Dikma - New HPLC Columns-2012 Brochure

Dikma New HPLC ColumnsDikma Technologies Inc.www.dikmatech.com | www.dimaglass.com“Surpassing Competitor's HPLC & UHPLC Columns”“Surpassing Competitor's HPLC & UHPLC Columns”“Surpassing Competitor's HPLC & UHPLC Columns”“Surpassing Competitor's HPLC & UHPLC Columns”“Surpassing Competitor's HPLC & UHPLC Columns”SAMPLE PREPARATIONBULK PURIFICATIONCHROMATOGRAPHIC MEDIALIQUID CHROMATOGRAPHYGAS CHROMATOGRAPHYTMInspire HILIC HILIC is normal phase chromatography of polar and ionic compounds under RP conditions. It combines the characteristics of three methods in LC-RP, NP, and IC. Inspire HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of polar compounds onto the stationary phase for increased retention. Separation is achieved through the partitioning of polar solutes from the high concentration, water-miscible, organic mobile phase into the hydrophilic surface environment. Polar solutes exhibit increased retention, and elute in the order of increasing hydrophilicity. HILIC is ideal for separating and retaining polar compounds that may not retain on traditional RP packings. Unlike reversed-phase columns, Inspire HILIC columns retain highly polar compounds with only small amounts of water in the mobile phase. These more volatile mobile phases increase sensitivity with microbore applications.1Unique selectivity and increased retention for highly polar compounds1Increased sensitivity and lower detection limits with microbore1Enhanced retention for hydrophilic/polar compounds1Increased laboratory throughput and productivity1Superior batch-to-batch reproducibility1Suitable for the separation of hydrophilic compounds such as polar acids and bases, polar compounds, nucleosides, oligonucleotides, amino acids, peptides, and water-soluble vitaminsTMInspire HILIC Material CharacteristicsBonded phase Particle size(ìm)Surface area 2(m /g)Purity(%)Carbon loading (%)pH range EndcappingPore size(A)HILIC 3,5,10 100 440 > 99.999 - 1.5-7.5 NoCaffeine metabolitesColumn: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms150 × 4.6 mm, 5 µmMeCN:10 mM ammonium formate (pH=3.0) = 95:51.0 mL/minAmbientUV 254 nm1. Theophyline2. 3-Methylxanthine3. 7-Methylxanthine4. 1, 3-Dimethyluric acidCompetitor P HILICDikma Inspire HILICInspire DiolTM Inspire Diol columns are suitable for RP, NP, and HILIC modes. They are less polar than unmodified silica and very easily wettable with water. The water-enriched layer on the surface facilitates the retention of polar compounds. A well-known use of Diol columns, under normal phase conditions, is the separation of steroids and sterols.1Monomerically bonded dihydroxypropyl group1Ultra pure silica and unique bonding chemistry promote long column lifetime, excellent column reproducibility and high inertness without endcapping1Unique selectivity for polar/hydrophilic compounds in RP, NP, and HILIC modes1Lower polarity than unmodified silica and very easily wettable with water1Higher retentivity than silica in NP mode and useful tool as preparative column owing to easy dry-upInspire Diol Material CharacteristicsTM Bonded phase Particle size(ìm)Surface area 2(m /g)Purity(%)Carbon loading (%)pH range EndcappingPore size(A)3,5,10 100 440 > 99.999 2.0-7.5 No2.1 7.5DiolSteroidsColumn: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms150 4.6 mm, 5 m × µA:Hexane B:CH2Cl2:MeOH = 80:20 A:B = 80:202.0 mL/minAmbientUV 254 nm1. 11-Ketoprogesterone2. Progesterone3. Cortisone 21-acetate 4. Corticosterone5. Prednisolone 21-acetate6. Cortisone7. Prednisone8. Hydrocortisone9. Dexamethasone10. PrednisoloneDikma Inspire DiolTMInspire TMInspire -Competitor G Sil-100APhase density 2(mol/m )Phase density 2(mol/m )Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms150 4.6 mm, 5 m × µA:Hexane B:CH2Cl2:MeOH = 80:20 A:B = 95:51.0 mL/minAmbient1. Di-n-octyl phthalate2. Dibutyl phthalate3. Dipropyl phthalate4. Diethyl phthalateUV 254 nm 5. Dimethyl phthalatePlatisil NH2TM Platisil NH columns retain hydrogen-bonding compounds under three 2separation modes-RP, NP, and IC. Platisil NH columns provide reproducible 2retention and selectivity with improved column lifetime.1Aminopropyl modified silica phase for multi-mode chromatography (RP, NP, & IC)1Improved phase ruggedness and stability 1Excellent retention for polar compounds such as sugars, oligosaccharides, sugar alcohols, and other hydroxyl compounds, as well as DNA bases under RP conditions and vitamins A & D and hydrocarbons in the petroleum industry under NP conditionsPlatisil NH Material Characteristics2TM Bonded phase Particle size(ìm)Surface area 2(m /g)Purity(%)Carbon loading (%)pH range EndcappingPore size(A)NH23,5,10 100 440 > 99.999 2.0-7.5 No3.2 7Water-soluble