ClearView is an innovative software package that eliminates interference from complex baselines in GC/MS chromatograms.
Who should use ClearView?
ClearView is ideal for any analyst needing to avoid the time-consuming manual baseline removal and re-integration of GC/MS chromatograms.
How does ClearView work?
How it worksClearView uses dynamic baseline compensation algorithms to distinguish between ions that contribute to chromatographic peaks and those that are just baseline anomalies. This enables the selective removal of interferences such as GC column bleed, humps of unresolved sample matrix, extended solvent tails and air ingress.
What benefits does ClearView offer?
- ClearView brings the following benefits to the GC/MS analyst:
- ClearView improves sensitivity by removing baseline anomalies
- Cleaner baselines make identification of trace target analytes easier
- Integration is simplified, improving quantitative accuracy
- The need for manual re-integration is eliminated, reducing costs and boosting lab throughput
- Files are processed rapidly, either individually or as a batch
- Original GC/MS files remain intact for comparison or future reference
What you can do...
- Remove GC interferences
- Find trace-level compounds hidden under background signals
- Simplify peak integration
- Improve quantitative accuracy
- Eliminate time-intensive manual processing
- Process large numbers of files quickly
- Reduce laboratory running costs
Dynamic background compensation
ClearView software uses sophisticated dynamic background compensation (DBC) algorithms to distinguish between chromatographic peaks and baseline anomalies.
These algorithms examine profiles of individual ions, and identify those that don’t match the profiles of chromatographic peaks. These ions can then be eliminated from the total ion chromatogram (TIC), improving both spectral purity and peak integration. Importantly, this happens without compromising the ions contributing to chromatographic peaks.
By minimising GC/MS background ion contributions, ClearView reduces noise and increases sensitivity in full-scan mode. In the following example, thiophene has a signal-to-noise ratio of approx. 3:1 (RMS) in the original GC/MS chromatogram, versus 30:1 in the ClearView-reprocessed file. This represents a significant increase in sensitivity.
TD–GC/MS analysis of landfill gas, showing extreme column bleed. Original TIC data shown (black) together with ClearView reprocessed data (blue).
Automatic analysis of the original chromatogram does not correctly identify the trace component at 16.486 minutes because high-level background ions interfere with the mass ion fragmentation pattern.
Automatic analysis of the ClearView-reprocessed chromatogram shows greatly enhanced spectral purity, allowing automatic identification of this trace component as thiophene.
By minimising GC/MS background ion contributions, ClearView provides a flatter baseline, facilitating more accurate and repeatable automatic integration, as shown by the following example.
Total ion chromatogram (TIC) showing trace levels (3 pg on-column) of octafluoronapthalene (OFN) in diesel using high-speed chromatography (5 minute run time) and time-of-flight (TOF) MS. The original GC/MS data (black) is shown overlaid with ClearView-reprocessed data (blue).
Diesel data shown in extracted-ion mode. Note identical peak areas for the OFN signal at m/z 272, indicating that peak-related information is not lost using ClearView.
Three overlaid repeat high-speed GC/TOF MS analyses of OFN-spiked diesel, all reprocessed using ClearView. The full run (top) and expansion (bottom) show excellent integration (baseline assignment) and repeatability.
When the original data from this series of three analyses is overlaid (i.e. as above, but without ClearView reprocessing), the reduction in integration accuracy (baseline assignment) and repeatability become clear.