configurations available
  • Chlorophyll-a flourescence detection of 685nm
  • Digital output in engineering units
  • Additional analogue output as standard
  • User selectable sampling rate, 0.1Hz-3Hz
  • Low power consumption
  • Internal referencing of excitation intensity
  • Rejection of ambient daylight
  • High rejection of ambient daylight
  • Low Noise, high sensitivity
  • Low turbidity breakthrough
  • RS232 & analogue output (or RS422 option
  • User adjustable dynamic range
  • Acetal C housing

The Chlorophyll a channel is calibrated for a 0-100 μg/L range (Chlorophyll a in acetone), with the other fluorescence channels being normalised to this calibration. The turbidity channel (if present) is calibrated to a 0-100FTU range.

Performance

  • Dynamic range: Calibrated for 0-100ug/L (chlorophyll a in acetone). User can adjust range by selecting LED output intensity
  • Limit of detection: Typically 0.1% of full range
  • Detection wavelength: 685nm
  • Excitation wavelengths:
    • 470nm - Chlorophyll a
    • 530nm - Phycoerythrin
    • 610nm - Phycocyanin
    • 685nm - Turbidity
  • Available parameters:
    • Chlorophyll a, Phycoerythrin, Phycocyanin
    • Chlorophyll a, Turbidity, Phycoerythrin
    • Chlorophyll a, Turbidity, Phycocyanin

Note: Phycoerythrin and Phycocyanin data from TriLux is related to the Chlorophyll a fluorescence emission. Users who require Phycoerythrin and Phycocyanin concentration data may require the individual CTG UniLux fluorometers.

Mechanical

  • Size: 26.5mm dia x 105mm (140mm to end of connector)
  • Weight in air: 100g
  • Pressure housing: Acetal C
  • Depth rating: 600 metres
  • Connector: MCBH-6-MP-SS

Electrical

  • Input voltage: 11 to 25Vdc
  • Data output: Digital RS232 and analogue 0 to 5Vdc (RS422 and SDI-12 options)
  • Power requirements:

Delivery Package: The standard UniLux/TriLux fluorometer is supplied with transit case, handbook and Windows based Graphical User Interface (GUI).

Accessories: User to specify cables and other accessories.
In view of our policy of continual improvement, the designs and specifications of our products may vary from those described.

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