Multiplex Displacement - Case Study

Feb. 6, 2023

Problem
Customer facing challenges getting desired multiplex editing result, and wanted to investigate the cause.

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CRISPR-Chip analysis was used to characterize the binding kinetics of different gRNAs to Cas.

CRISPR QC determined how different gRNAs compete for Cas binding sites. In this study, gRNA #5 proved to be the fastest displacer.

Insight provided by CRISPR QC has given the customer new factors to optimize their multiplex editing.

Data