LEAP - ITEX2 Option - In-Tube Extraction for GC-PAL - Brochure

ITEX-2 OptionPrep and Load PlatformHigh Sensitivity Enrichment Technique for Gas Chromatography Environmental / Drinking WaterFood / Flavour / Consumer ProductsForensics / ToxicologyPetrochemicals / Polymers Pharmaceuticals / Residual SolventsPart No. PAL Broch-ITEX-2-10CTC Analytics acknowledges all tradenames and trademarks used as the property of their respective ownersCTC Analytics AGSwitzerlandinfo@ctc.chwww.palsystem.comCTC AnalyticsWhere design meets performancePAL is a registered trademark of CTC Analytics AG | Switzerland CTC Analytic's aim is to supply instruments to customers which make the operation of sample processing simple and transparent. In-line with todays lab requirements for productivity, CTC expanded the application range of it's GC Injector System PAL Combi-xt introducing the ITEX-2 Option. The ITEX-2 Option consists of an add-on module which can be used with any existing or new PAL Combi-xt System. It performs enrichment of volatile or semi-volatile compounds during headspace analysis. A microtrap filled with adsorbent material, such as Tenax or activated charcoal is placed between the heated PAL Combi-xt Headspace syringe and the syringe needle. Using the HS syringe as a pump, a part of the gaseous phase of the pre-conditioned sample vial is pumped repeatedly through the microtrap. This system setup allows rapid, simple and efficient extraction of volatile and semi-volatile sample compounds. To gain sensitivity simply the number of pumping strokes can be increased or several different vials containing the same sample can be extracted. During thermal desorption into the GC Injector the microtrap is rapidly flash heated and the analytes reach the GC column as a narrow band. No cryofocussing is needed to obtain sharp peaks. To prepare the next extraction, the hot trap is re-conditioned outside the injector with clean purge gas.Specifications ITEX-2 OptionPumping Syringe Size: 1.3ml HD syringe with removable trapITEX-2 Trap: Stainless steel material, deacitvated by Siltek®:Needle: Injection Needle gauge 23, Point style 5 (side hole)Standard Trap Material: 44mg Tenax TA 80/100 meshExtraction Speed: selectable from 10µL/s up to 1000µL/sExtraction Strokes: Selectable from 1 - 999Extraction Volume: Selectable from 130µL - 1300µL/strokeDesorption Temperature:+5°C above ambient - 350°C selectable in 1°C incrementsHeating-up rate: up to 12°C/s Desorption Speed: 1µL/sec. - 500µL/sec. Pumping Syringe and Trap Cleaning: Inert gas purging, 30sec. - 3600min. Heated Pumping Syringe:+5°C above ambient - 150°C selectable in 1°C incrementsIncubator Oven:6 heated vial positions for 2mL / 10mL / 20ml vials+5°C above ambient - 200°C selectable in 1°C incrementsAgitation:Interval shaking 250rpm - 750rpm, selectable in 1rpm incrementsIncubation Time: Up to 999 minutes selectable in 1 second increments ITEX-2 upgrade kit including 2pc. ITEX traps containing proven industry standard adsorbentsITEX adsorption step out of a sample vialITEX-2 trap material examplesTenax TAVolatile and semivolatile compounds, temperature limit of 350°C Carbotrap/CarbopackNon-porous graphitized carbon blacks (GCBs)Hydrophobic properties minimized sample displacement by waterCarbosieve/CarboxenFor very volatile compounds, e.g. Vinylchloride, Freon compoundsChoose between two options to control your PAL Combi-xt ITEX-2 Option. For individual application requirements the standalone PC based Windows XP / Vista software Cycle Composer is available. For single keyboard operation of a whole