KDBIO S.A.S. products

A FiberCell Systems Hollow Fibre Bioreactor gives any laboratory the possibility to significantly scale-up cell culture and generate very large amounts of cell-secreted products. The aim with this kind of system is to establish a steady-state high density culture that generates a very consistent product. Data is available to demonstrate this capability.

HFIM starter package available through KDBIO in Europe: Fibercell DUET Pump and hollow fibre cartridge modules (sterile consumables) which together provide very fast media recirculation for equilibration. FiberCell Reservoir caps : 2 x autoclavable 5-way caps for central reservoir and 4 x autoclavable 2-way caps for diluent and waste

Measuring glucose in cell culture media has never been this simple: Calibrated specifically for mammalian or insect cell culture media (not blood!). Direct measurement (no need to separate cells from medium). 15 second response time. Precision <5%, Accuracy >90%. Sample vol 1.5 µl

20 kD MWCO polysulfone fibre with 450 cm2 of surface area. Only one wrap of oxygenation tubing to reduce system dead volume. Popular application – culture of primary MSCs for production of extracellular vesicles and evaluation studies prior to scale-up (download protocol for MSC culture in hollow fibre bioreactor).

0.1 µm pore size for highest exchange rates but without protein retention. 80 cm2 outer surface area. PVDF fiber allow for the attachment of matrix proteins, cytokines and antibodies. Mentioned in several publications for endothelial cell culture under shear and various types of primary cell culture. Requires wetting with alcohol.