Dikma - HPLC / UHPLC Columns Brochure

Dikma Technologies Inc.www.dikmatech.comHPLC | UHPLC Columns2www.dikmatech.comHPLC | UHPLC ColumnsAbout Dikma HPLC | UHPLC Columns Dikma has over 15 years of R&D experience in HPLC / UHPLC columns. Dikma HPLC / UHPLC columns have been widely used by pharmaceutical manufacturers. We offer columns of ultimate performance and maximum versatility to address the challenges and increasing needs of today's chromatographic laboratories. The quality of packing material is the basis for all good chromatographic separation. Dikma is in a unique position to control the manufacturing process from start to finish, from making high-purity (99.999%) raw silica to applying different bonding chemistries. Furthermore, to ensure the high performance and robustness of our columns, we maintain tight specifications during all stages of the manufacturing process and follow rigid, audited ISO 9001 procedures to guarantee reproducible products. The end results that get delivered to you are columns that perform at their best and offer you highly reproducible batch-to-batch results without alteration of HPLC / UHPLC conditions.Dikma HPLC | UHPLC Columns ListBrand Chemistry FeaturesEndeavorsil™ Endeavorsil™ C18• 1.8 µm UHPLC columns• Exceptional speed, resolution, and sensitivityLeapsil™ Leapsil™ C18• 2.7 µm, compatible with all HPLC and UHPLC instruments• Flexible method developmentInspire™ Inspire™ C18• High-end columns to meet high separation demands• Rapid separations with outstanding resolutions• Superior batch-to-batch reproducibilityInspire™ C8Inspire™ HILIC Inspire™ Diol Spursil™ Spursil™ C18 • Polar modification columns with maximal polar retention and selectivity• Silanol shielding for excellent peak shape• Suitable for highly aqueous mobile phase conditionsSpursil™ C18-EPPlatisil™ Platisil™ C18• Universal and cost-effective columns• Unique selectivity, excellent peak shape• Useful in normal or reversed phase applicationsPlatisil™ NH2 Platisil™ CN Platisil™ PH Platisil™ Silica Diamonsil®Diamonsil® C18• Universal reversed phase columns• High efficiency and outstanding lifetimeDiamonsil® C8Diamonsil® AAABio-Bond™ Bio-Bond™ C18• 300Å, designed to analyze and purify proteins, peptides, and biomolecules• Direct scale-up to preparative materialBio-Bond™ C8Bio-Bond™ C4NEWNEWNEWNEWNEWNEW3www.dikmatech.comHPLC | UHPLC ColumnsEndeavorsil™ Material CharacteristicsBonded phase Particle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Phase density (µmol/m2)Carbon loading (%)pH range Endcapping C18 1.8 120 300 > 99.999 3.5 20 1.5-9 Yes1.8 µm Microbore Columns (2.1 mm ID)Phase 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1Endeavorsil™ C18 87001 87002 87003 87004Endeavorsil™ Ordering Information3X Resolution 702 s183 s94 sSelectivity*Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Column: Mobile Phase: Temperature: Detection: Sample: Listed on chromatograms 50 x 2.1 mmMeCN:H2O = 60:40 0.5 mL/min Ambient UV 254 nm 1. Uracil2. Caffeine3. Phenol4. TolueneListed on chromatogramsMeCN:H2O = 55:45Ambient UV 254 nm1. Uracil2. Acetophenone3. Methyl benzoate4. Toluene5. NaphthaleneDikmaEndeavorsil™ 1.8 µm C18 (Cat.