GC/GC-MS system, the following third party PAL Combi-xt drivers are available*.Vendor SoftwareAgilent ChemStationAgilent EZChrom EliteDataApex ClarityDionex ChromeleonJustice Software ChromperfectLeco ChromaTOFShimadzu GCMSsolutionThermo Scientific XcaliburVarian StarVarian GalaxieWaters MasslynxWaters Empower * certain drivers may not support the ITEX cycleFlexible Software ControlITEX-2 parameter control by Cycle ComposerCycle Composer sample listSample conditioning Adsorption Desorption Trap cleaningSample is heated and / or agitated in a sealed sample vial until equilibrium is achievedThe loaded ITEX Trap is flash heated up to 350°C and desorbed into the hot GC Injector After thermal desorption the hot ITEX Trap is cleaned with inert flush gasITEX-2 Sample Extraction ProcedureThe ITEX Trap needle pierces the sealed sample vial and the heated gas tight syringe pumps the gas phase through the ITEX Trap Figure 2: Extracted Ion chromatograms for m/z 86 obtained by GC/MS in SIM mode.The Diacetyl Peak can be detected with at least 6 times better S/N value using ITEX rather than Static Headspace. The concentration of diacetyl in this beer sample was in the order of less than 10ppbFigure 1: TIC (m/z 29-400) of Volatile Organic Compounds in Beer. Additional components could be identified due to 10 x higher sensitivity of ITEX compared to Static Headspace. 1 1,1-Dichloroethylene2 Methylene chloride (dichloromethane)3 trans 1,2-Dichloroethylene4 1,1-Dichloroethane5 2,2-Dichloropropane6 cis-1,2-Dichloroethylene7 Chloroform8 Bromochloromethane9 1,1,1-Trichloroethane10 1,1-Dichloropropene11 Carbon tetrachloride12 1,2-Dichloroethane13 Benzene14 Trichloroethylene15 1,2-Dichloropropane16 Bromodichloromethane17 Dibromomethane18 cis-1,3-Dichloropropylene19 Toluene20 trans-1,3-Dichloropropylene21 1,1,2-Trichloroethane22 1,3-Dichloropropane23 Tetrachloroethylene24 Dibromochloromethane25 1,2-Dibromoethane (EDB)26 Chlorobenzene27 1,1,1,2-Tetrachloroethane28 Ethylbenzene29 m-Xylene30 p-Xylene31 o-Xylene32 Styrene33 Isopropylbenzene34 Bromoform35 1,1,2,2-Tetrachloroethane36 1,2,3-Trichloropropane37 n-Propylbenzene38 Bromobenzene39 1,3,5-Trimethylbenzene40 2-Chlorotoluene41 4-Chlorotoluene42 tert-Butylbenzene 43 1,2,4-Trimethylbenzene44 sec-Butylbenzene45 4-Isopropyloluene (p-Cymene)46 1,3-Dichlorobenzene47 1,4-Dichlorobenzene48 n-Butylbenzene49 1,2-Dichlorobenzene50 1,2-Dibromo-3-chloropropane51 1,2,3-Trichlorobenzene52 Hexachloro-1,3-butadiene (Hexachlorobutadiene)53 Naphthalene54 1,2,3-TrichlorobenzeneComparison of ITEX analysis versus Static Headspace Sample: Purge and Trap calibration mix (Restek Cat.No. 30431 502.2 CAL2000 Mega-Mix)EPA 502.2 (Calibration Mix) with ITEX Volatiles with ITEXBeer Ketones with ITEXStatic Headspace Parameter60°C / 10min / 1mL sample volumeITEX ParameterExtraction Speed:100µL/sec.Total Pumping Strokes: 50Temperature Pumping Syringe / Sample Incubation: 60°C / 10min.Desorption at 200°C, 15sec. splitlessChromatography: Injection: Splitless 15sec. at 250°C / Carrier gas: 0.2bar hydrogen Column: Rtx-502.2 60m x 0.32mm ID, 1.8µm filmTemperature Program: 40°C - 1min. - 10°C / min to 220°CDetection: FID 250°C Static Headspace Parameter80°C / 15min / 1ml sample volumeITEX ParameterExtraction Speed: 50µL/sec.Total Pumping Strokes: 10 x 1mLTemperature Pumping Syringe / Sample Incubation: 80°C / 15min.Desorption at 250°CTrap Material: Tenax TA 80/100 meshChromatography: Injection: Split 1:25 at 250°CCarrier gas: 200 kPa He at constant pressureColumn: DB-VRX 20m x 0.18mm i.d. / 1µm filmTemperature Program: 40°C - 5min. - 10°C / min to 250°C - 10min.MSD transfer line: 250°C (17 cm x 110µm i.d. restrictror, 28kPa)Detection: MS in Scan/SIM ModeScan: 29-400 amuSIM Ions monitored: 43, 57, 86, 100 (50ms dwell time)1234 5 67 89101112 13141516 1718192021 222324 25262728 293031 32333435363738394041424344454647484950515253 54ITEXHeadspace1 1-Propanol2 Ethylacetatate3 2-methyl-1-propanol4 Ethyl propanoate5 3-methyl-1-butanol6 2-methyl-1-butanol7 2-methyl propyyl acetate8 Ethyl butyrate9 3-methyl butyl acetate10 2-methyl butyl acetateDiacetyl12345 6789102.