Montana Molecular products

The Green and Red Fluorescent Cell Stress Assay provides a robust method for detecting stress responses in living cells. This assay involves the use of a bright green fluorescent protein that is activated under endoplasmic reticulum (ER) stress conditions or during the unfolded protein response (UPR). Utilizing fluorescence plate readers or imaging systems, these assays enable precise measurement of cellular responses to chemical or genetic stress. The technology enables high-throughput screening for compounds that modulate ER stress pathways, providing insights into mechanisms of neurodegeneration and protein misfolding diseases. Additionally, a ratiometric biosensor variant offers enhanced detection capabilities by measuring both green and red fluorescent signals. This dual color assay is versatile for applications in diverse cell types, including primary cells and induced pluripotent stem cell-derived cells. Packaged in a BacMam vector, it ensures efficient expression and compatibility with imaging systems, contributing to advanced research in cellular stress physiology.

Detect Gs or Gi signaling with cADDis, our cAMP Assay. Use cADDis to measure cAMP through Gs or Gi signaling in real time with a single sensor. Plate reader or imaging system compatible.

Montana Molecular offers a suite of targeted cADDis cAMP assays designed to measure cyclic AMP levels within specific sub-cellular domains of living cells. These assays make use of biosensors genetically engineered to localize within distinct cellular compartments like cilia, nucleus, and plasma membrane, providing high precision and specificity. Variants of the assays are available in both green and red fluorescent versions, allowing for robust visualization and quantification of intracellular cAMP dynamics in response to GPCR activation. The use of BacMam vectors ensures consistent delivery and expression of these genetically encoded sensors across a variety of cell lines, facilitating research in cellular signaling. Specific targeting to AKAPs, membrane sites, and cilia enables researchers to discern the spatiotemporal aspects of cAMP signaling pathways, potentially leading to deeper insights into cellular processes affected by such molecular mechanisms.