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C A T A L O G O F M I C R O P L A T E S & M I C R O P L A T E E Q U I P M E N TN O R T H A M E R I C A N E D I T I O NWelcome to the first North American edition of the PorvairSciences Microplate Catalog. Many scientists around the world have come to rely upon ourMicroplate Catalog as a primary source of information that enablesthem to choose the right assay, storage or SPE/Crash plate. This latest edition brings together information on Porvair Sciencescomprehensive microplate and microplate instruments productrange. Reflecting our commitment to continuous microplatetechnology innovation - the 2013 catalog introduces a suite ofexciting new products.For example, there’s our new GLASS LINED PLATE – 96 highquality glass vials in a sealable 48mm storage block - ideal forUHPLC applications. Our instrument range boasts two newthermal plate sealers. MicroSeal is a budget priced heat sealer withgreat performance, while our popular Miniseal Plus is quicker,quieter, safer and more economical than before. Add to this newglass-bottom imaging plates, more sterile plate options and afantastic new range of heat seals and you will see that this catalogis an indispensable reference guide to any laboratory using orinterested in using microplates.We are committed to producing top-quality microplates that givereproducible results every time, using only carefully selectedplastics and polymers that will not affect your assays. Our platesmay cost a little more than the commodities on offer from low-costproducers in emerging nations, but our attention to materialsselection, superior molding techniques and thorough qualitycontrol procedures will pay dividends when you run your assay orretrieve your samples from long-term storage. If sensitivity,accuracy and reproducibility are important to your long-termstorage or assays, you cannot afford to buy cheap, poor qualitymicroplates. Our website contains technical notes on the type ofcontamination you can expect to find with these inferior plates.Porvair Sciences is one of the largest global manufacturers of ultra-clean microplates for life science, synthetic chemistry and manyother applications. Our modern Class 10000 clean room facility inthe United Kingdom is responsible for production of all of ourclear-bottom plate products, including the highly-acclaimed glassbottom Krystal plates. The same highly experienced UK team alsoprovides first-class customer service to our customers anddistributors worldwide. Should you be interested in evaluating andtesting any of our microplates - just give our friendly team a call, orsend them an email asking for a free sample pack of yourmicroplate of choice. You can find details of your local distributorby visiting our website at www.microplates.com. We cover theUSA and Canada with a full spread of local, regional and nationaldistributors, so that there is bound to be one near your lab.With a proud history of innovative microplate manufacturingdating back to 1992, Porvair Sciences' mission is to become yourpreferred global partner for microplate products. Our technical andsales teams are at your disposal – so take a look through thiscatalog and you will see a wide array of microplate products andinstruments designed to enable you to get better results faster andmore consistently. CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION2 W W W . M I C R O P L A T E S . C O MF O R E W O R DPorvair Sciences products are designed for use in research environments andare not suitable for clinical, diagnostic or medical use.All trademarks are acknowledged.Varian® is a registered trade mark of Agilent Inc.Polymerase Chain Reaction (PCR) is a process covered by patents owned byHoffmann-La Roche.SealPlate™, ThermalSeal RT2, RT5, RT2RR™, Texan™, AbsorpMax™,AeraSeal™, AlumaSeal™,EZ-Pierce™ and X-Pierce™ are trademarks of Excel Scientific Inc.MicroLute™, MaxiLute™, Ultravap™, MiniSeal™, MiniVap™,MicroStream™, Krystal™, Chromatrap™ and BioVyon™ are all trademarksof Porvair Plc.Megabace™ is a registered trademark of Life Technologies Inc.Specifications subject to change without notice. E&OE.Pictures taken inside the Porvair Sciences clean room inWrexham, UK, showing production of clear bottom assayplates.3C O N T E N T SW W W . M I C R O P L A T E S . C O MContentsStorage/Collection96-well deep square Page 596-well deep round Page 696-well shallow round Page 796-well round low profile Page 896-well glass vial storage plate Page 8384-well Page 9Large volume Page 10Life SciencesChoosing the correct assay plate Page 12Solid bottom assay plates Page 14Clear bottom assay plates Page 18Glass bottom assay plates Page 22 UV clear bottom assay plates Page 24Bacti-growth plates Page 25Components for assay development Page 26Solid phase extractionMicroLute Page 27Combinatorial MicroLute Page 28MaxiLute Page 29P3 Protein Crash Plates Page 30SPE starter pack Page 31EquipmentVacuum manifolds Page 32MiniVap microplate evaporator Page 34Ultravap RC microplate evaporator Page 36Mat Capper and De-capper Page 38MicroSeal manual thermal plate sealer Page 39MiniSeal II semi-automatic sealer Page 40Seals and foils Page 41Custom manufacture Page 45Chemical resistance table Page 46CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTIONCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTIONS T O R A G E / C O L L E C T I O N – 9 6 - W E L L D E E P S Q U A R EW W W . M I C R O P L A T E S . C O M4Are your plates whiter than white?Or do they harbour a dirty secret?Deep well polypropylene microplates are commonly used forsample storage in life science laboratories. An essential aspectof the manufacture of these plates is the selection of cleanraw materials for injection molding. Polypropylene is an inertand heat resistant material, ideal for sample storage.However, all grades of polypropylene are not the same, forexample, injection molding grades of polypropylene oftenhave high concentrations of chemicals to aid the moldingprocess.This allows rapid production of plates and lower costs, butmay compromise the integrity of samples or compoundswhich are stored in such plates for extended periods. Theproblem is particularly acute where compounds are stored assolutions in solvents, such as methanol or DMSO, as theseexcellent solvents have a tendency to extract from thepolypropylene base material any added extractablecompounds such as mold release agents or polymer flowimprovers.Porvair Sciences has carried out tests on many manufacturers’deep well plates to establish levels of extractables in each. Atypical example is illustrated. Extractables testing wasperformed by adding a volume of methanol, sufficient to halffill the wells to each test plate. An internal standardcomprising methanol spiked with 10 mg/ml of caffeine wasused to give a consistent peak. Samples were taken from eachwell and subjected to analysis on a GC-MS system withsplitless injection @ 250°C, using an HP - 5MS capillarycolumn. A temperature gradient was programmed such that:Ramp 30°C/minInitial temp 70°C Hold 2 minFinal temp 310°C Hold 5 minResults from the GC-MS showed good performance from thePorvair microplate, with a clean caffeine peak and nosignificant levels of leachable or extractable compounds. PlateB shown here, is a typical example of the other commercialmicroplates tested, which included two major Germanmanufacturers and several other well-known suppliers. Thesevariously displayed extra peaks with a variety of retentiontimes across the run, indicating several differentcontaminants. Further analysis of these extra peaks revealedthat they were, indeed, mostly recognized chemicals used asadditives in the polypropylene to assist the injection moldingprocess.From this it can be seen that it is essential to select the correctgrade of polypropylene material for storage platemanufacture. The full report can be downloaded from ourwebsite.Plate B8000007000006000005000004000003000002000001000000AbundanceTime5.09 5.45.716.026.346.656.967.277.59 7.98.218.528.849.159.469.7710.110.410.7 1111.311.6 1212.312.612.913.213.513.814.114.5PorvairAbundance8000007000006000005000004000003000002000001000000Time5.09 5.45.716.026.346.656.967.277.59 7.98.218.528.849.159.469.7710.110.410.7 1111.311.6 1212.312.612.913.213.513.814.114.514.896-well deep square44mm heightThe family of 96 square well plates wasdesigned so that it would make the interchangeof plates simpler in automated systems. Allthree plates have the same geometry and theonly variable is the well depth.? Made from virgin polypropylene? Tested for low extractables? Tight specification levels? V bottom to allow total liquid removal, partial collection and to aid re-suspension? No inner edges to allow better collection of magnetic beads? Manufactured under DNase/ RNase free environment? Working volumes of 350µl, 1ml, 1.6ml and 2ml? Raised well rims to improve heat sealing? Conical base which aids sample concentration, reconstitution and centrifugation? Sterile and non-sterile versions availableCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION5S T O R A G E / C O L L E C T I O N – 9 6 - W E L L D E E P R O U N DW W W . M I C R O P L A T E S . C O MStorage plates 96 square wellDescription Well volume Sterile Use cap mat Quantity/case Cat. no.Polypropylene, pyramid bottom 2.0ml – 219004 50 219009Polypropylene, pyramid bottom, sterile, inner bag of 5 2.0ml ? 219019 50 219027Polypropylene, round bottom 1.6ml – 219004 50 500066Polypropylene, pyramid bottom 1.0ml – 219004 50 219008Polypropylene, pyramid bottom, sterile, inner bag of 5 1.0ml ? 219019 50 219026Polypropylene, pyramid bottom 350µl – 219004 50 219006Polypropylene, pyramid bottom, sterile, inner bag of 5 350µl ? 