Biopremier products

The Biopremier Microbial DNA extraction Kit – Bead beating is designed for a fast and efficient purification of microbial DNA (gram-negative and gram-positive bacteria, yeast and fungi).

The Biopremier DNA Extraction Kit from Food provides an efficient procedure for the isolation and purification of total DNA from food samples (raw material and processed food of animal or plant origin) and bacteria. After the food samples have been homogenized, the DNA can be extracted with the lysis buffer. In case to detect bacterial DNA in food samples, a pre-enrichment with the appropriate culture medium is recommended. The bacterial DNA can be extracted with the lysis buffer, after a concentration step by centrifugation. Lysis occurs by thermal shock and the purification by a series of processes to remove contaminants and residual cellular debris. The clear supernatant is then mixed with the binding buffer, to create conditions for optimal binding to the silica membrane column. The purified DNA is ready-to-use for subsequent reactions like real-time PCR detection and quantification.

The BIOPREMIER Rapid DNA extraction buffer provides a simple way to prepare and extract samples for bacterial DNA testing. A pre-enrichment with the appropriate culture medium is recommended and the bacterial DNA can be extracted with the lysis buffer, after a concentration step by centrifugation from samples in food, water, feed etc. in accordance with the standard methods (ISO 6579, ISO 11290, ISO 16654 and so on). Lysis occurs by thermal shock and a centrifugation step removes part of residual cellular debris. The supernatant could be used directly for subsequent reactions like real-time PCR detection or after a dilution step to remove residual PCR inhibitors.

Detection of E. coli serotypes O157, O26, O111, O103, and O145 in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136 is recommended or from E. coli isolated colonies. For use in real time instruments with FAM, and ROX channels.

Detection of L. monocytogenes in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO 11290 (recommended). For use in real time instruments with FAM and ROX channels.