vitaminsColumn: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms150 4.6 mm, 5 m × µMeCN:25 mM KH2PO4(pH=2.5) = 70:301.0 mL/minAmbientUV 254 nm1. Riboflavin2. Nicotinamide3. Pyridoxine4. Thiamine Competitor P NH2Dikma Platisil NH2Platisil CN columns offer a unique polar selectivity in RP and NP mode. They provide sharp peaks and great reproducibility run-to-run, column-to-column, and batch-to-batch. The smooth silica allows for a more uniform bonding with improved resistance to bonded phase hydrolysis to produce one of the most stable CN phases. The high coverage combined with a thorough endcapping makes Platisil CN columns suitable for the separation of ionizable compounds such as basic drugs, organic acids, and steroids as well as carboxyl, carbonyl, and amine containing compounds. TMPlatisil CN11Exceptionally high surface coverage and alternative selectivity1Low hydrophobicity for rapid elution of hydrophobic analytes1More reproducible separations than silica for NP applications1Multi-mode column (RP, NP, & Hilic) widens scope of selectivity1High retention capacity for polar and unsaturated compounds1Excellent reproducibility and superior stability1Quick equilibration and less sensitivity to small changes of the water content in the mobile phase1Suitable for the separation of ionizable compounds such as basic drugs, organic acids, and steroidsMonomerically bonded cyanopropyl groupBonded phase Particle size(ìm)Surface area 2(m /g)Purity(%)Phase density 2(mol/m )Carbon loading (%)pH range EndcappingPore size(A)TMPlatisil CN Material CharacteristicsSelectivity and retention comparisonCN 3,5,10 100 440 > 99.999 1.5-7.5 Yes4.8 12Dikma Platisil CN Dikma Inspire C18Column: Dimension: Mobile Phase: Flow Rate: Temperature: Detection: Sample: Listed on chromatograms150 4.6 mm, 5 m × µMeCN:H2O =65:351.0 mL/minAmbient1. Uracil2. Caffeine 3. Phenol4. Toluene5. Butylbenzene6. Amylbenzene7. o-Terphenyl8. TriphenyleneUV 254 nm Competitor G Sil-100ADikma Platisil SilicaPlatisil SilicaTM Platisil Silica is ideal for the rapid separation of low molecular weight compounds that are soluble in organic solvents. Separation on silica columns depend upon the difference in orientation, type, and number of functional groups associated with the compounds in the sample.1High loading capacity and strong mechanical stability due to exceptionally stable silica packing1Minimal peak distortion1Useful for separating compounds differing in the number and type of the functional groups1Suitable for the separation of stereoisomers as well as neutral and weakly acidic compounds1Excellent batch-to-batch reproducibilityPlatisil Silica Material CharacteristicsTM Bonded phase Particle size(ìm)Surface area 2(m /g)Purity(%)Carbon loading (%)pH range EndcappingPore size(A)Silica 3,5,10 100 440 > 99.999 1.5-7.5 No-Phthalate estersTMPlatisil TMPlatisil Phase density 2(mol/m )Phases 30 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0TMInspire Ordering InformationPhases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6Phases Phases Phases 30 x 2.130 x 3.030 x 4.650 x 2.150 x 3.050 x 4.6100 x 2.1100 x 3.0100 x 4.6150 x 2.1150 x 3.0150 x 4.6250 x 2.1250 x 3.0250 x 4.650 x 3.0Phases 250 x 4.6 250 x 10.0 150 x 21.2 250 x 21.23 µm Microbore Columns (2.1 mm)3 µm Analytical Columns (3.0 mm) 3 m Analytical Columns (4.6 mm)µ5 m Microbore Columns (2.1 mm)µ5 m Analytical Columns (3.0 mm)µ5 m Analytical Columns (4.6 mm)µ5 m & 10 m Semi-preparative Columnsµ µTM Inspire HilicTM Inspire Diol TM Inspire HilicTM Inspire Diol TM Inspire HilicTM Inspire Diol TM Inspire HilicTM Inspire Diol TM Inspire HilicTM Inspire Diol TM Inspire HilicTM Inspire Diol 8143081230814048120481412812128141381213814158121581429 81421 81422 81423 8142481229 81221 81222 81223 8122481431 81416 81417 81418 8142081231 81216 81217 81218 8122081433 81403 81407 81402 8140981233 81203 81207 81202 8120981432 81425 81426 81427 8142881232 81225 81226 81227 8122881434 81410 81411 81401 8140681234 81210 81211 81201 81247Particle Size ( m)µTMInspire HilicTMInspire HilicTMInspire Diol TMInspire Diol 51051081406814358124781235814388143681238812368144581446812458124681439814378123981237TMPlatisil Ordering Information5 m Analytical ColumnsµTM Platisil NH2TM Platisil CNTM Platisil SilicaPhase 150 x 4.6995049950699508250 x 4.6995059950799509USA11 Orchard Road, Suite 106Lake Forest, CA 92630, USA Tel: 949-716-5810 Fax: 949-716-5811Toll-Free: 1-877-328-8348 Email: sale@dikmatech.comCanada40 Vogell Road, Unit 57Richmond Hill, ON L4B 3N6, CanadaTel: 905-737-8066Fax: 905-770-0181Toll-Free: 1-866-889-9072Email: sales@dimaglass.com www.dikmatech.com www.dimaglass.com | Dikma Technologies Inc. Dima Glass Inc.Literature No. 3025Dikma Technologies Inc. 1.pdf 23.pdf 4.pdf