219019 50 2190251.6mlDrawing of 500066 available on request.W W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION6S T O R A G E / C O L L E C T I O N – 9 6 - W E L L S H A L L O W R O U N D96-well deep round42mm heightThe plates are made in virgin polypropyleneto minimize extractables. Working volume is1ml per well and total volume is 1.1ml.Colored plates aid identification whenretrieving from storage. Black plates aresuitable for storing light sensitive compounds.? Manufactured from pre-tested polypropylene for low extractables? Alphanumeric grid-referencing? DNase/ RNase free? Packed in sealed sleeves of 5 plates? Rimmed version to stop cross contamination and enable a better seal? Non rimmed version to allow insertion of vials, or where the seal is not crucial? Cylindrical well with round bottom for optimal mixing and recovery? Very easy to use with automated sample handling systems? Can be stored at -80ºCPolypropylene storage plates roundRim and bottom well shape Colour Well volume Sterile Use cap mat Quantity/case Cat. no.Raised- round Natural 1ml – 219036 50 219002Raised- round Natural 1ml ? 219042 50 219012No rim- round Natural 1ml – 219036 50 219037Raised- round Blue 1ml – 219036 50 219432Raised- round Red 1ml – 219036 50 219422Raised- round Black 1ml – 219036 50 219412NO RIMWITH RIMCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION7S T O R A G E / C O L L E C T I O N – 9 6 - W E L L R O U N D L O W P R O F I L EW W W . M I C R O P L A T E S . C O M96-well shallow round14.7mm heightPorvair Sciences has developed a number ofstorage/collection plates to help in thefields of cell biology, molecular biology, drugdiscovery, combinatorial chemistry, screeningand genomics. The plates are manufacturedunder clean room conditions and a significantnumber are DNase/RNase free. They aremainly made from polypropylene, an inertmaterial giving heat and solvent resistantqualities. Porvair Sciences offers the largestcombination of well shape, number of wellsand well volume on the market. Each is madeto the ANSI/SLAS format for compatibilitywith most readers/washers and automatedequipment.Porvair Sciences offers three plates with wellcapacities of 350µl, 270µl and 220µl. Theyhave flat-, round- or V-bottoms and can beused for compound storage and culturing.? Manufactured in polypropylene? Alphanumeric grid reference? Round and V-bottom allow greater liquid removal and particulate collection? Raised rims improve sealing and stop cross contaminationStorage plates roundRim and bottom well shape Material Well volume Sterile Quantity/case Cat. no.No rim, flat Polypropylene 350µl – 100 208003Raised-round Polypropylene 270µl – 100 209003Raised-V Polypropylene 220µl – 100 210003ALL DIMENSIONS IN MMS T O R A G E / C O L L E C T I O N – 3 8 4 - W E L LW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION8? Store aggressive solvents in glass vials? Chemically resistant? No trace impurities for UHPLC? Prevent evaporation with matching cap mat? Allows stacking in higher densities? Minimizes space needed for compound storage? Pure virgin polypropylene? Matching snug-fitting cap mat96-well glass vial storage plateDescription Qty/case Cat. no.96-well glass vial storage plate 12 229230Square well silicone cap mat for 229230 5 22909196-well round low profileDescription Working well vol Sterile Qty/case Cat. no.96-well round low profile plate 1ml – 50 21925096-well round low profileA new introduction to the Porvair range is a lowprofile 1.1ml 96-well round plate with a height ofjust 27mm. This allows more plates to be stored orstacked in a given space. The plates are made withextractable-free polypropylene for excellent results.A specific anti-evaporation cap mat is also availablefor this plate manufactured from thermoplasticelastomer.96-well glass vialstorage plate44mm heightThe new Porvair glass vial storage plate combines96 borosilicate glass vials of 700µl into a rigidpolypropylene carrier plate for ease of storage andtransportation. The glass vials are round bottomedand the plate can be capped with our matchingsquare-well cap mat to prevent evaporation orcontamination. This is the zero-leachates solutionfor UHPLC and where organic solvents must bestored in a plate footprint. Dimensioned drawing available on request.W W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION9S T O R A G E / C O L L E C T I O N – L A R G E V O L U M E384-wellPorvair Sciences has a selection of 384-wellpolypropylene plates to suit most applications.All of them are made from pre-testedpolypropylene in Class 100000 clean rooms.? Manufactured for high density sample collection / storage? Two sizes: 58µl and 300µl per well working volume? Each are designed to allow almost total removal of liquid sample? Extra flat – allows plate sealing? Storage temperature down to –80ºC? Available sterile and non sterile? All manufactured to ANSI/SLASspecificationsStorage plates 384-wellWell shape, top & bottom Working well vol Sterile Qty/case Cat. no.Square-round 58µl – 60 224001Square-V 120¨µl – 100 300003Square-V 300µl – 48 219040Square-V 300µl ? 48 219041S T O R A G E / C O L L E C T I O N – L A R G E V O L U M EW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION10High volume deep well microplatesWell shape, bottom shape No of wells Working well vol Sterile Lid Qty/ case Cat. no.Rectangle, V-bottom 24 10ml – – 25 360013Rectangle, V-bottom (bulk pack) 24 10ml ? – 25 360115Rectangle, V-bottom 24 10ml – ? 