Sartorius BIA Separations products

The CIMmultus HiP² Plasmid Process Pack is designed for the purification of plasmid DNA (pDNA), aiding scientists in developing bespoke purification processes that can transition seamlessly to production environments. This adaptable kit includes a weak anion-exchange column (DEAE) and a hydrophobic column (C4 HLD), providing flexibility within its protocols to suit specific plasmid purification needs. The pack is equipped with comprehensive experimental instructions, streamlining the setup process and reducing the time and financial investment required at various scales. Standard connector compatibility with most liquid chromatography systems is ensured, although special adapters are available if needed. This process pack is an essential tool for those looking to refine and implement plasmid purification methods efficiently.

The In-line Alkaline Lysis System offered by Sartorius is a state-of-the-art, automated solution designed for efficient process development and pilot-scale operations. It features a closed single-use loop system capable of processing up to 500 liters of resuspended bacterial cells per workday. Integrating advanced mixing controls allows for continuous operation, enhancing the isolation of plasmid DNA by minimizing contamination from host cell genomic DNA and RNA. The system comprises the Lysis Reactor and the Alkalizator Tower, which collectively streamline the lysis process. The Lysis Reactor, a single-use component, is designed for ease of installation and operation in compact spaces, providing an effective alternative to traditional batch lysis methods. The Tower features user-friendly software, four peristaltic pumps, and ultrasonic flow sensors for precise control, alongside essential safety precautions like non-contact pressure sensors to ensure reliable performance.