Clean Genome E. coli Chemically Competent Cell Protocol - Datasheet

Transformation efficiency is tested using pUC19 control DNA, performed in duplicate. Transformed cells are grown on LB plates containing 50 µg/mL carbenicillin. Transformation efficiency is ≥ 1x108 cfu/µg DNA for the core Clean Genome® E. coli strain, but may be lower for strains carrying mutations that reduce recombination and or mutation frequencies.