ACE UltraCore Technical- Brochure

All the proven advantages of ACE UHPLC / HPLC columns... ...now available with solid-core particlesACE UltraCore™EfficiencySelectivityLifetimeInertness• Ultra-inert solid-core UHPLC / HPLC columns• High efficiency 2.5µm and 5µm solid-core particles• SuperC18 and SuperPhenylHexyl phases with extended pH stabilitySolid-Core UHPLC / HPLC Columns2www.ace-hplc.com ACE UltraCore Ultra-Inert Solid-Core UHPLC / HPLC ColumnsACE® UltraCore™ Ultra-Inert Solid-Core Columns with Extended pH StabilityUltra-Inert Solid-Core Particles ¦ 2.5µm and 5µm ultra pure solid-core (superficially porous)1 particles¦ Monodisperse particle distribution combines high efficiency with low pressure¦ Achieve UHPLC efficiency and performance on HPLC instrumentationSuperC18™ and SuperPhenylHexyl™ Phases¦ Two bonded phases give complementary selectivity for rapid, systematic method development¦ Excellent reproducibility and column lifetime¦ Ultra-inert phases ensure excellent peak shapeExtended pH Stability ¦ Exploit selectivity changes at low, intermediate and high pH¦ Designed for use with LC/MS compatible buffers¦ Recommended pH range 1.5 – 11.0Exploit Selectivity by Adjusting pH Application # 1801pH 1pH 2pH 3pH 4pH 5pH 6pH 7pH 8pH 9pH 10pH 11Column: ACE UltraCore 2.5µm SuperC18, 50 x 2.1mmSample: 1) atenolol 2) methylphenylsulphoxide 3) eserine 4) prilocaine 5) bupivacaine 6) tetracaine 7) 1,2,3,4-tetrahydro-1-naphthol 8) carvedilol 9) nitrobenzene 10) methdilazine 11) amitriptyline 12) valerophenoneTemperature: 40°C Flow Rate: 0.60ml/min Wavelength: 254nm Gradient: 3 – 100% B in 5 minutesAcidic Mobile Phase: A: 20mM ammonium formate in H2O (pH 3.0) B: 20mM ammonium formate (pH 3.0) in 90:10 (v/v) MeCN/H2OBasic Mobile Phase: A: 0.1% NH3 (= 18mM) in H2O (pH 10.7) B: 0.1% NH3 (=18mM), pH 10.7 in 90:10 (v/v) MeCN/H2O0 21 3 4 5213pH 3.0pH 10.70 21 3 4 5123Time - Minutes45678, 91011127948612510111Also referred to as Fused-Core®, core-shell, SPP or core enhanced particles.3www.ace-hplc.comACE UltraCore Ultra-Inert Solid-Core UHPLC / HPLC ColumnsACE UltraCore SuperC18 and SuperPhenylHexyl phases are manufactured using our unique Encapsulated Bonding Technology (EBT™). This technology dramatically increases ligand coverage of the silica surface and effectively eliminates the negative effects of unbonded silanol groups. The higher ligand coverage results in improved inertness, chromatographic performance and stability. Comparison of Column Inertness¦ Solid-core columns from leading manufacturers investigated¦ Comparison of column efficiency for pyridine, a basic molecule¦ Efficiency measured at 50% peak heightPeak Efficiency Comparison Reproduced with kind permission of The Open University, UK.ACE UltraCore 2.5 SuperPhenylHexylACE UltraCore 2.5 SuperC18Phenomenex Kinetex 1.3 C18Phenomenex Kinetex 1.7 C18Waters Cortecs 1.6 C18Phenomenex Kinetex 2.6 C18Thermo Accucore 2.6 C18116 bar117 bar450 bar235 bar285 bar157 bar117 barPressure EfficiencyColumn Dimensions: 50 x 2.1mm Sample: 1) uracil 2) pyridine 3) phenol Mobile Phase: 30:70 (v/v) MeOH/10mM NH4OAc in H2O (pH 5.8) Flow Rate: 0.20ml/min Temperature: 22°C Wavelength: 254nmComparative data may not be representative of all applications. Please see back page for acknowledgement of trademarks. ACE UltraCore 2.5 SuperC18N0.5(pyr) = 49,100pl/mWaters Cortecs 1.6 C18N0.5(pyr) = 35,200pl/mPhenomenex Kinetex 1.3 C18N0.5(pyr) = 44,400pl/mP = 117 bar P = 285 bar P = 450 barEndcapping groupUnbonded silanol groupTime - Minutes Time - Minutes Time - Minutes0 1 2 3 0 1 2 3 0 1 2 3Si-OHACE® UltraCore™ Stationary Phases Virtually Eliminate the Negative Effects of Silanols on UHPLC & HPLC SeparationsTraditional C18 Bonding ACE® UltraCore™ SuperC18 with Encapsulated Bonding Technology (EBT™)ACE® UltraCore™ SuperPhenylHexyl with Encapsulated Bonding Technology (EBT™) Encapsulated Bonding Technology (EBT™) for Improved Chromatography and StabilityC18 C18PhenylHexylApplication # 1802120 20,000 40,000 60,000 80,000 100,000N0.5 (pyridine) (plates/m)3123123 71,500pl/m 49,100pl/m 44,400pl/m 35,600pl/m 35,200pl/m 34,800pl/m 19,200pl/mProduct Availability and SpecificationsPhase Functional GroupParticle Size (µm)Pore Size (Å)Surface Area (m2/g)Carbon Load (%)Maximum