Simultaneous Analysis of Aflatoxins and Ochratoxin A - Sample Preparation
Aflatoxins and Ochratoxin A are produced by fungi, e.g. Aspergillus and Penicillium species. Therefore both toxin types are found together in many foods and animal feeds, e.g. cereals. Thus it is appropriate to analyze one extract for the occurrence of both mycotoxins in a single step.
A significant assistance is the cleanup of extracts by a combined immunoaffinity column for both mycotoxin types in one step. The subsequent analysis may then be performed by HPLC with post-column derivatization or LC-MS.
Pickering Laboratories’ combination sample preparation column Afla-OtaClean from LCTech provides an ideal immunoaffinity column. This combination column is very tolerant towards many matrices and allows for a comprehensive cleanup of the aflatoxins B1, B2, G1 and G2 as well as Ochratoxin A. The high maximum capacity of 150 ng for Aflatoxin B1 and Ochratoxin A provides a wide a measurement range.