Cell Technology products

Adenosine Triphosphate (ATP) is an organic molecule which is exclusively formed in the mitochondria. Virtually all living systems store energy in the phosphate bonds of ATP and plays a central  role in cellular metabolism and energy transfer reactions. ATP and be de-phosphorylation to ADP and further de-phosphorylation to AMP. This cycling of ATP is central to transferring potential (thermodynamic) energy from one source to another. There are several genetic disorders that effect the generation of ATP in the mitochondria (1-3). Cell Technology’s Fluoro ATP assay provides a reliable, sensitive fluorimetric assay for the quantification of ATP in  biological samples.

Adenosine diphosphate (ADP) is an organic molecule which plays a central  role in cellular metabolism and energy transfer reactions. ADP is a product of ATP de-phosphorylation and can be rephosphorylated to ATP or further de-phosphorylation to AMP. This cycling of ADP is central to transferring potential (thermodynamic) energy from one source to another. Besides its involvement in glycolysis, citric acid cycle and oxidative phosphorylation, ADP is also stored as dense bodies in blood platelets. Upon platelet activation, ADP is release which leads to further platelet activation through APD receptors (1-8). Cell Technology’s Fluoro ADP assay provides a reliable, sensitive fluorimetric assay for the quantification of ADP in  biological samples.

Key Benefits: Safe – Non Radioactive Enzyme release assay. Versatile – Useful for measuring activity of T Cells, Primary Cells, NK, complement and other lytic agents. Assay can be run in serum supplemented media. Homogenous – One-step, no wash assay. Assay can be run in same plate as samples. FAST – Results in 3-5 minutes. Chromium 51 or europium release for measurement are time consuming. The inherent sensitivity of luciferase detection is enhanced by the amplification effect of enzyme turnover, which produces thousands, millions or billions of high – energy molecules for each molecule of the enzyme. Highly Sensitive – Can detect fewer than 500 cells/well in the presence of serum or as few as 10 cells/well in serum-free or heat-killed media.

Key Benefits: No radioactive materials required. Applications – Flow cytometry or Fluorescent microscopy. Detects cytolytic activity at a cellular level. Works with multiple types of mammalian cell lines.

Key Benefits: Cell permeable. No wash homogenous assay. Adaptable to High throughput assay platforms. Real time detection of NO activity. Applications - Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy.

Key Benefits: Cell permeability allow direct measurement of apoptosis and mitochondrial potential in live cells. Applications - Cells can be analyzed by Flow Cytometry, Fluorescent plate reader or Fluorescent microscopy. Incubate for 15 minutes, wash and measure. Add this reagent directly to live cells in your media of choice.

Bottle Size: 100 mL, 500 mL, 1 L, 10 L. Increases the Sensitivity of the Assay. This is done by stabilizing the antigenic and functional regions of the absorbed protein. Reduces Background Noise. Block buffers inhibit nonspecific binding to uncoated regions of the plate and non-specific binding sections of the absorbed proteins. This leads to lower background noise and high specific signal. Increases Efficiency and Extends the Shelf-Life of the Coated Plates. With a longer shelf-life, plates may be prepared in large batches to be used over time. Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency. Conserves Valuable Reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.

Bottle Size: 100mL, 500mL, 1L, 10L. Saves valuable reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used. Increases the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at room temperature or 2°-8°C for several months, or even years under proper conditions. Increases reproducibility. By using consistent reagents from a reliable source, your ELISAs may become more reproducible. Increases the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.

Bottle Size: 100 mL, 500 mL, 1 L, 10 L. Increases the Sensitivity of the Assay. Decreases Backgrounds. Stabilizes Newly Conjugated Proteins. Also reconstitutes lyophilized conjugates and diluents concentrates conjugates. Prolongs the Shelf-Life of HRP-conjugated proteins and enhancing their utility in ELISA applications. Extends the Useful Titer of the conjugate. Conserves Valuable Reagents. By promoting a higher specific signal, less conjugate may be needed to generate a signal when a stabilizing conjugate diluent is used. Lengthens the Shelf-Life of conjugated antibodies for future use. With a longer shelf-life, conjugates may be prepared in batches for use in future plate coating runs. Using conjugates from the lot generally reduces inter-assay variability and increases consistency.

Bottle Size: 100 mL, 500 mL, 1 L, 10 L. By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.