emp Biotech GmbH products

Gel filtration column for purification and desalting of proteins largern than 5 kDa or nucleic acids longer than 10 bp/nt. Processes sample volumes of 2.5 mL.

For maintaining low water content, suitable for volumes up to 100 mL. Fits bottlenecks with diameter > 12 mm.

Activated Zeolite sachets for rapid reduction of water content in solvents; Contains 0.5 g. For drying volumes of up to 100 mL. Fits bottlenecks with diameter > 12 mm.

TurboTag 682 Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of DY-682 from DYOMICS.  The conjugates result from the formation of a stable covalent amide linkage. The protein-dye conjugates have fluorescence-excitation and fluorescence-emission maxima at around 681 nm and 703 nm, respectively.  Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive TurboTag 682 dye. To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).

The TurboTag 415 Protein Labeling & Purification Kit is designed for the labeling and purification of proteins to form protein-dye conjugates having fluorescence-excitation and fluorescence-emission maxima at around 651 nm and 666 nm, respectively.

Puress PCR-96UF is a fast and cost-effective ultrafiltration kit for purification of PCR amplification products larger than 150 bp. Designed for high throughput applications. Suitable for all vacuum or centrifugation protocols, either manually or in automated workstations.

Gel filtration column for purification and desalting of proteins larger than 5 kD. Processes sample volumes of 150 to 300 µL.

Gel filtration column for purification and desalting of proteins larger than 5 kD. Processes sample volumes of 0.5 mL.

Total hands-on time: 10 minutes, including purification. FluoroSpin Protein Labeling and Purification Kits are designed for precision labeling of antibodies, enzymes or proteins having a molecular weight greater than 25 kDa. Labeling is accomplished using the reactive N-hydroxy-succinimidyl ester (NHS) of a fluorescent dye.

For each synthesis cycle, up to 1 to 2 % of free 5’-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent „Capping“ step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.