Human IG RNA Multiplex Kit - Manual

The HUMAN IG RNA MULTIPLEX kit is designed for the amplification of IG heavy and light chain cDNA libraries using the combination of highly sensitive multiplex PCR and Unique Molecular Identifiers (UMIs). UMIs are introduced along with 1st strand cDNA synthesis and allow for error-free reconstruction of full-length IG heavy and light chain sequence with hypermutations (except for the FR1 region). In addition, UMIs allow for exact quantification of template cDNA molecules, control for input bottlenecks, and accurate normalization of samples of comparison. Gene-specific primers targeting IG heavy chain constant regions facilitate all IGH isotype discrimination including IgM, IgD, IgG3, IgG1, IgA1, IgG2, IgG4, IgE, and IgA2.