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00Static HeadspaceITEX11.80 12.00 12.20 12.40 12.60 12.80 13.00 13.20 13.40 13.60 13.80Static HeadspaceITEXDiacetylSYSTEMSample Capacity*up to 600 1ml micro vials (78 1ml vials standard) 294 2ml vials (98 2ml vials standard) 96 10ml or 20ml vials 4 deepwell microplates (96/384 wells) 8 standard microplates (96/384 wells) ( * depends on GC model)GC Mounting Kits Agilent Technologies 5890 / 6850 / 6890 | 7890Thermo Scientific GC 8000Top / TRACE GC / Focus GCVarian GC 3400 / 3600 / 3800 / 3900 / 430 / 450Shimadzu GC 14 / 17A / 2010 / 2014Perkin Elmer Autosystem XL / Clarus 400 / Clarus 500 / Clarus 600GL Sciences GC 353 / 393 / 4000Order details for ITEX Option (part no. PAL ITEX-2Option)Description 1pc ITEX-2 Syringe 1.3mL with M7 x 0.5 Fitting 1pc Replacement plunger 1.3ml 2pc ITEX-2 trap TENAX TA 80/100 mesh 1pc Trap heater incl. electrical connections 1pc Endplate left side 1pc Syringe heater side bracket 1pc CD-ROM including ITEX Cycle (requires Cycle Composer)ConsumablesITEX-2TrapTXTA 1pc ITEXTrap Tenax TAITEX-2TrapTXTA3 Set of 3pcs. ITEXTrap Tenax TA SYRC ITEX-2.-1.3 1pc replacement ITEX Syringe 1.3mLPLG ITEX-2.-1.3 Replacement plunger for 1.3mL syringe Specifications are subject to change without noticeCustom filled traps available on request Please inquire with your local distribution partnerPAL Combi-xt General Specifications System Type XYZ robot with syringe only concept, no tubing in sample pathLocal User Interface Control panel with 4 function keys, graphical LCD display,unique scroll knob for teach functionsRemote ControlCycle Composer control software Windows 2000 / XPThird party instrument drivers for all major GC/GC-MS SystemsMaintenanceAccessibility to all maintenance parts from frontPreventative maintenance kits availableElectrical Control1x RS232 / 1 x LAN (with optional PAL Upgrade Electronics)3x TTL Input2x Opto Coupler Input2x Relay OutputPower Requirements100-240V, 120W, 50/60HzEnvironment 4°C - 40°C constant temperature, < 80% humidity (non condensing)Weight ~10kg (without accessories)DimensionLength 828mm Depth 385mm Height 575mmElectrical Safety StandardsCAN/CSA C22.2 No. 61010-1 / ANSI/UL 61010-1 / EN 61010-1To learn more about the unique PAL-xt Series of GC/GC-MS sample injection systems or any of our LC/LC-MS sample handling systems contact your PAL System distributor.Static Headspace - Liquid Injection - SPME - ITEX Extraction combined in one single instrumentGet P&T sensitivity without the cost of a P&T systemRapid & efficient sample enrichment of volatile & semi-volatile compounds in solid, liquid and gaseous samplesIn-tube extraction and direct thermal desorption usingproven industry standard adsorbentsSyringe only concept for transparent sample handling, no sample loops, no transfer lines, no switching valvesNo GC injector modifications, no cryo-focussing necessaryTop mounted on GC's, saves valuable bench spaceInterfaces with any PAL Combi-xt System controlled by all major GC/GC-MS Systemswww.palsystem.comSYSTEMDistributed by: