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FormThermoinactivated liquid crude cell lysateStorage buffer:50mM Tris pH7.5, 10mM CuCl2, 1%TritonX-100ApplicationsOxidation and Decomposition of various aromatic and non-aromaticcompounds by a radical-catalysed reaction mechanism, Decolorization ofdyes, Delignification of biomass, Detoxification of substrate for alcoholicfermentation, Reduction of chemical oxygen demand and phenol levelsfrom wastewater, Bioremediation.DescriptionLaccases are oxidoreductases belonging to the multinuclear copper-containing oxidases and their uses span from the textile to the pulp andpaper industries, and from food applications to bioremediation processes.Laccases also have uses in the organic synthesis, pharmaceutical andnanotechnological sectors. These oxidase enzymes catalyze oxidation ofsubstrates at the expense of molecular oxygen and produce water as theonly by-product.Laccase Data sheetEC 1.10.3.2 (Liquid form)Benzenediol oxygen oxidoreductaseLast update: March 23, 2011Batch-No10-101-UF-201008MetGen Oy | Itäinen Pitkäkatu 4B | 20520 Turku, Finland | Tel.:+358 2 4788341 | VAT# FI20755849 | http://www.metgen.comCat.-No: 10-101-UFSelected references:•Riva, S. (2006). Laccases: blue enzymes for green chemistry. Trends in biotechnology 24, 219-26.•Rodríguez Couto, S., and Toca Herrera, J. L. (2006). Industrial and biotechnological applications of laccases: a review. Biotechnology advances 24,500-13.•Shyur, L.-fen (2007). Enzymes for Pre-treatment of Lignocellulosics : Ligninases and Laccases. Biotechnology.•Skals, P. B., Krabek, A., Nielsen, P. H., and Wenzel, H. (2007). Environmental assessment of enzyme assisted processing in pulp and paper industry.The International Journal of Life Cycle Assessment 13, 124-132.Activity1,3 µkat/g at 60ºC in 100mM succinic acid pH3 with 5mM ABTS assubstrate.The activity for ABTS is calculated such as:Where:Abs (405nm) is the change in absorbance of the reaction mixtureduring the reaction time (measured in 1 cm cuvet ).38.6 x103 is molar extinction of ABTS.t (sec) is time of the reaction in secondsP(g/L) is protein concentration in the reaction in gram per litre106 is for converting Katal to microKatalTolerated reaction conditions:Temperature:+4ºC-80ºC.pH: 3-9Protein concentration:7.22 mg/mlµkat/g =Abs (405nm) x 10638.6 x 103 x t(sec) x P(g/L)For in vitro use onlyQuality guaranteed for 6 monthsStore at -80ºC, keep at +4ºC for use