Stilla Technologies software

Crystal Miner software automatically identifies positive and negative droplets in all the fluorescence channels of interest and measures the concentrations of targeted nucleic acids. With its intuitive visuals, Crystal Miner software eases the analysis of complex and highly multiplexed assays.

The heatmap visualization is a convenient analysis tool to delimit 2D zones. In heatmap visualization mode, the colormap represents the different droplet density levels in the fluorescence populations. Droplet densities are visualized utilizing a color gradient displayed on the second y axis. Low droplet densities are represented by shades of colours on the lower axis (light grey) while increasing droplet densities are color coded in shades of blue, green, yellow and red representing the highest droplet density.

Crystal Digital PCR™, the next generation of DNA amplification, is based on a patented technology to partition the fully assembled PCR sample mix into thousands of individual reaction compartments prior to amplification, in a single step. Nucleic acid molecules are thus distributed across partitions, and amplified separately, alleviating any potential competition, and enabling absolute quantification of the DNA or RNA molecules.