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Acanthamoeba is one of the most frequent protozoa found in nature. The life cycle of Acanthamoeba species involves two stages: an active form or trophozoite and a latent form known as a cyst. The amoebas of the Acanthamoeba genus have the ability to live as microorganisms of free life in nature and occasionally invade a host and live as parasites within it. The most common types of infections caused by Acanthamoeba spp. they include EAG, disseminated infections (cutaneous and nasopharyngeal) and keratitis.

Candidatus Mycoplasma haematoparvum (CMhp) has been described in association with anaemia in dog and PCR assays have been described for the detection of CMhp genes in blood samples.