Advansta Inc. products

AdvanStain Iris is a rapid and sensitive alternative to Ponceau S stain for protein detection on Nitrocellulose and PVDF membranes after transfer from polyacrylamide gels. The dark blue color provides a high contrast which facilitates the acquisition of visible images. The dye may also be imaged with epi blue light.

AdvanStain Total is a fluorescent protein stain for PVDF and Nitrocellulose membranes that allows for sensitive and quantitative visualization of protein bands. For precise quantitative Western blot data, it is critical to perform normalization to account for sample inconsistencies, sample loading errors and uneven transfer. In an effort to increase transparency, total protein normalization is quickly becoming the standard for Western blot normalization as it is now the preferred method for top tier journals. The AdvanStain Total Fluorescent Protein Staining Kits provide a convenient method for total protein normalization that is compatible with digital imaging systems equipped with a Cy3-compatible filter.

AdvanBlock-EIA is a novel blocking solution, optimized to enhance specific antibody-antigen interactions for ELISA. This all-in-one blocking solution and antibody incubation buffer is designed to decrease non-specific binding caused by low quality antibodies and serum matrix effects. Provided as a convenient ready-to-use solution intended to directly replace other commonly used blocking buffers.

AdvanStain Scarlet is a fluorescent protein stain for gels and blots that allows sensitive and quantitative visualization of protein bands. With a quick and easy protocol, gels can be stained in less than 3 hours, and imaged on a wide variety of imaging systems. Gels and blots stained with AdvanStain Scarlet can be imaged on any fluorescence imaging system, including laser- and CCD-based systems, with a detection limit of less than 1 ng of protein per band or spot. Fully compatible with downstream Western blotting or mass spectrometry, AdvanStain Scarlet is non-toxic and biodegradable, for safe and simple disposal.

These ready-to-use Laemmli loading buffers contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis. Choose from reducing or non-reducing buffers, each supplied as a 2X concentrate.

In less than 5 minutes, completely remove protein from aqueous solutions of single- and double-stranded nucleic acids. RapidClean is a novel affinity resin designed to remove all protein from aqueous solutions of nucleic acids, providing an alternative to hazardous and lengthy phenol-chloroform procedures. The RapidClean resin binds and removes protein in a 5-minute protocol that combines convenience, speed, and nucleic acid recovery rates in excess of 95%.