# 87002)5. Butylbenzene 6. o-Terphenyl 7. Triphenylene 8. AmylbenzeneWatersACQUITY UPLC HSS T3 1.8 µm C18AgilentZORBAX Eclipse Plus RRHD 1.8 µm C18DikmaInspire™ 5 µm C18, 150 x 4.6 mm (Cat.# 81001)Flow Rate: 1.0 mL/min DikmaInspire™ 5 µm C18, 50 x 2.1 mm (Cat.# 81003)Flow Rate: 0.3 mL/minDikmaEndeavorsil™ 1.8 µm C18, 50 x 2.1 mm(Cat.# 87002)Flow Rate: 0.5 mL/min7X faster* ACQUITY UPLC is a registered trademark of Waters Corporation. Dikma Technologies Inc. is not affiliated with the above company.* ZORBAX is a registered trademark of Agilent Technologies. Dikma Technologies Inc. is not affiliated with the above company.Features of Endeavorsil™ Columns• Combined speed, resolution, and sensitivity• Reduced analysis time and solvent waste• High efficiency combined with high selectivity and productivity• Superior column performance at higher pressure• Excellent separation characteristics over a wide pH rangeEndeavorsil™ 1.8 µm UHPLC ColumnsSpeedUnseparatedUnseparated4www.dikmatech.comHPLC | UHPLC ColumnsLeapsil™ Material CharacteristicsBonded phase Particle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Phase density (µmol/m2)Carbon loading (%)pH range Endcapping C18 2.7 100 440 > 99.999 3.9 27 1.5-10 YesLeapsil™ HPLC | UHPLCCompatible Columns Features of Leapsil™ Columns• Ultra fast separation without compromising resolution• Low operating pressure allows higher flow rates• Compatible with all HPLC and UHPLC instruments• Method development flexibility • Wide pH stability• Full spectrum of phases and selectivities1.0 2.0 Min.134 5021234 50 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 Min.1 23456781 2 3 4 5 6 7 8 9 Min.01 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Min.0123456+78Speed Selectivity* Column: Cat.No.: Mobile Phase: Temperature: Detection: Sample: Column: Dimension: Mobile Phase: Temperature: Detection: Sample: Leapsil™ 2.7 µm C18, 50 x 2.1 mm86004MeCN:H2O = 60:40 30 oCUV 254 nm1. Uracil2. Acetophenone3. Methyl benzoate4. Toluene5. NaphthaleneListed on chromatograms 50 x 2.1 mm MeOH:H2O = 45:55 30 oCUV 254 nm 1. Thiourea 2. Aniline3. Phenol 4. o, m, p-Methyl anilineFlow Rate: 0.3 mL/minFlow Rate: 1.0 mL/minHigh speed without compromising resolution5. N, N-Dimethylaniline6. Ethyl benzoate7. Toluene8. EthylbenzeneDikma Leapsil™ 2.7 µm C18 (Cat.# 86004)Flow Rate: 0.8 mL/min, Pressure: 4,945 psiAgilent ZORBAX Eclipse Plus RRHD 1.8 µm C18 Flow Rate: 0.55 mL/min, Pressure: 12,717 psiWaters ACQUITY UPLC BEH 1.7 µm C18 Flow Rate: 0.4 mL/min, Pressure: 8,897 psi57 s* ZORBAX is a registered trademark of Agilent Technologies. Dikma Technologies Inc. is not affiliated with the above company.* ACQUITY UPLC is a registered trademark of Waters Corporation. Dikma Technologies Inc. is not affiliated with the above company.Leapsil™ Ordering Information2.7 µm Microbore Columns (2.1 mm ID) Phase 50 x 2.1 100 x 2.1 150 x 2.1Leapsil™ C18 86004 86005 860062.7 µm Analytical Columns (3.0 mm ID)Phase 50 x 3.0 100 x 3.0 150 x 3.0Leapsil™ C18 86007 86008 860092.7 µm Analytical Columns (4.6 mm ID)Phase 50 x 4.6 100 x 4.6 150 x 4.6Leapsil™ C18 86001 86002 86003Unseparated5www.dikmatech.comHPLC | UHPLC ColumnsInspire™ HPLC ColumnsFeatures