NZYTech - Genes & Enzymes products

NZYMaxiprep kit is designed for the rapid, large-scale preparation of highly pure plasmid DNA (typically 500 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYTech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (less than 40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 500 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.

NZYMaxiprep, no columns contains all the buffers for maxi plasmid isolation that can be found in NZYMaxiprep (MB051).

NZYTech Plasmid Maxi Columns are components of NZYMaxiprep kit (MB051), sold separately for your convenience.

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents (1% Triton or 0.5% SDS) and it is used for protolytic inactivation of nucleases during isolation of DNA and RNA. Presented as a white lyophilized powder.

NZY Mag Viral RNA/DNA Isolation kit, IVD, is a magnetic bead technology-based nucleic acid purification kit designed to recover RNA and DNA from viral particles contained in transport medium from human nasopharyngeal swabs. The kit uses magnetic-bead technology to provide reproducible recovery of high-quality nucleic acids. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions.