DSD-DNPH Passive Sampling Device: Product Flyer

sigma-aldrich.comNew DSD-DNPH Passive Sampling DeviceHigh Efficiency Diffusive Sampler for the Determination of Aldehydes and Ketones in Indoor and Ambient AirBenefits l Specified in OSHA Method 1007 for Determination of Aldehydes l Fast sampling rates due to radial design l High capacity – up to 7 days (not recommended for STEL) l Stable blank data – important for LOQ l Easy extraction relative to badges and other passive samplers l Stable for wind, temperature and humidityComparison to Active SamplingExperimental ConditionsLocation: Samples from both indoor and outdoor atmospheres.Active Sampling Method:A commonly used active DNPH cartridge was used at 100 mL/min for 24 hours. A mass flow controller was used to accurately achieve 100 mL/min sampling rate. A wet-type gas meter was used to verify the sample volume.DSD-DNPH: The DSD-DNPH Sampler was used for 24 hours in the same location as the active sampler.The effective sampling rate of 71.9 mL/min of formaldehyde was obtained from the slope of the graph. The formaldehyde results and Graham’s Law were used to calculate the sampling rates for other compounds. 30 40 50 60 70 80 90 Humidity (%) Collection Amount of HCHO/ (µg)201510500 1 2 3 4 5 Wind Velocity (m/s) 86420Wind Velocity (m/s) HCHO Concentration: 40 ppb Temp.: 25 °C Humidity: 60%0 10 20 30 40 Temperature (°C) 100806040200Collection Efficiency of HCHO/HCHO Concentration (µg/ppm) HCHO Concentration: 100 ppb Temp.: 25 °C HCHO Concentration: 100 ppb Temp.: 25 °CDSD-DNPH Passive Sampling DeviceE0009620 5 10 15 20 DSD-DNPH (µg/d)302520151050Pump (µg/d)Formaldehyde y = 1.39x R2 = 0.992sigma-aldrich.comReaction Principle Carbonyl compounds like formaldehyde and acetone pass through the diffusive membrane and reacts with 2,4-dinitro-phenylhydrazine (DNPH) coated silica gel to produce a hydrazone derivative. This derivative is eluted by acetonitrile, then analyzed by HPLC or GC.Reference: S. Uchiyama and S Hasegawa, “A Reactive Sensitive Diffusion Sampler for the Determination of Aldehydes and Ketones in Ambient Air”, Atmospheric Environment 1999, 33, 1999-2005.Easy Extraction Method 1. Tap cap-side of sampler on table so the DNPH coated silica falls into the elution tube. 2. Unscrew and remove the storage tube. Important: maintain a vertical orientation with cap on the bottom. 3. Remove the cap and diffusive membrane. Important: maintain vertical orientation of elution tube. 4. Connect 6 mL reservoir to top of the elution tube using female adapter (28224-U).General Description of Elution ProcedureSpecifications Adsorbent: 2,4-DNPH impregnated silica gel, 250 mg Particle Size: 105-210 µm, spherical silica gel DNPH Amount: 1 mg per tube Capacity: 150 µg formaldehyde Background: Formaldehyde < 0.1 µg Acetaldehyde < 0.1 µg Acetone < 0.5 µg Ordering InformationDescription Qty. Cat. No.DSD-DNPH 10 28221-UPerforated Holder 10 28222-UFemale Luer Fitting to 5/32” tubing 20 28224-ULapel Clip 10 21019-UFiltration Column w/o Frit 6 mL 30 57242Plastic Color-Coded Cap Insert 100 000J004Visiprep™-DL Vacuum Manifold 1 57044Visi-1™ Sample Processor 1 57080-UCalibration StandardsTO11/IP-6A Aldehyde/Ketone – DNPH Mix 1 47285-UFormaldehyde-DNPH, 1 mL 1 47177Acetaldehyde-DNPH, 1000 µg/mL, 1 mL 1 47340-UAcetone-DNPH, 1000 µg/mL, 1 mL 1 47341Acrolein-DNPH, 1000 µg/mL, 1 mL 1 47342Propionaldehyde-DNPH, 1000 µg/mL, 1 mL 1 47181Analytical ColumnDiscovery® RP-Amide C16, 25 cm x 4.6 mm I.D., 5 µm particles 505064TRADEMARKSDiscovery, Visi-1, Visiprep - Sigma-Aldrich Biotechnology LP1 2 3 4E000963World Headquarters3050 Spruce St., St. Louis, MO 63103(314) 771-5765sigma-aldrich.comOrder/Customer Service (800) 325-3010 • Fax (800) 325-5052Technical Service (800) 325-5832 • sigma-aldrich.com/techserviceDevelopment/Bulk Manufacturing Inquiries (800) 244-1173©2008 Sigma-Aldrich Co. All rights reserved. SIGMA, , SAFC, , SIGMA-ALDRICH, ALDRICH, , FLUKA, , and SUPELCO, are trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. KIXT408065