of Inspire™ Columns• High efficiency• Longer column lifetime• Efficient method development• Outstanding selectivity• Extreme resolution• Excellent batch-to-batch reproducibilityInspire™ Material CharacteristicsBonded phase Particle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Phase density (µmol/m2)Carbon loading (%)pH range Endcapping C18 3, 5, 10 100 440 > 99.999 3.9 27 1-11 Yes C8 3, 5, 10 100 440 > 99.999 4.2 17 1-11 Yes HILIC 3, 5, 10 100 440 > 99.999 – – 1.5-7.5 No Diol 3, 5, 10 100 440 > 99.999 2.1 7.5 2-7.5 NoTCAs at Neutral pH Conditions* Antibacterials*Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms 150 x 4.6 mmMeCN:20 mM phosphate buffer (pH 7) = 2:1 1.0 mL/min Ambient UV 254 nm 1. Desipramine 2. Nortriptyline 3. Imipramine 4. Amitriptyline 5. Trimipramine Dikma Inspire™ 5 µm C18(Cat.# 81001)Waters XBridge 5 µm C18Dikma Inspire™ 5 µm C18(Cat.# 81001)Waters XBridge 5 µm C18Listed on chromatograms 150 x 4.6 mm 0.1% HCOOH in MeCN:0.1% HCOOH in H2O = 20:801.0 mL/min Ambient UV 254 nm 1. Sulfanilamide 2. Carbadox 3. Sulfapyridine 4. Sulfamerazine 5. Thiamphenicol6. Sulfamethoxypyridazine7. Furazolidone 8. Sulfamethoxazole9. Sulfisoxazole 10. Oxolinic acid * XBridge is a trademark of Waters Corporation. Dikma Technologies Inc. is not affiliated with the above company.NEWNEW6www.dikmatech.comHPLC | UHPLC Columns3 µm Microbore Columns (2.1 mm ID) Guard Cartridges, 2/pk Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1 10 x 2.1 Inspire™ C18 81030 81004 81012 81013 81015 6501 Inspire™ C8 81130 81104 81112 81113 81115 6502 Inspire™ HILIC 81430 81404 81412 81413 81415 – Inspire™ Diol 81230 81204 81212 81213 81215 – 3 µm Analytical Columns (3.0 mm ID) Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 10 x 2.1 Inspire™ C18 81029 81021 81022 81023 81024 6501 Inspire™ C8 81129 81121 81122 81123 81124 6502 Inspire™ HILIC 81429 81421 81422 81423 81424 – Inspire™ Diol 81229 81221 81222 81223 81224 – 3 µm Analytical Columns (4.6 mm ID) Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 10 x 4.0 Inspire™ C18 81031 81016 81017 81018 81020 6601 Inspire™ C8 81131 81116 81117 81118 81120 6602 Inspire™ HILIC 81431 81416 81417 81418 81420 – Inspire™ Diol 81231 81216 81217 81218 81220 – 5 µm Microbore Columns (2.1 mm ID) Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1 10 x 2.1 Inspire™ C18 81033 81003 81007 81002 81009 6503 Inspire™ C8 81133 81103 81107 81102 81109 6504 Inspire™ HILIC 81433 81403 81407 81402 81409 – Inspire™ Diol 81233 81203 81207 81202 81209 – 5 µm Analytical Columns (3.0 mm ID) Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 10 x 2.1 Inspire™ C18 81032 81025 81026 81027 81028 6503 Inspire™ C8 81132 81125 81126 81127 81128 6504 Inspire™ HILIC 81432 81425 81426 81427 81428 – Inspire™ Diol 81232 81225 81226 81227 81228 – 5 µm Analytical Columns (4.6 mm ID) Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 10 x 4.0 Inspire™ C18 81034 81010 81011 81001 81006 6603 Inspire™ C8 81134 81110 81111 81101 81106 6604 Inspire™ HILIC 81434 81410 81411 81401 81406 – Inspire™ Diol 81234 81210 81211 81201 81247 –EasyGuard™ Guard Holder: Cat.