4LAB Automatic Pipetting System

Automated PCR/qPCR Sample PreparationLABPCR/qPCR Sample Preparation4titude's® 4LAB™ is an automated, high-precision pipetting system specifically designed for low-volume PCR/qPCR sample preparation. For good PCR results, small volumes of reagents need to be dispensed accurately and consistently.The 4LAB™ was designed as a replacement for manual PCR/qPCR sample preparation, which is tedious, time consuming and prone to human error. Accuracy, precision and consistency are guaranteed and reagent wastage is reduced. Unlike complex, multi-purpose robotic systems, our instrument was designed for the researcher without prior robotic experience. Intuitive set-up and programming will save your laboratory time and money straight away.Easy and Convenient to Use• PC software can be mastered in an hour. No trained technician required.• Built-in PCR/qPCR set-up protocols can be quickly modified and transferred via USB memory stick.• Single and 8-channel, 50µl or 200µl automated pipetting modules can be changed without tools. • 4 interchangeable standard microplate / tip rack adapters and 2 interchangeable reagent vessel adapters available.• Other applications include sequencing set-up, HLA typing and SNP detection, etc.Affordable Solution• The most affordable automated pipetting system available.• Robotic tips are compatible with the Beckman® Biomek® NX, FX & 3000.• Cooling block adapters keep sensitive reagents / samples at 7ºC for over an hour.• Save reagent costs by human error reduction and using low volume PCR plates.Easy to Service• The automated pipetting modules are simple to remove and can be returned for service and calibration.• PC software is upgraded via the internet.• The entire system is compact and light.Accurate and Precise• Each Automated Pipetting Module (APM) is calibrated using ISO-8655 standards.• Excellent results for qPCR standard curve and replicates (Figure 1).• Better Precision of pipetting results than manual pipetting (Figure 2).Figure 1. Excellent Serial Dilution Results. 7µl NIH/3T3 Cell cDNA sample is diluted with 21µl water at 1:4 ratio, 4 times. Roche® LightCycler® 480 realtime PCR thermal cycler and Finnzyme DyNAmo® Flash SYBR® Green qPCR kit (F-415L) were used.Figure 2. Superior Precision Results vs Manual Pipetting Target: Human GAPDH (top curve) was amplified in 4 replicates. 18µl master-mix and 2µl cDNA were pipetting into 20µl reaction volume. Roche® LightCycler® 480 real-time PCR thermal cycler and Invitrogen™ Platinum® Taq DNA Ploymerase were used.4LAB™: Ct std dev = 0.135Manual Pipetting: Ct std dev = 0.145Automated Pipetting Module (APM)• Easy to change• Single/8 - channel, 50µl or 200µl models• Automatic channel and volume identificationFunctional Safety Housing• Safety door detection for emergency stop• Light acrylic materialDisposable Used Tip Tray• Capacity > 300tipsReagent Area• 2x4 2ml or 1.5ml microtubes• 6x2ml free standing tubes & 1x5ml bottle• Cooling block available for various reagent vessels4-position SBS Worktable• 1 or 2 tip-racks• 2 or 3 adaptors for PCR plates/stripes• Cooling blocks available for 96 or 384-well PCR platesCompact Design• Small footprint• Extremely lightNotebook Computer with Software• PCR/qPCR-oriented software• Pre-run simulation• Windows® 7 operating system • 15.6" monitor4ti-2000 4LAB™ Automated PCR/qPCR Sample Preparation System with Notebook Computer and Control Software. 14ti-2010 50µl Single Channel Pipetting Module 14ti-2020 50µl 8-Channel Pipetting Module 14ti-2030 200µl Single Channel Pipetting Module 14ti-2040 200µl 8-Channel Pipetting Module 14ti-2051 96 Tip Rack Adapter 14ti-2052 Elevated 96-Well PCR Plate Adapter 14ti-2053 Elevated 384-Well PCR Plate Adapter 14ti-2054 1.5ml Tube Adapter, 4 x 2 14ti-2055 2ml (3x2) & 5ml (1) Tube Adapter 14ti-2056 Cooling Block for 96-Well PCR Plates 14ti-2057 Cooling Block for 384-Well PCR Plates 14ti-2058 Cooling Block for 1.5ml Tubes (4x2) 14ti-2059 Cooling Block for 2ml (3x2) and 5ml (1) Tubes 14ti-2060 20x1.5ml Tube Adapter 14ti-2061 Used Tip Tray, Disposable 104ti-2062 8-Channel Pipetting Reservoir 1Description QtyAccuracy (Rel.) ± 3% ± 0.8%Precision (Rel. CV) = 1% = 0.15%10µl200µl tip on single or 8-channel to pipetting module200µlAccuracy (Rel.) ± 7% ± 1%Precision (Rel. CV) = 7.5% = 0.4%1µl50µl tip on single or 8-channel to pipetting module200µl4ti Product CodeOrdering InformationTechnical SpecificationPerformance of Automated Pipetting Module (APM)Trademarks: Beckman®, Biomek® 3000 (Beckman Coulter Inc.); Roche®, LightCycler® 480 (Roche Group); DyNAmo™ (Finnzyme Oy); SYBR® (Molecular Probes Inc.); Invitrogen™ Platinum® (Invitrogen Corp.)Note: Specifications are subjected to change without notice.Note: According to ISO-8655 standards (Gravimetic method), APM is calibrated in temperature (21~25ºC, ±0.5ºC) and humidity (60~90%) controlled environment. Twice~distilled water, robotic tips and microbalance were used.Worktable Capacity: 2 to 3 x 96 / 384 SBS PCR plates 1 to 2 x 96 tip rack (50/200µl) Reagent Area 1: 8 x 1.5/2 ml microcentrifuge tube Reagent Area 2: 6 x 2 ml storage tube (free standing) and 1 x 5 ml bottleFunction : Liquid (Sample/Reagent) Transfer (LT) Multiple Dispense (MD) Serial Dilution (SD) Hold (Pause) Mixing (MIX) LoopAutomated Pipetting Interchangeable single/8-channel, Module: Maximum volume 50µl/200µlConnection: RS-232, USB2.0Power Supply: 100~240V, 50/60 Hz, 100WSize (W x D x H): 590 x 440 x 460 mmWeight (N.W.): 25 KgUsed Tip Tray 4ti-20611.5ml (20) tube adapter 4ti-206096 tip rack adapter 4ti-20512ml (3x2) & 5ml (1) tube adapter 4ti-20558-Channel Pipetting Reservoir 4ti-2062Elevated 384-well PCR Adapter 4ti-2053Elevated 96-well PCR Adapter 4ti-20521.5ml (4x2) tube adapter 4ti-20544titude® OfficesUK (Head Office)4titude® LtdThe North Barn, Surrey Hills Business Park,Damphurst Lane, Wotton,Dorking, Surrey, RH5 6QT, UKT: +44 (0) 1306 884885F: +44 (0) 1306 884886E: info@4ti.co.ukwww.4ti.co.ukCertificate number 7281QMS001