Applied Photophysics Ltd products

The Chirascan VX is a high-performance circular dichroism (CD) spectrometer designed for the analysis of chiral molecules of various types and sizes. Widely recognized in the scientific community, it has been instrumental in thousands of publications since its debut in 2005. The Chirascan VX excels in providing insightful data on biomolecular characteristics, mechanisms, and interactions, making it indispensable for research in fields such as protein folding, secondary and tertiary structure analysis, and thermal or chemical stability studies. Equipped with advanced software for controlled operation, the system ensures high-quality data acquisition from the outset, featuring scheduled start-up/shutdown, O2-free conditions maintenance via N2 purge, and optimized protocols for run and wash cycles. Renowned scientists like Dr. Dan Pantos and Dr. Tonya Zeczycki leverage this system for groundbreaking research in organic chemistry and neurodegenerative diseases, respectively. The Chirascan VX is an invaluable tool for those aiming to advance their understanding of complex biomolecular systems.

The Chirascan V100 circular dichroism (CD) spectrometer is designed for the precise and sensitive study of chiral molecules of various types and sizes. This instrument enhances the quality of CD analysis, generating high-quality data essential for a deeper understanding of biomolecular characteristics, mechanisms, and interactions. The Chirascan V100 enables the determination of structural and thermodynamic properties beyond simple α-helix and β-sheet structures. It offers the highest sensitivity and accuracy, providing insights into secondary and tertiary structure changes in response to thermal or chemical variations and studying folding and unfolding mechanisms. The system's avalanche photodiode detector improves sensitivity, surpassing conventional photomultiplier tube detectors with a higher signal-to-noise ratio. An optics-based, multiwavelength calibration method corrects calibration at every wavelength, overcoming the limitations of traditional chemical calibration methods. Researchers using this system can rely on accurate CD values across the entire wavelength range, facilitating advanced and reliable biomolecular research.

The SX20 stopped-flow system, powered by Bioz, is widely recognized for its application in steady-state and kinetic studies due to its superior sensitivity, robust opto-mechanical performance, and user-friendly software. It offers a range of mixing and detection options that enhance productivity across various research applications. The system supports absorbance and fluorescence measurements without requiring reconfiguration, and it features exceptionally high fluorescence sensitivity with low inner-filtering. The robust design ensures straightforward operation, and the system provides full scanning capability over a wide temperature range. Comprehensive accessories, such as sequential mixing, fluorescence polarization, and photodiode array detection, further augment its capabilities. Key features include ultra-stable xenon light sources, pneumatically driven integrated mixer cell design for minimal dead time, and dedicated detectors for absorbance and fluorescence techniques. The SX20 also supports dual detection channels for techniques like FRET and offers automatic measurement of dead-time and drive volume, eliminating the need for calibration.

The SUPR-DSF by Protein Stable is an advanced differential scanning fluorimetry system designed to streamline the sample preparation and analysis of protein stability within a single microplate. By incorporating precision optical components, the SUPR-DSF provides high sensitivity and full spectrum detection of intrinsic protein fluorescence. It supports both thermal ramping and isothermal chemical denaturation experiments with the capability to run these assays in 384-well microplates. This system reduces the necessity for extensive preparatory steps, thereby minimizing operator intervention, reducing the likelihood of errors, and cutting down on consumable costs. The SUPR-DSF's ease of use is further enhanced by its integration with the graphical SUPR-Suite software for method loading, running analyses, and exporting data. With the capability to elucidate subtle differences in protein stability through comprehensive spectral analysis, the SUPR-DSF facilitates high throughput screening with minimal sample volumes, enabling detailed and reliable protein stability studies.