Cube Biotech GmbH products

The Cube Biotech GentleLys Buffer is the only 100% detergent-free, non-denaturing solution to lyse cultured cells from plated cells as well as cells pelleted from suspension cultures while solubilizing all membranes in the process. GentleLys enables the extraction of soluble proteins and the stabilization of membrane proteins from all compartments of the cells. It is compatible with many applications, including Immunoassays, Protein Assays, Mass Spectrometry, and Protein Purification. The Buffer comes in two variants: GentleLys – Dissolve which lyses cells in 15 min while GentleLys – Stabilize lyses all cells and makes the whole proteome (soluble and membrane proteins) of the cell available for your analytic approaches. It solubilizes all membranes and stabilizes expressed membrane proteins in their native conformation They are able to do this thanks to the inclusion of synthetic nanodisc copolymers. More on that can be found here.

Our E.coli cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system. The advantages of cell-free protein expression are numerous, among other things it enables a scientist to:

Every protein / lipid / biomolecule purification protocol that involves magnetic beads does eventually lead to the separation step in the end. As essential as a good MagBead separator may be most lack certain quality features that our PureCube MagBead separator fullfills and even improves upon with additional features: