Cell Guidance Systems Ltd. products

Exo-spin™ products provide a proven, rapid and reliable way of generating high-quality purified exosomes suitable for a range of research applications. Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.

ExoLISA™ assays are time-resolved immunofluorescence assays which have been developed to measure the abundance of CD9, CD63, or CD81 protein specifically associated with exosomes in biological fluids. The assay provides clear and consistent results in a simple 4-step fluorescent detection process. It is similar to an ELISA assay, but the target is detected directly with a fluorophore label without the need for sample lysis.