# 6220Inspire™ Ordering Information 5 µm and 10 µm Semi-preparative Columns Guard Cartridges, 2/pk PhasesParticle Size(µm) 250 x 4.6 250 x 10.0 150 x 21.2 250 x 21.2 10 x 10.0 10 x 21.2 Inspire™ C18 5 81006 81038 81045 81039 6505 6506 Inspire™ C18 10 81035 81036 81046 81037 6511 6512 Inspire™ C8 5 81106 81138 81145 81139 6507 6508 Inspire™ C8 10 81135 81136 81146 81137 6513 6514 Inspire™ HILIC 5 81406 81438 81445 81439 – – Inspire™ HILIC 10 81435 81436 81446 81437 – – Inspire™ Diol 5 81247 81238 81245 81239 6509 6510 Inspire™ Diol 10 81235 81236 81246 81237 6515 651610 mm Guard Holder: Cat.# 6221; 21.2 mm Guard Holder: Cat.# 62227www.dikmatech.comHPLC | UHPLC ColumnsSpursil™ HPLC ColumnsFeatures of Spursil™ Columns• Combine high-purity silica with unique polar modification technology• Maintain characteristics of conventional reversed phases• Unique selectivity and enhanced resolution• Reduced silanol interactions and improved peak shape for basic analytes• Stable retention in highly aqueous mobile phase conditions• Enhanced retention for polar compounds• Extended range pH stability• Choose from a variety of selectivities and hardware formatsSpursil™ Material CharacteristicsBonded phase Particle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Phase density (µmol/m2)Carbon loading (%) pH range EndcappingC18 3, 5, 10 100 440 > 99.999 3.5 25 1.5-10 YesC18-EP 3, 5, 10 100 440 > 99.999 3.4 24 1.5-10 Yes1+21+234Min.Min.Min.Column: Cat.No.: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample:Column:Dimension:Mobile Phase:Flow Rate:Temperature:Detection: Sample:* Inertsil is a registered trademark of GL Sciences Inc.. Dikma Technologies Inc. is not affiliated with the above company.* Luna is a registered trademark of Phenomenex. Dikma Technologies Inc. is not affiliated with the above company.* ZORBAX is a registered trademark of Agilent Technologies. Dikma Technologies Inc. is not affiliated with the above company.* XBridge is a trademark of Waters Corporation. Dikma Technologies Inc. is not affiliated with the above company.Cephalosporin Polar Compounds* Steric Selectivity*Spursil™ 5 µm C18, 150 x 4.6 mm82001MeOH:0.1% TFA in H2O = 30:701.0 mL/min Ambient UV 230 nm 1. Ceftazidime 2. Cefadroxil 3. Cefuroxime Listed on chromatograms 150 x 4.6 mm10 mM HCOONH4 (pH 3) 1.0 mL/min Ambient UV 254 nm 1. Cytosine 2. Thiourea 3. Adenine 4. Uridine 4. Cefazoline 5. Cefaclor 6. Cephalexin DikmaSpursil™ 5 µm C18-EP(Cat.# 82101)Phenomenex Luna 5 µm C18(2)GL SciencesInertsil 5 µm ODS-3AgilentZORBAX5 µm Bonus-RPWatersXBridge 5 µm C18injectionAfter 500 injectionsAfter 1,000 injections1st1+21+234Min.Min.Min.DikmaSpursil™ 5 µm C18-EP(Cat.# 82101)Listed on chromatograms150 x 4.6 mmMeOH:H2O = 80:20 1.0 mL/min Ambient UV 254 nm 1. N,N-Dimethylaniline 2. Ethylbenzene 3. Propylbenzene 4. Bibenzyl 5. trans-Stilbene Spursil™ Ordering Information3 µm Microbore Columns (2.1 mm ID) 5 µm Microbore Columns (2.1 mm ID)Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1Spursil™ C18 82030 82004 82012 82013 82015 82033 82003 82007 82002 82009Spursil™ C18-EP 82130 82104 82112 82113 82115 82133 82103 82107 82102 821093 µm Analytical Columns (3.0 mm ID) 5 µm Analytical Columns (3.0 mm ID)Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0Spursil™ C18 82029 82021 82022 82023 82024 82032 82025 82026 82027 82028Spursil™ C18-EP 82129 82121 82122 82123 82124 82132 82125 82126 82127 821283 µm Analytical Columns (4.6 mm ID) 5 µm Analytical Columns (4.6 mm ID)Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6Spursil™ C18 82031 82016 82017 82018 82020 82034 82010 82011 82001 82006Spursil™ C18-EP 82131 82116 82117 82118 82120 82134 82110 82111 82101 82106EasyGuard™ Guard Holder: Cat.# 62208www.dikmatech.comHPLC | UHPLC ColumnsPlatisil™ Material CharacteristicsPlatisil™ HPLC ColumnsFeatures of Platisil™ Columns• Universal and cost-effective columns• Unique selectivity, excellent peak shape• Useful in normal or reversed phase applications• High loadabilityBonded phase Particle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Phase density (µmol/m2)Carbon loading (%)pH range Endcapping C18 5 100 440 > 99.999 1.8 15 1-11 Yes NH2 5 100 440 > 99.999 3.2 7 2-7.5 No CN 5 100 440 > 99.999 4.8 12 1.5-7.5 Yes Silica 5 100 440 > 99.999 - - 1.5-7.5 No PH 5 100 440 > 99.999 4.1 14 1.5-7.5 YesPlatisil™ Ordering Information5 µm Analytical ColumnsPhases 150 x 4.6 250 x 4.6Platisil™ C18 99501 99503Platisil™ NH2 99504 99505Platisil™ CN 99506 99507Platisil™ Silica 99508 99509Platisil™ PH 99510 99511Platisil™ 5 µm C18, 250 × 4.6 mm99503MeOH:0.1% phosphoric acid = 80:201.0 mL/minUV 254 nm30 oC1. Aloe emodin2. Rhein3. Emodin4. Chrysophanol5. PhyscionColumn: Cat. No.:Mobile Phase: Flow Rate: Detection: Temperature: Sample:Platisil™ 5 µm C18, 150 × 4.6 mm99501MeOH:MeCN:1% sodium dodecyl sulfate:acetic acid=30:35:30:0.31.0 mL/minUV 265 nmAmbient1. Pseudoephedrine hydrochloride2. Naphazoline hydrochloride3. Chlorpheniramine4. Dextromethorphan hydrochlorideColumn: Cat. No.:Mobile Phase: Flow Rate: Detection: Temperature: Sample:Cold Medicine The Active Ingredients of RhubarbNEWNEWNEWNEW0 10 20 30 Min.12 34520 Min.0 1510512349www.dikmatech.comHPLC | UHPLC ColumnsDiamonsil® Material CharacteristicsDiamonsil® HPLC ColumnsFeatures of Diamonsil® Columns• Universal reversed phase columns• High efficiency and outstanding lifetime• Suitable for faster method development• Superior batch-to-batch reproducibilityBonded phase Particle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Impurities (mg/kg)Carbon loading (%)pH range Endcapping C18(2) 3, 5, 10 100 440 > 99.999 < 10 27 1.5-9 Yes C8(2) 3, 5, 10 100 440 > 99.999 < 10 17 1.5-9 Yes C18 5 100 440 > 99.999 < 10 17 2-7.5 Yes C8 5 100 440 > 99.999 < 10 10 2-7.5 Yesß-Blockers at High pH* Water-Soluble Vitamins*Listed on chromatograms150 × 4.6 mmMeOH:5 mM NH4HCO3 (pH 10) = 70:301.0 mL/minAmbientUV 220 nm1. Pindolol2. Acebutolol3. Metoprolol4. Bisoprolol5. Propranolol6. AlprenololColumn: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms150 × 4.6 mmMeOH:10 mM HCOONH4 (pH 3) = 5:951.0 mL/minAmbientUV 254 nm1. Pyridoxamine2. Orotic acid3. L-Ascorbic acid4. Pyridoxal5. Pyridoxol6. Nicotinamide*Luna is a registered trademark of Phenomenex. Dikma Technologies Inc. is not affiliated with the above company.*Symmetry is a registered trademark of Waters Corporation. Dikma Technologies Inc. is not affiliated with the above company.