BellBrook Labs products

The Transcreener ADP2 Assay with FP readout measures ADP via antibodies selective to ADP and a far-red fluorescent tracer. ADP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any kinase in a high throughput format. The assay produces an excellent signal at less than 10% ATP consumption for a broad range of initial ATP concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated ADP detection method available backed by the best technical support in the industry.

The Transcreener GDP Assay with FP readout measures GDP via antibodies selective to GDP and a far-red fluorescent tracer. GDP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any kinase in a high throughput format. The Transcreener Assay offers reagent stability and compatibility with commonly used multimode plate readers. The FP GDP Assay accommodates GTP concentrations ranging from 1 µM to 1,000 µM with excellent data quality (Z` ≥ 0.7) at low substrate conversion (typically 10-30%). Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated GDP detection method available backed by the best technical support in the industry.

The Transcreener GDP Assay with FP readout measures GDP via antibodies selective to GDP and a far-red fluorescent tracer. GDP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any kinase in a high throughput format.

Corning® 384-well black plates are a great fit for Transcreener FP and FI assays. They feature low working volume to conserve precious enzyme. BellBrook Labs recommends a final volume of 20 µL for these plates. Black plates absorb light and reduce background as well as well-to-well cross talk. Square well tops enable easier robotic handling, while round bottoms minimize trapped air and provide better assay Z`. Polystyrene non-binding services (NBS) is Corning`s proprietary treatment which limits protein binding at low concentrations. The microplate is non-sterile and comes without a lid. However, for long incubation periods, BellBrook Labs recommends covering your sample wells to reduce evaporation.

Corning® 96-well half area plates are a great fit for Transcreener FP and FI assays. They feature low working volume to conserve precious enzyme. BellBrook Labs recommends a final volume of 50 µL for these plates. Black plates absorb light and reduce background as well as well-to-well cross talk. Polystyrene non-binding services (NBS) is Corning`s proprietary treatment which limits protein binding at low concentrations. The microplate is non-sterile and comes without a lid. However, for long incubation periods, BellBrook Labs recommends covering your sample wells to reduce evaporation.