Nucleics Pty Ltd. services

The PeakTrace Basecaller is a novel DNA sequencing software for molecular biologists and genetists to improve the quality and read length of automated DNA traces collected on the ABI 310, 377, 3500, 3730, 3100 and 3130 DNA sequencing instruments.

The free PeakTrace Online has been retired and replaced with PeakTrace Online Pro. To register to use PeakTrace Online Pro for free please enter your name, email address, company/institution, and approximate DNA sequencing volume. We will email you the password to access the PeakTrace Online Pro page. This will enable you to test for free the full version of PeakTrace on up to 250 traces per day (worth over $375).

Troubleshooting DNA Sequencing Problems: Automated DNA sequencing is one of the most common and normally most robust techniques performed in molecular biological laboratories. Unfortunately, it does not always work and when it doesn’t it can be very difficult to work out what went wrong. Fortunately, most failed (or sub-optimal) DNA sequencing results have only a limited number of causes. To help the troubleshooting of DNA sequencing problems we have created a series of guides for identifying the most common causes. These guides also includes tips on how to overcome each problem type, along with more general tips for improving DNA sequencing quality.

PeakTrace is a basecaller and improves your DNA sequences, but will not allow you to view the peaks in the .ab1 trace file. To visualize the trace you need a trace viewing program. There are many good trace viewers and most are free. Many come with additional features like manual editing, BLAST analysis, or the ability to view the raw data. To help you find the best trace viewer for your needs we have listed all the trace viewers we are aware that exist. We will be adding reviews of the various programs as we find time.

Reviews of DNA Sequencing Service Companies & Facilities. To help you choose a DNA sequencing service we have reviewed many of the popular DNA sequencing service providers. The service facilities are grouped by geographical location in alphabetical order.

Use clean DNA: The cleanliness of the DNA is the most important factor in the success of automated DNA sequencing. The DNA should be free of proteins, RNA, polysaccharides and genomic DNA. This can best be achieved by using either a commercial plasmid miniprep kit, or by sequencing a PCR amplified fragment.