*HC is a trademark of Agilent Technologies. Dikma Technologies Inc. is not affiliated with the above company.DikmaDiamonsil® 5 µm C18(2)(Cat.# 99601)DikmaDiamonsil® 5 µm C18(2)(Cat.# 99601)PhenomenexLuna 5 µm C18WatersSymmetry 5 µm C18WatersSymmetry 5 µm C18Agilent5 µm HC-C18UnseparatedUnseparatedUnseparatedPoor ResolutionPoor Resolution0 2 4 6 8 Min.1234560 2 4 6 8 10 Min.1234560 2 4 6 8 Min.12345 61 2 3 4 Min.1 234 5601 2 3 4 5 Min.123456012+34 561 2 3 4 5 Min.012+3 4561 2 3 4 5 Min.013+45+261 2 3 4 5 Min.01 2 3 4 Min.1 234 5601 2 3 4 5 Min.123456012+34 561 2 3 4 5 Min.012+3 4561 2 3 4 5 Min.013+45+261 2 3 4 5 Min.01 2 3 4 Min.1 234 5601 2 3 4 5 Min.123456012+34 561 2 3 4 5 Min.012+3 4561 2 3 4 5 Min.013+45+261 2 3 4 5 Min.010www.dikmatech.comHPLC | UHPLC ColumnsDiamonsil® Ordering Information 3 µm Microbore Columns (2.1 mm ID) Guard Cartridges, 2/pk Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1 10 x 2.1Diamonsil® C18(2) 99631 99611 99612 99613 99615 6311Diamonsil® C8(2) 99681 99661 99662 99663 99665 6312 3 µm Analytical Columns (3.0 mm ID) Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 10 x 2.1Diamonsil® C18(2) 99632 99621 99622 99623 99625 6311Diamonsil® C8(2) 99682 99671 99672 99673 99675 6312 3 µm Analytical Columns (4.6 mm ID) Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 200 x 4.6 250 x 4.6 10 x 4.0Diamonsil® C18(2) 99633 99616 99617 99618 99619 99620 6331Diamonsil® C8(2) 99683 99666 99667 99668 99669 99670 6332 5 µm Microbore Columns (2.1 mm ID) Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1 10 x 2.1Diamonsil® C18(2) 99634 99604 99605 99606 99608 6313Diamonsil® C8(2) 99684 99654 99655 99656 99658 6314 5 µm Analytical Columns (3.0 mm ID) Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 10 x 2.1Diamonsil® C18(2) 99635 99626 99627 99628 99630 6313Diamonsil® C8(2) 99685 99676 99677 99678 99680 6314 5 µm Analytical Columns (4.6 mm ID) Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 200 x 4.6 250 x 4.6 10 x 4.0Diamonsil® C18(2) 99636 99609 99610 99601 99602 99603 6333Diamonsil® C8(2) 99686 99659 99660 99650 99652 99651 6334Diamonsil® C18 - - - 99901 99902 99903 6201Diamonsil® C8 - - - 99801 - 99803 6202EasyGuard™ Guard Holder: Cat.# 62205 µm and 10 µm Semi-preparative Columns Guard Cartridges, 2/pkPhases Particle Size(µm) 250 x 4.6 250 x 10.0 150 x 21.2 250 x 21.2 10 x 10.0 10 x 21.2Diamonsil® C18(2) 5 99603 99644 99770 99654 6335 6336Diamonsil® C8(2) 5 99651 99694 99771 99695 6339 6340Diamonsil® Diol 5 99753 99754 99772 99755 6343 6344Diamonsil® Silica 5 - 99724 99773 99725 6347 6348Diamonsil® C18(2) 10 99641 99642 99774 99643 6337 6338Diamonsil® C8(2) 10 99691 99692 99775 99693 6341 6342Diamonsil® Diol 10 99761 99762 99776 99763 6345 6346Diamonsil® Silica 10 99721 99722 99777 99723 6349 635010 mm Guard Holder: Cat. #6221; 21.2 mm Guard Holder: Cat.