25 360077Rectangle, V-bottom (single pack) 24 10ml ? ? 25 360079Rectangle, V-bottom (with bar code) 24 10ml ? ? 25 360080Rectangle, round-bottom 24 10ml – – 25 360117Rectangle, V-bottom 48 5ml – 25 360002Rectangle, V-bottom 48 7ml – 30 360004Deep well microplate accessoriesDescription Quantity/pack size Cat. no.Centrifuge support plates for 219008 & 219026 2 228018Centrifuge support plates for 219009 & 219027 2 22801948-well features:? Two versions:? 5ml/well, 44mm high? 7ml/well, 68mm high24-well features:? 24 wells with a working capacity of 10ml/well? Standard height (44mm) of a deep well plate? Sterile or non sterile versions? Lidded version availableCentrifuge support plateThe support plate is designed to fit into theskirt of the deep well plates. This then allowsthe plate to be used in a centrifuge withoutthe risk of the plate distorting and causing thewell to leak.? Available for the 2ml and 1ml square deep well plates? Easily fitted below platesLarge volumeThe range of large volume plates is designed to meet specialrequirements. Focused on the combinatorial, environmentaland food technology markets, it allows large volumes ofsamples to be transported in recognised ANSI/SLAS formatmicroplates, to allow greater automation. Plates will acceptthe Porvair universal lid (see page 55). All plates aremanufactured from virgin polypropylene, can be heat sealedand stored for prolonged periods at -80ºC.360004360013 360002CENTRIFUGE SUPPORT PLATEW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESEQUIPMENTSOLID PHASE EXTRACTION11S T O R A G E / C O L L E C T I O N – L A R G E V O L U M ECUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION12W W W . M I C R O P L A T E S . C O ML I F E S C I E N C E S – C H O O S I N G T H E C O R R E C T A S S A Y P L A T EChoosing the correct assay plateChoosing the correct microplate for your application can mean thedifference between indifferent and great results.There are three basic methods of obtaining useful optical data frommicroplate-based samples. The simplest method is absorbancemeasurement. Where greater sensitivity is required, fluorescencemeasurements are preferred. Sensitivity can be ten times greaterthan simple absorbance measurements. The third methodinvolves luminescence, a naturally occurring phenomenonexhibited by certain animal and plant species which can emit light.This is a biological adaptation of a process which can also be seen aspurely chemically-driven reactions, in which case it is referred to aschemiluminescence.Microplate readers are designed to read from either the top or thebottom of a microplate. Top reading instruments rely onmeasuring reflected light above the wells. A good solid brightwhite plate is best for these absorbance measurements and asolid black plate for fluorescence readings. Bottom reading unitsilluminate the sample from above and then use detectors placedbelow the plate to measure the absorption orfluorescence/luminescence emission. This necessitates the use ofclear-bottomed plates. The requirement is to transmit the lightwavelengths of interest.Visible wavelength range (900-350nm) measurements requireonly clear plastic bases, while readings between 220nm and350nm will require a UV-transparent material. This can beQuartz sheet or a modern polymer such as Cyclo-Olefin Co-Polymer (COP/COC). Optical glass sheet is used where visible rangedetection is combined with confocal optics or whole plate imagingwhich requires a very clear uniformly-flat base.Simple 96-well microplates for ELISA type assays are made fromsolid clear polystyrene with no additives. These are adequate forclinical and diagnostic tests, ELISA assays and any colorendpoint determination with relatively high absorbance.Typically they are available with flat well bottoms, giving high surfacearea, round well bottoms for good mixing or V-wells for high liquidrecovery.Crosstalk can also be an issue in bottom-reading absorbance andfluorescence measurements. To address this applicationschallenge, Porvair Sciences has developed the Krystal 2000zero-crosstalk plates in which individual clear wells aremoulded into either a white or black matrix. The black or whitebase material also projects down below the clear well bottom tofurther reduce the possibility of crosstalk.By carefully selecting the correct plate type for the assay, it is possibleto significantly improve results. By following the simple guidelinesset out here, those tasked with assay development can ensurethat their final assay has the best possible chance of success.The table summarizes the choices available for Assay Plateselection and indicates the plate type most likely to give the bestresults. Porvair Sciences will be happy to supply samples of theirplates for evaluation free of charge, as different detection systemsand differing assay development may lead to variances in resultsbetween ostensibly similar plate types.L