pH RangeUSP ListingACE UltraCore 2.5 SuperC18 Octadecyl encapsulated 2.5 95 130 7.0 1.5-11.0a L1 ACE UltraCore 2.5 SuperPhenylHexyl Phenyl-Hexyl encapsulated 2.5 95 130 4.6 1.5-11.0a L11ACE UltraCore 5 SuperC18 Octadecyl encapsulated 5 95 100 5.4 1.5-11.0a L1 ACE UltraCore 5 SuperPhenylHexyl Phenyl-Hexyl encapsulated 5 95 100 3.6 1.5-11.0a L11a ACE UltraCore columns are designed for use with LC/MS compatible buffers. Further information is contained within “ACE UltraCore – A Guide to Buffer Selection” - please contact your distributor to request your FREE copy or visit www.ace-hplc.com.To further extend UHPLC and HPLC column lifetimes, ACE pre-column filters are recommended - for further details please contact your distributor or visit www.ace-hplc.com. For HPLC column connections up to 5000 psi, PEEK™ fingertight fittings (p/n ACE-CC10) are recommended - for further details please contact your distributor or visit www.ace-hplc.com. For UHPLC column connections up to 25000 psi, reuseable fittings (p/n EXL-CC10) are recommended - for further details please contact your distributor or visit www.ace-hplc.com.ACE UltraCore 2.5µm SuperC18 UHPLC/HPLC Columns (UHPLC/HPLC hardware format with 1000 bar/15000 psi pressure limit)ColumnDiameterColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mm2.1mm CORE-25A-0202U CORE-25A-0302U CORE-25A-3502U CORE-25A-0502U CORE-25A-7502U CORE-25A-1002U CORE-25A-1202U CORE-25A-1502U3.0mm CORE-25A-0203U CORE-25A-0303U CORE-25A-3503U CORE-25A-0503U CORE-25A-7503U CORE-25A-1003U CORE-25A-1203U CORE-25A-1503U4.6mm CORE-25A-0246U CORE-25A-0346U CORE-25A-3546U CORE-25A-0546U CORE-25A-7546U CORE-25A-1046U CORE-25A-1246U CORE-25A-1546UACE UltraCore 2.5µm SuperPhenylHexyl UHPLC/HPLC Columns (UHPLC/HPLC hardware format with 1000 bar/15000 psi pressure limit)ColumnDiameterColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mm2.1mm CORE-25B-0202U CORE-25B-0302U CORE-25B-3502U CORE-25B-0502U CORE-25B-7502U CORE-25B-1002U CORE-25B-1202U CORE-25B-1502U3.0mm CORE-25B-0203U CORE-25B-0303U CORE-25B-3503U CORE-25B-0503U CORE-25B-7503U CORE-25B-1003U CORE-25B-1203U CORE-25B-1503U4.6mm CORE-25B-0246U CORE-25B-0346U CORE-25B-3546U CORE-25B-0546U CORE-25B-7546U CORE-25B-1046U CORE-25B-1246U CORE-25B-1546UACE UltraCore 5µm SuperC18 UHPLC/HPLC Columns (UHPLC/HPLC hardware format with 1000 bar/15000 psi pressure limit)ColumnDiameterColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mm 250mm2.1mm CORE-5A-0202U CORE-5A-0302U CORE-5A-3502U CORE-5A-0502U CORE-5A-7502U CORE-5A-1002U CORE-5A-1202U CORE-5A-1502U CORE-5A-2502U3.0mm CORE-5A-0203U CORE-5A-0303U CORE-5A-3503U CORE-5A-0503U CORE-5A-7503U CORE-5A-1003U CORE-5A-1203U CORE-5A-1503U CORE-5A-2503U4.6mm CORE-5A-0246U CORE-5A-0346U CORE-5A-3546U CORE-5A-0546U CORE-5A-7546U CORE-5A-1046U CORE-5A-1246U CORE-5A-1546U CORE-5A-2546UACE UltraCore 5µm SuperPhenylHexyl UHPLC/HPLC Columns (UHPLC/HPLC hardware format with 1000 bar/15000 psi pressure limit)ColumnDiameterColumn Length20mm 30mm 35mm 50mm 75mm 100mm 125mm 150mm 250mm2.1mm CORE-5B-0202U CORE-5B-0302U CORE-5B-3502U CORE-5B-0502U CORE-5B-7502U CORE-5B-1002U CORE-5B-1202U CORE-5B-1502U CORE-5B-2502U3.0mm CORE-5B-0203U CORE-5B-0303U CORE-5B-3503U CORE-5B-0503U CORE-5B-7503U CORE-5B-1003U CORE-5B-1203U CORE-5B-1503U CORE-5B-2503U4.6mm CORE-5B-0246U CORE-5B-0346U CORE-5B-3546U CORE-5B-0546U CORE-5B-7546U CORE-5B-1046U CORE-5B-1246U CORE-5B-1546U CORE-5B-2546UACE® UltraCore™ columns are available through our international distributor networkAvailable from:Advanced Chromatography Technologies Limited, 1 Berry Street, Aberdeen, AB25 1HF, ScotlandTel: +44 (0) 1224 704 554 • Fax: +44 (0) 1224 841 301 • email: info@ace-hplc.com • www.ace-hplc.com14000Q/1113/003-A1518V2ACE® is a registered trademark of Advanced Chromatography Technologies Limited. ACE® UltraCore™, SuperC18™, SuperPhenylHexyl™ and EBT™ are trademarks of Advanced Chromatography Technologies Limited. Advanced Chromatography Technologies Limited acknowledges the registered and unregistered trademarks of Advanced Materials Technology Inc., Phenomenex Inc., Thermo Fisher Scientific, Victrex Plc and Waters Corporation and has no affiliation with any of these companies. Solid-Core UHPLC / HPLC Columnswww.ace-hplc.com