ems Sampling Guide

Environmental Monitoring Systems, Inc. 164 Ashley Avenue Charleston, South Carolina 29403 TEL 800-293-3003 or 843-724-5708 FAX 866-724-5700 Email: info@emssales.net Website www.emssales.net Inspecting Residential Structures for Mold Contamination IESO 2210 Non-Viable (non-culturable) Direct Examination Samples for Fungal Analysis Sample Type Field Equipment(1) Recommended (1) Sampling Rate Media(1,3) Efficiency Cut off d50 Units(2) Method Reference Air Sample All Pumps Calibrated Allergenco-D Cassette High Volume Pump Up to 10 min.@ 15 lpm Allergenco-D Cassette 1.8 um Spores/m3 IESO 1210 Cyclex-d Cassette High Volume Pump Range 1-5 min. @ 20 lpm Cyclex-d Cassette 0.8 um Spores/m3 IESO 1210 Micro-5 Cassette Low Volume Pump 5 min. @ 5 lpm Micro-5 Cassette 0.8 um Spores/m3 IESO 1210 Zefon: Air-O-Cell Cassette High Volume Pump Up to 10 min.@ 15 lpm Air-O-Cell Cassette 2.6 um Spores/m3 IESO 1210 Integral Pump + Holder + Slide Allergenco – Integral Pump Programmable 10 min.@ 15 lpm Multiple Samples - Adhesive Slide 2.0 um Spores/m3 IESO 1210 Holder + Slide Burkhard – Integral Pump Programable 1-9 min @ 10 lpm Greased/Adhesive Slides 2.6 or 5.2 um Spores/m3 IESO 1210 Holder + Slide Cyclex – Slide Holder – High Volume Pump Cyclex 1-5 min. @ 20 lpm Cyclex-Adhesive Slide 1.8 um Spores/m3 IESO 1210 Wall High or Low Vol. Pump Drill, Wall-Kit 0.5-5 min. @ appropriate flow rate Air-O-Cel, Allergenco-D, Cyclex-d, Micro-5 Cassettes & Cyclex Spores/m3 Bulk/Vacuum/Tape Bulk Sealed Container, e.g. Centrifuge Tube, Plastic Bag, etc. ~ 2 square inches NA ID Vacuum High Volume Pump Highest Set Volume Known Area ~(20 ft2) or ~15 mg Total Dust Filter, MCE or PVC Cassette ID + ND, Light, Medium, Heavy, Excess IESO 1310 Tape Lift Tape Kit, Tape, Microscope Slide, Slide Holder, Plastic Bag ~1 cm2 Microscope Slide ID + ND, Light, Medium, Heavy, Excess IESO 1110 Viable (Culturable), then Direct Examination Samples for Fungal Analysis Sample Type Field Equipment Sampling Rate/Area Media Units Method Reference Air Sample All Pumps Calibrated Efficiency Cut off d50 Units(2) Air High Volume Pump, Sampler, Agar Plates Andersen Type e.g. N6 or E6 up to 10 min. @ 28.3 lpm CEL, CMA, DG18, MEA Agar 0.65-0.70 µm CFU/m3 IESO 1220 Reuter Centrifugal sampler (RCS) RCS - centrifugal impactors 20 sec-8 min. @ ca. 40 litre/min 3.8 µm CFU/m3 Bulk/Vacuum/Swab Bulk Sterile Sealed Container, Plastic Bag, etc. ~ 2 square inches NA ID Vacuum High Volume Pump Highest Set Volume Known Area or ~15 mg Total Dust MCE or PVC Cassette CFU/in2 or CFU/m3 Swab Sterile Swab Kit ~1-4 square inches Sterile Moist Swab ID + ND, Light, Medium, Heavy, Excess IESO 1120 1) Please research and use manufacturer’s recommended procedures Limited or extended sampling times are often recommended for highly contaminated or very clean environments, respectively. 2) NA = Not Applicable; ID = Identity; ND = Non-Detected; Light, Medium, Heavy, Excess = Levels of Deposited Particles 3) CEL=Cellulose Agar – Molds on Sheetrock & Wood Substrates, CMA=Corn Meal Agar – Dermatophytes and Cellulosic Fungi (Not Common in IAQ), DG18 Agar – Dichloran 18% Glycerol Agar – Xerophilic (low water activity) Fungi in Carpets, etc., MEA Agar – Malt Extract Agar –Versatile All Purpose Media (medium to high water activity).