I F E S C I E N C E S – C H O O S I N G T H E C O R R E C T A S S A Y P L A T EW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION13An introduction to clear bottomed assay microplatesPorvair Krystal series clear bottom plates are now recognizedas industry-leading tools for Pharma drug discovery andscreening. Our standard clear polystyrene bottom plateshave bases just 0.4mm thick and are moulded from highlypolished tools to reduce flow lines, artefacts and scratches,guaranteeing good results with all types of bottom-readinginstruments. For the more modern demanding confocalreaders, Porvair glass bottom plates have been shown byleading research institutes to be unsurpassed for flatness,linearity and optical clarity. Our sparkling pure borosilicateglass is just 175µm thick with a flatness tolerance across theplate of +/- 15µm ensuring superb crisp images every time.We supply our Krystal clear plates treated for cell culturewith a low pressure gas plasma that produces an evensurface treatment right across the plate. It is particularlysuited to cell lines which do not grow easily on plastics. Ourplastic-bottomed plates are ultrasonically welded together –a tried and tested method which both guarantees a perfectseal every time and eliminates the inconsistencies seen withlaser membrane welding used by other manufacturers. Onlybiocompatible adhesives are used for glass bottom plates,cured by UV radiation to ensure that the finished plates aresolvent-free, have very low autofluorescence and will allowgood cell growth.Choosing the correct assay plateAbsorbance assays –Top reading instrumentsHigh signal from majority of wells e.g. test kits, ELISA Use a simple solid clear plateLow signal from some or all wells e.g. kinetics or genetic marker assays Use a solid white plate to boost the signalFluorescence assays –Top Reading InstrumentsUse a solid black plateLuminescence assays –Top reading instrumentsBio- and Chemi- Luminescence use the same platesGood signalUse solid white platesPoor signal or high dynamic range across the plateUse solid black with white wellsBottom reading instrumentsNormal visualisation: white plates with clear plastic bottomsConfocal visualisation: white plates with glass bottomsFor very sensitive assays – zero crosstalk white plate with individual clear wellsBottom reading instrumentsVisible wavelength range 350-700nmHigh signal from majority of wells e.g. GFP, ReporterGene kits, Use a black clear-bottomed plateLow signal from some or all wells e.g. poor sensitivity assays, low specificity Use a black zero crosstalk clear bottom plate to boostthe signal-to-noise ratioUV wavelength range 220-350nmUse black clear-bottomed COP platesConfocal or whole plate imaging:Use black glass-bottomed platesBottom reading instrumentsUse a white clear-bottomed plateCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION14L I F E S C I E N C E S – S O L I D B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O M96-well clear platesFor routine adsorption, absorption, ELISA,mixing and storage applications the standardrange of 96-well assay plates offers theperfect mix of affordability and high quality.? Manufactured from high quality crystal polystyrene? Flat bottom for spectrophotometric work? V-bottom minimizing residual liquid? Round (U) bottom forcell/particulate collection? Robot compatible? Working volumes from 275µl down to 10µlSolid assay platesPlate description Sterile Colour Qty/pack Cat. no.96 wells of 350µl with a flat bottom – Clear 100 20800496 wells of 270µl with a round bottom – Clear 100 20900496 wells of 220µl with a V bottom – Clear 100 21000496 wells of 350uL with a flat bottom, medium bind polystyrene for ELISA tests ? Clear 200 50004596 wells of 350uL with a flat bottom, standard bind polystyrene for ELISA tests ? Clear 200 500046L I F E S C I E N C E S – S O L I D B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION1596-well black, white &black with white wellsPorvair Sciences range of solid bottom 96-well assay plates is specifically designed forabsorbance, fluorescence, luminescence andscintillation applications. The design uses themost popular 96-well format with standard‘chimney’ wells to overcome optical crosstalkand contamination.These plates are designed to give optimumresults from any instrument which reads fromthe top of the plate.The acclaimed composite black/white platehas been shown to be ten times moresensitive than a standard white plate forchemi-luminesence assays.? Black plates designed for top reading fluorescence instruments? Black plates have low background fluorescence and minimize light scattering? White plates maximize signal for luminescence readers? Highly polished well to give better readings? Specially designed plate featuring a white well set in a black matrix to overcome the problem of a very high luminescence causing false positives? Working volumes of 350µl/well? Complies with the standard ANSI/SLAS format? Alphanumerically labeled wells mean samples can be easily tracedSolid polystyrene assay platesPlate description Colour Treatment Sterile Lid Qty/pack Cat. no.96 wells White – – – 50 20450396 wells White – – – 100 20400396 wells White Hi protein binding – – 100 20400696 wells White Tissue culture ? ? 