Mimetic Blue AP HL P6HF - 3115 - User Guide

Alkaline phosphatase (orthophosphoric monoester phosphohydrolase [E.C. 3.1.3.1]) is a widely used reagent in biochemistry, molecular biology and immunodiagnostics. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins (e.g. casein) as well as nucleic acids. Alkaline phosphatase has also been shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A[1]. Mimetic Blue® AP HL P6HF is an affinity chromatography adsorbent that has a specifically designed Mimetic Ligand™ with a phosphonate group for the purification of alkaline phosphatases.