Claret Bioscience products

SRSLY - our Single Reaction Single-stranded DNA Library Prep Kit. SRSLY is a simple and efficient ligation-based single-stranded DNA library preparation method that is engineered to produce complex libraries from samples with limited inputs. SRSLY recovers complete double-stranded DNA molecules, even when they contain nicks, plus single-stranded DNA molecules inaccessible to most NGS prep kits. Furthermore, the freedom of choice during DNA cleanup allows the user flexibility to decide whether they want to retain or discourage a substantial portion of short fragments. Did we mention it does all of this, from sample to sequencer, in under 3 hours without altering the native ends of cell-free DNA molecules? Seriously.

XACTLY provides comprehensive information about the native-state of fragmentary DNA termini including the type (5´, 3´ , or blunt), length, and sequence of all single-stranded overhangs. The generation of blunt ends or overhanging termini can result from physical, chemical, or enzymatic cleavage, physiologic processes or pathological mechanisms. The native termini of DNA molecules reveal information about where they have been and what they have interacted with. We initially developed XACTLY to interrogate cell-free DNA degradation. While our internal cfDNA research is ongoing, we’ve also found many other exciting applications for XACTLY including: identifying restriction enzyme cut sites, nuclease digestion preferences, and better understanding DNA degradation over time.