50 20451296 wells White Tissue culture ? ? 100 20401296 wells Black – – – 50 20550396 wells Black – – – 100 20500396 wells Black Hi protein binding – – 100 20500696 wells Black Tissue culture ? ? 50 20551296 wells Black Tissue culture ? ? 100 20501296 wells Black with white wells – – – 100 301004CUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION16L I F E S C I E N C E S – S O L I D B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O M384-well plates384-well assay plates from Porvair Sciencesare available in black, white and clear. Thesehave been optimized for fluorescence,luminescence/scintillation and ELISA/turbiditymeasurements respectively. There are twoworking volumes: 30µl/well or 120µl/well.All 384-well assay plates are available instandard, high bind and tissue culture treatedformats.Round well low volumeThe low volume 384-well assay plate isdesigned for high throughput screening with30µl total volume to reduce the amount ofsample and reagent required for the assay.Square well platesThe 384-well format, with its higher density of wells, allowsmore work to be carried out in a standard sized plate, aidinghigher throughput. Well volume is 120µl per well. All of theseplates are manufactured from high quality polystyrene.? Designed to reduce well-to-well crosstalk? Black plates have low background fluorescence and minimize light scattering? White plates enhance bio- & chemi-luminescence signals and have low background luminescence and fluorescence? Well working volumes of 100µl? Rounded square at the well bottom to reduce wicking? Low residual volume? Designed to the standard ANSI/SLAS format? Alphanumerically labeled wells mean samples can be stored and easily traced? Specially rounded well to allow pipette tip access to the bottom of the well without interference? Rounded edges to reduce droplet formation? Provides a total liquid capacity of 30µl or 5µl per well? Unique moulding technique to achieve superb flatness to a tolerance of +/-0.2mm? Standard height plate (14.7mm) for greater ease of automation? Standard ANSI/SLAS formatNEWVery low volume 5µl clearTC plate 500079L I F E S C I E N C E S – S O L I D B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION1730µl/wellSolid assay platesPlate description Colour Treatment Sterile Lid Qty/pack Cat. no.384 wells of 120µl Clear – – – 50 221503384 wells of 120µl Clear – – – 100 221003384 wells of 120µl Clear Hi protein bind – – 100 221012384 wells of 120µl Clear High bind with lid – ? 50 221006384 wells of 120µl Clear Tissue culture ? ? 50 221509384 wells of 120µl Clear Tissue culture ? ? 100 221009384 wells of 120µl White – – – 50 222503384 wells of 120µl White – – – 100 222003384 wells of 120µl White Hi protein bind – – 100 222012384 wells of 120µl White Hi protein bind with lid – ? 50 222006384 wells of 120µl White Tissue culture ? ? 50 222509384 wells of 120µl White Tissue culture ? ? 100 222009384 wells of 120µl Black – – – 50 223503384 wells of 120µl Black – – – 100 223003384 wells of 120µl Black Hi protein bind – – 100 223012384 wells of 120µl Black Hi protein bind with lid – ? 50 223006384 wells of 120µl Black Tissue culture ? ? 50 223509384 wells of 120µl Black Tissue culture ? ? 100 223009120µl/wellSQUARE WELL384 wells of 30µl Clear – – – 50 221103384 wells of 30µl Clear Tissue culture ? ? 50 221109384 wells of 30µl White – – – 50 222103384 wells of 30µl White Tissue culture ? ? 50 222109384 wells of 30µl Black – – – 50 223103384 wells of 30µl Black Tissue culture ? ? 50 223109384 wells of 5µl Clear Tissue culture ? ? 180 500079ROUND WELLCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION18W W W . M I C R O P L A T E S . C O ML I F E S C I E N C E S – C L E A R B O T T O M A S S A Y P L A T E S24-well Krystal™ black &whiteClear bottom plates allow the cells grown onthe bottom surface to be viewed using aninverse microscope. In addition, light-emittingassays can be measured from the bottom.Porvair Sciences collection of clear bottom 24-well Krystal plates are optimized forfluorescence and luminescence. The specialdesign of the plate virtually eliminates well-to-well light crosstalk inherent with otherclear-bottomed microplate designs, enablingunmatched accuracy, higher sensitivity andbetter repeatability of photometric readings.For photometric applications where samplesare typically large, Porvair Sciences range ofunique black or white Krystal 24 platesprovides a perfect solution. Offering 24 highvolume (3.1ml) wells in the same convenientfootprint of a standard 96-well plate, theKrystal 24 provides a large surface area perwell, enabling efficient cell growth. All tissueculture treated plates are supplied lidded andsterile, in individual bags.? Opaque walls to prevent well-to-well crosstalk? Clear plate bottom permits direct microscopic viewing? Base plate thickness 0.75mm? For use with top or bottom reading instruments? Conforms to the standard ANSI/SLAS format? Well volume of 3.1ml? Constructed with ultra-pure polystyrene components polished to give exceptional photometric performance and optimized for cell growth Krystal™ 24-wellColour Treatment Sterile Lid Qty/pack Cat. no.White – – – 68 303002White Hi protein binding – – 68 303004White Tissue culture ? ? 