Bruker - Model microflex series - Brochure

Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice. © BDAL 11-2012. #256045Innovation with IntegrityMALDI-TOF MSPerformance for Screening Applications on the BenchmicroflexEntry-Level to High-Performance Time-of-Flight Mass Spectrometry The microflex is designed to be a com-pact and affordable benchtop MALDI-TOF system that is convenient for up-to-date life science laboratories.PerformanceTaking advantage from Bruker Daltonics‘ long expertise in the design of cutting-edge mass spectrometers, the unique design of the microScoutTM ion source and the gridless reflectron give the microflex superior resolution, excellent mass accuracy and outstanding sensitivity – unequaled in this class.Whether it is biomarker discovery, oligonucleotide analysis or even the innovative fast and reliable identification of microorganisms: The microflexTM is the perfect instrument to answer questions in clinical proteomics, functional genomics and microbiology.Species-specific IDs in minutesDisclose bacteria’s identity by its own unique protein composition with the MALDI Biotyper. During microflex MAL-DI-TOF analysis, proteins and peptides are arranged in a spectrum with increasing mass – revealing a characteristic peak pattern, even for reliable differentiation of species: The individual fingerprint.Ease of operationSingle bacterial colonies or otherbiological material can be applied to a MALDI sample target after a simple sample preparation procedure and subse-quently introduced to the microflex.Spectra acquisition is completed aftera few minutes and data evaluation isdirectly linked to the measurement with MALDI Biotyper in a real-time analysis.This simple and exclusive workflowis adequate for most microorganisms,avoiding the need to perform gramstai-nings, oxidase-tests or to select primers for PCR. A constantly growing, ready-to-use reference library guarantees reliable ID of bacteria, yeasts and fungi. QC of Oligonucleotides and SNP genotypingAs part of the GENOLINKTM package, the microflex is the ideal tool for routine analy-sis of oligonucleotides for genotyping pro-jects and quality control of oligonucleotide synthesis. The GENOLINK platform is dedi-cated to semi-automated SNP genotyping, providing highly reliable results via direct, label-free MALDI-TOF mass spectrometry and data interpretation.Microorganism IdentificationFast and reliable identification of various bacterial species with MALDI-TOF mass spectra profiling using MALDI Biotyper, identified by their individual peptide molecular profile.Clostridia dendrogram created with MALDI Biotyper; finest differences bet-ween species can readily be evaluated. Data were generated in cooperation with Prof. Krüger and Dr. Grosse-Herrenthey, Institute of Bakteriology and Mycology, University of Leipzig, Germany. Microorganism fingerprintingQuality control of a synthetic 20-mer olignucleotide, 5‘-CAA TCT GGG TGA CAG AGC AA-3‘ on the microflex (matrix: 3-hydroxypicolinic acid): The MS of the oligonucleotide reveals its exact monoisotopic mass. Oligonucleotide QC6176.10[M+H]+4000 5000 6000 7000 m/z6170 6175 61806165 m/z6177.06176.06175.06174.16173.1Dendrogram generationBiomarker AnalysisProteome profiling microflex supports the discovery of biomarker patterns and the identification of individual biomarkers in clinical pro-teomics applications. Superior system performance enables detection of biomarkers throughout the typical mass range for peptides and proteins.A complete solution for Clinical Prote-omics microflex is integrated into theCLINPROTTM suite for peptide andprotein biomarker discovery, providing automated magnetic bead based sample preparation and software tools for dataanalysis, visualization and statistical model building.Biomarker profilingSerum profiles can be observed in linear mode exceeding the typical marker range from 1-10 kDa. Additionally, the reflector mode can be used for high mass accuracy peptide marker discovery.CLINPROTmicroThe CLINPROTmicroTM is an affordable, easy-to-use and compact clinical system dedicated to peptide and protein profiling, biomarker discovery and biomarker validation. It is based on the microflex MALDI-TOF mass spectro-meter, providing excellent biomarker detection sensitivity and resolution, as well as the reliability and reproducibility needed for rigorous validation studies of putative biomarkers for cancer and other diseases. The research packageThe package is ideal for cancer research and other clinical and diagnostics research