Biosense Laboratories AS - V01018401 - Fathead Minnow Vitellogenin ELISA Kit Brochure

Detection of the egg yolk precursor vitellogenin (Vtg) in blood and tissue samples of juvenile and male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with oestrogenic effects (Arukwe & Goksøyr, 2003; Sumpter & Jobling, 1995). Measurement of Vtg has become an accepted routine screening test for oestrogenic and anti-androgenic effects of EDCs in fish. This Enzyme-Linked Immunosorbent Assay (ELISA) can readily be combined with standard fish toxicology tests developed under the framework of governmental organizations, e.g. the OECD and the US EPA. This ELISA is developed for a standard test species used in many ecotoxicology laboratories throughout the world, the fathead minnow (Pimephales promelas).