56 303006Black – – – 68 303008Black Hi protein binding – – 68 303010Black Tissue culture ? ? 56 303012W W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION19L I F E S C I E N C E S – C L E A R B O T T O M A S S A Y P L A T E S96-well Krystal™ blackand whitePorvair Sciences clear bottom 96-well Krystalplates are optimized for fluorescence andluminescence. Proprietry ultra-sonic welding isused to assemble the clear bases to the solidtop plate in our clean room. This designprovides flat, optically clear bases to each wellwith no leakage, enabling excellent accuracyand reproducibility of photometric readings.All tissue-culture treated plates are suppliedlidded and sterile, in individual bags.? Opaque walls to prevent well-to-well crosstalk? Clear plate bottom permits direct microscopic viewing? Thickness of bottom 0.75mm? For use with top or bottom reading instruments? Standard ANSI/SLAS format? Well volume of 350µl? Constructed from ultra pure grade polystyreneKrystal™ 96-wellColour Treatment Sterile Lid Qty/pack Cat. no.White – – – 50 214503White – – – 100 214003White Tissue culture ? ? 50 214506White Tissue culture ? ? 100 214006White Tissue culture ? ? 100 (4 x pack of 25) 214030Black – – – 50 215503Black – – – 100 215003Black Tissue culture ? ? 50 215506Black Tissue culture ? ? 100 215006Black Tissue culture ? ? 100 (4 x pack of 25) 215030CUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION20L I F E S C I E N C E S – C L E A R B O T T O M A S S A Y P L A T E SW W W . P O R V A I R - S C I E N C E S . C O M384-well Krystal™ blackand whiteThe Krystal 384-well microplate rangeincorporates novel square wells with a largeliquid capacity of 120µl per well, increasingthe achievable sensitivity of most HTS assays.Using a proprietary manufacturing technique,the Krystal 384 offers market leading plateflatness (+/- 0.1mm tolerance) that translatesinto a significant increase in measurementprecision and elimination of read errors whenperforming cell based assays using fluorescentor luminescent imaging. All tissue-culturetreated plates are supplied lidded and sterile,in individual bags.? Opaque walls to prevent well-to-well crosstalk? Clear plate bottom permits direct microscopic viewing? Thickness of bottom 0.40mm? For use with top or bottom reading instruments? Maintaining the standardANSI/SLAS format? Well volume of 120µl? Constructed from ultra pure grade polystyreneKrystal™ 384-wellColour Treatment Sterile Lid Qty/pack Cat. no.White – – – 100 311001White Tissue culture ? ? 50 311503White Tissue culture ? ? 100 311003White Tissue culture ? ? 100 (4 x pack of 25) 311030Black – – – 100 312001Black Tissue culture ? ? 50 312503Black Tissue culture ? ? 100 312003Black Tissue culture ? ? 100 (4 x pack of 25) 312030L I F E S C I E N C E S – C L E A R B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION2196-well Krystal™ 2000black and whiteThe unique Krystal 2000 plate range fromPorvair Sciences has been optimized forluminescence and fluorescence assays. Apatented manufacturing process, using a‘two-shot’ mold, provides clear, individualwells in an opaque matrix. The special designof the plate totally eliminates the well-to-welloptical crosstalk inherent with otherclearbottomed microplate designs, givingunmatched accuracy, sensitivity andrepeatability of photometric readings. Veryhigh signal-to-noise ratio and low detectionlimits can be achieved with this superb plate.All tissue-culture treated plates are suppliedlidded and sterile, in individual bags.? Raised rims to prevent well-to-well crosstalk? Lowered bottom rim to stop lateral light piping? Clear well bottom permits direct microscopic viewing? For use with top or bottom reading instruments? Designed to the standard ANSI/SLAS format? Total well volume of 350µl? White matrix provides for maximum reflectivity, allowing high sensitivity luminescence assays? Black matrix provides a quenching of background fluorescence, reducing false positives? Constructed from ultra pure grade polystyrene11.30 9.0011.4585.606.506.00ALL DIMENSIONS IN MM14.7074.30127.6014.30113.30Krystal™ 2000 96-wellColour Treatment Sterile Lid Qty/pack Cat. no.White – – – 50 301510White – – – 100 301010White Hi protein binding – – 100 301018White Tissue culture ? ? 50 301512White Tissue culture ? ? 100 301012White Tissue culture ? ? 100 (4 x pack of 25) 301070Black – – – 50 301502Black – – – 100 301002Black Hi protein binding – – 100 301014Black Tissue culture ? ? 50 301506Black Tissue culture ? ? 100 301006Black Tissue culture ? ? 