laboratories engaged in biomar-ker discovery across a range of samples. The CLINPROT workflow comprises sample prepa-ration, MALDI-TOF MS analysis, biomarker pattern detection and biomarker identification.The CLINPROTmicro solution consists of:Unique CLINPROT magnetic bead kits for facile, scaleable and highly reproducible sample preparation.Robust and easy-to-use MALDI-TOF system.Integrated CompassTM software for intuitive operation by expert and non-expert users alike.Patented AnchorChipTM MALDI target technology for fast automated acquisition and a 10–100 fold sensitivity increase.ClinProTools bioinformatics system for smart visualization of large numbers of profiles, the creation of predictive models, the validation of models via cluster analysis, and the classification of unknown samples - addressing all the major functions in biomarker pattern profiling and validation.DiseaseControlAnchorChip technologyThe MALDI (matrix-assisited laser desorption/ionization) process is a vital step for reliable analysis. The patented AnchorChipTM technology accompanies the superior instrument design being the ideal sample carrier to provide homoge-neous, exactly-positioned samples for robust and rapid automated data collec-tion, as well as a sensitivity boost by up to two orders of magnitude.Enabling DesignDesignThe modular design of the instrument offers several configurations to match a variety of application needs. Anytime, the system can be upgraded easily to meet new analytical challenges.Intuitive instrument controlThe microflex operates within our inte-grated CompassTM software environment which is used throughout our entire MS product line. All necessary functions from automated acquisition to in-depth analysis via highly sophisticated bioinfor-matics software are provided. Compass offers a seamless link with the most comprehensive bioinformatics tools in the industry.Armed with these unique attributes, themicroflex is ready for no-compromise molecular analytics and research in today’s life science world.Comprehensive supportBruker Daltonics’ comprehensive service concept includes instrument features like automated self-diagnosis routines and remote service capabilities for online support.Fast acquisition and superior sensitivity due to AnchorChip technology.microScout MALDI targets in 1/4 microtiter plate size.Integrated software packages for clinical proteomics research.Technical SpecificationsBruker Daltonics is continually improving its products and reserves the right to change specifications without notice. © BDAL 11-2012. #256045MALDI source microScout Ion Source with state-of-the-art pulsed ion extraction Target area exactly ¹/4 of industry standard microtiter plates Various 96-spot target types available for specific applications Compatible with Bruker Daltonics‘ sample preparation robotics Nitrogen laser with variable repetition rate Patented AnchorChip MALDI target technology for fast automation, increased sequence coverage and 10–100 fold sensitivity increase over stainless steel target holderCompass software suite flexControlTM module for quick and easy instrument control Fuzzy-logic based AutoXecuteTM engine for on-the-fly optimization of spectral quality flexAnalysisTM module for automated and interactive data analysis, including integration with bioinformatics tools such as MALDI Biotyper, BioToolsTM, ProteinScapeTM, ClinProToolsTM, GenoToolsTM, and search engines Optional Compass Security Pack for work in regulated environments (21CFR part 11 compliance)TOF analyzer High resolution reflectron configuration (microflex) or linear only version (microflex LT) Optional MS/MS capability with autoPSD (automated post-source decay) including pre-cursor ion selection device (microflex only) Modular design allows for future upgrades No maintenance oil-free vacuum technology WhisperModeTM erases noise pollution in the labService features Remote on-line service capability Extended self diagnostics Supplementary IQ/OQ/PV procedures availableIntegration microflex is integrated with Bruker Daltonics system solutions such as MALDI Biotyper, CLINPROTmicro and GENOLINKmicroScout MALDI targets in 1/4 microtiter plate size.Bruker Daltonik GmbH Bremen · GermanyPhone +49 (0)421-2205-0 Fax +49 (0)421-2205-103 sales@bdal.deBruker Daltonics Inc. Billerica, MA · USA Fremont, CA · USAPhone +1 (978) 663-3660 Phone +1 (510) 683-4300 Fax +1 (978) 667-5993 Fax +1 (510) 490-6586 ms-sales@bdal.com cam-sales@bruker.comwww.bruker.comFor research use only. Not for use in diagnostic procedures.