CD Genomics articles

Introduction to Single-Cell RNA Sequencing

Single-cell RNA sequencing (scRNA-seq) represents a groundbreaking advancement in genomic techniques, allowing researchers to detect and quantify messenger RNA (mRNA) molecules at the single-cell level. This capability is crucial for understanding cellular responses and the intricate dynamics of biological systems. Traditional RNA sequencing methods typically analyze bulk sampl

In the rapidly evolving field of genomics, sequencing technologies play a crucial role in advancing our understanding of genetic material. Among the most prominent methods are Illumina sequencing and nanopore sequencing, each offering unique advantages and capabilities that cater to different research needs.

What is Illumina Sequencing?

Illumina sequencing, a hallmark of second-generation sequencing technology, has revolutionized genomics research. This met

The advent of next-generation sequencing (NGS) technologies and advanced sequencers has led to an unprecedented surge in biological data generated by the scientific community. This explosion of information presents both remarkable opportunities and significant challenges for researchers. In this era of big data, bioinformatics has emerged as a crucial field, making substantial strides in the analysis and interpretation of complex biological datasets. As a result, there is a pressing need for

Microbiome research has significantly advanced our understanding of the complex microbial communities that inhabit various environments, from the human gut to diverse ecosystems. At the forefront of this exploration are two pivotal techniques: 16S rRNA sequencing and metagenome sequencing. Each method offers unique strengths and applications, enabling scientists to unravel the intricacies of microbia

Viruses

Viruses, these minuscule and seemingly simple structures, are garnering increasing attention from scientists worldwide. With their unique characteristics and profound impact on living organisms, viruses are a source of fascination and complexity that extend far beyond their diminutive size.

They are composed of a nucleic acid core, either DNA or RNA, encased within a protein coat. Unlike cellular organisms, viruses lack the essential cellular machinery for

Microorganisms have different growth requirements. When culturing them in the laboratory, it is essential to take note of these conditions because these are needed to be replicated in order to successfully grow microbial cultures in the laboratory. As mentioned in the previous article, one of the conditions needed to be taken into consideration is the oxygen requirement of the microorganisms. Some common microorganisms such as Escherichia coli and Bacillus sp. are usually

What is Chimeric RNA?

Chimeric RNA, also known as fusion RNA, refers to RNA molecules that are formed as a result of the fusion of two or more different RNA transcripts. Chimeric RNA, a unique class of RNA molecules formed through various molecular mechanisms, such as trans-splicing, read-through transcription, and fusion genes. These hybrid transcripts play a significant role in diverse biological processes and have gained substantial attention in recent years.

Fu

What is CRISPR-Cas9?

CRISPR-Cas9 is a gene editing technology that has changed the landscape of biological research. It stands for Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-Associated Protein 9. CRISPR-Cas9 uses a two-component system consisting of a guide RNA (gRNA) and a Cas9 protein. The gRNA is designed to complement specific target sequences in the genome to guide the

Exosomes are small extracellular vesicles that contain RNA, proteins, and lipids. These vesicles play a crucial role in intercellular communication and have been implicated in various physiological and pathological processes. Exosome RNA-Seq is a powerful technique that can be used to study the RNA content of exosomes and gain insight into their function and regulation. In this guide, we will provide an overview

The Relationship Between Exosomes and Cancer

Exosomes are nanoscale vesicles derived from intracellular lysosomes that shuttle a variety of biomolecules, such as nucleic acids, proteins, lipids, amino acids and metabolites, and play a key role in regulating intercellular communication. Exosomes can be secreted by almost all types of cells, including immune cells, blood cells, neuronal cells, epithelial cells, and cancer cells. Exosomes contain various types of RNA molecules, including