# 6222Amino Acid Analysis (AAA) ColumnPhases Particle Size (µm) 250 × 4.6Diamonsil® AAA 5 9975111www.dikmatech.comHPLC | UHPLC ColumnsInsulin Genetic Variant Chemotactic PeptideColumn: Cat. No.:Mobile Phase:Flow Rate: Detection: Sample: 1. Bovine Insulin2. Human Insulin3. Porcine InsulinBio-Bond™ 5 µm C18, 250 x 4.6 mm84006A: 0.1% TFA in H2OB: 0.1% TFA in MeCNA:B = 70:30 to A:B = 65:35 in 25 min1.0 mL/min UV 210 nmColumn: Cat. No.:Mobile Phase: Flow Rate: Detection: Sample: Bio-Bond™ 5 µm C4, 250 x 4.6 mm84406A: 0.1% TFA in H2OB: 0.1% TFA in MeCN:0.1% TFA in H2O = 90:10A:B = 60:40 to A:B = 24:76 in 18 min1.0 mL/min UV 210 nm1. N-Formyl-Nle-Leu-Phe-Nle-Tyr-Lys 2. Lle-Val-Pro-Phe-Lyl-Pro-Leu-Thr-Amide3 µm Analytical Columns Guard Cartridges, 2/pkFor 2.1 ID Column For 4.6 ID Column Phases 50 x 2.1 150 x 2.1 50 x 4.6 150 x 4.6 250 x 4.6 10 x 2.1 10 x 4.0 Bio-Bond™ C18 84004 84013 84016 84018 84020 6901 6951 Bio-Bond™ C8 84104 84113 84116 84118 84120 6902 6952 Bio-Bond™ C4 84404 84413 84416 84418 84420 6905 69555 µm Analytical Columns Phases 50 x 2.1 150 x 2.1 50 x 4.6 150 x 4.6 250 x 4.6 10 x 2.1 10 x 4.0 Bio-Bond™ C18 84003 84002 84010 84001 84006 6903 6953 Bio-Bond™ C8 84103 84102 84110 84101 84106 6904 6954 Bio-Bond™ C4 84403 84402 84410 84401 84406 6906 6956EasyGuard™ Guard Holder: Cat.# 6220 5 µm & 10 µm Semi-preparative Columns Phases Particle Size (µm) 250 x 4.6 250 x 10.0 150 x 21.2 250 x 21.2 10 x 10.0 10 x 21.2 Bio-Bond™ C18 5 84006 84038 84045 84039 6907 6908 Bio-Bond™ C8 5 84106 84138 84145 84139 6909 6910 Bio-Bond™ C4 5 84406 84438 84445 84439 6911 6912 Bio-Bond™ C18 10 84035 84036 84046 84037 6913 6914 Bio-Bond™ C8 10 84135 84136 84146 84137 6915 6916 Bio-Bond™ C4 10 84435 84436 84446 84437 6917 6918 10 mm Guard Holder: Cat.# 6221; 21.2 mm Guard Holder: Cat.# 6222 Bio-Bond™ Ordering InformationBio-Bond™ Material CharacteristicsBio-Bond™ Protein and Peptide Separation ColumnsFeatures of Bio-Bond™ Columns • Designed to analyze and purify proteins, peptides, and biomolecules• Available with C18, C8 and C4 bonded phases• Direct scale-up to preparative material• Outstanding reproducibility, efficiency, and column lifetimeBonded phaseParticle size(µm)Pore size(Å)Surface area (m2/g)Purity(%)Phase density (µmol/m2)Carbon loading (%)pH range EndcappingC18 3, 5, 10 300 100 > 99.999 3.7 8 2-8 YesC8 3, 5, 10 300 100 > 99.999 4.5 5 2-8 YesC4 3, 5, 10 300 100 > 99.999 4.4 3 2-8 YesAbout DikmaQualityValueDikma Technologies Inc., established in 1993, is a global technology leader committed to developing novel separation and purification solutions for life sciences and related industries. Our core technology portfolio includes products for liquid chromatography, gas chromatography, sample preparation, and bulk purification chromatographic media. We also provide related chromatography accessories. Dikma Technologies Inc. is an ISO 9001:2000 Standard Quality Assessed Company to ensure the quality and reliability of our products and services. We are dedicated to the highest standards of production, quality assurance and quality control.Dikma Technologies Inc. is committed to bringing maximum value to its customers.USA11 Orchard Road, Suite 106Lake Forest, CA 92630, USA Tel: 949-716-5810 Fax: 949-716-5811Toll-Free: 1-877-328-8348 E-mail: sale@dikmatech.comCanada40 Vogell Road, Unit 57Richmond Hill, ON L4B 3N6, CanadaTel: 905-737-8066Fax: 905-770-0181Toll-Free: 1-866-889-9072E-mail: sales@dimaglass.comwww.dikmatech.com | www.dimaglass.com©2013 Dikma Technologies Inc.Literature No. 3024