100 (4 x pack of 25) 301060Clear cupColoured matrixCross-section of wellCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION22L I F E S C I E N C E S – G L A S S B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O MKrystal™ 24-well glassbottom platesKrystal™ glass bottomplatesManufactured for whole-plate CCD imagingand laser detection applications, Krystal glassbottom plates consist of a polystyrene upperpart and a clear borosilicate glass sheet fixedto the base with a proprietary adhesive. Thisprocess results in consistent flatness of thebase and gives improved light transmissionwhile maintaining a flat optical plane forgrowing cells. The nominal cut-offwavelength of 335nm allows mostfluorescence assays to be excited or readthrough the glass bottom. All plates aresupplied lidded.? 175µm glass thickness? +/- 15µm flatness across base? 335nm UV optical cut-off? Industry-standard well layout? Very low autofluorescence? High degree of planar flatness? Biocompatible adhesive? Resistant to alcohol, DMSO and PBS? Robot friendly? Manufactured to ANSI/SLAS specificationGlass bottom assay platesNumber of wells Description Sterile Lid Pack size Cat. no.24 wells Black with lid, single pack – ? 10 32404124 wells Black with lid, single pack ? ? 10 32404224 wells White with lid, single pack – ? 10 32405124 wells White with lid, single pack ? ? 10 324052L I F E S C I E N C E S – G L A S S B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION23Krystal™ 96-well glassbottom platesGlass bottom assay platesNumber of wells Description Sterile Lid Pack size Cat. no.96 wells Black with lid, single pack – ? 10 32400196 wells Black with lid, single pack ? ? 10 32400296 wells White with lid, single pack – ? 10 32401196 wells White with lid, single pack ? ? 10 324012Krystal™ 384-well glassbottom platesGlass bottom assay platesNumber of wells Description Sterile Lid Pack size Cat. no.384 wells Black with lid, single pack – ? 10 324021384 wells Black with lid, single pack ? ? 10 324022384 wells White with lid, single pack – ? 10 324031384 wells White with lid, single pack ? ? 10 324032CUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION24L I F E S C I E N C E S – U V C L E A R B O T T O M A S S A Y P L A T E SW W W . M I C R O P L A T E S . C O MKrystal UV clearbottomed microplatesRecently, scientists have begun using assaychemistries which require excitation ordetection wavelengths in the far UV region,below 350nm. To enable our customers todevelop assays in this area, Porvair Scienceswill be introducing a very high specificationrange of COP-bottomed, UV-transparentmicroplates during 2013. Please check withyour local Distributor for availability, samplesand pricing.? 220nm far UV cut-off? 96- and 384-well black versions? Industry-standard well layout? Very low autofluorescence? High degree of planar flatness? High chemical resistance to most solvents? Robot friendly? Cyclo Olefin Polymer construction with clear base? Precision engineered to meet ANSI/SLAS specificationKrystal UV COP-bottomed microplatesPackDescription size Cat. no.96-well KrystalCOP-bottomed plate 32 327002384-well KrystalCOP-bottomed plate 32 327001Detailed schematics available on requestOverall plate height = 13.06mmOverall plate height = 14.35mmL I F E S C I E N C E S – G R O W T H P L A T E SCUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION25W W W . M I C R O P L A T E S . C O MBacti-growth platesBacti-growth plates are specially packagedplates to allow the growth of bacteria, yeast,mammalian or insect cell lines.Using only the highest-grade raw materials,our plates are tested to ensure no molding orpolymer contaminants leach out into thesamples. This guarantees reproducibility andmaintains customer confidence. Each Bacti-growth plate consists of a deep well plate anda tight fitting lid, packed sterile in bags.? Plate well configuration available from 24- through to 384-well? Well volumes from 300µl through to 10ml per well? Round and square well available? Sealed in sterile bags of 5 plates? Robot friendly? Manufactured to the highest specifications? Conform to the ANSI/SLAS formatBacti-growth platesDescription Sterile Lid Qty/Pack Cat. no.24 x 10ml square wells, polypropylene with lid ? ? 25 21911596 x 1ml round wells, polypropylene with lid ? ? 25 21910196 x 2ml square wells, polypropylene with lid ? ? 25 219102384 x 300µl square wells, polypropylene with lid ? ? 20 219103219103219115For schematics of 219101 and 219102, please refer to 219009 (page 6) and 219002 (page 7).219101 219102 219103 219115CUSTOM MANUFACTURESTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION26L I F E S C I E N C E S – C O M P O N E N T S F O R A S S A Y D E V E L O P M E N TW W W . M I C R O P L A T E S . C O MComponents for assay developmentPorvair has many years of developing bespoke microplates and injection-molded components for Life Scienceresearch companies. As such, we are often asked for small numbers of the components used to make ourtwo-part plates. We are happy to make these available as standard items to aid your product or methoddevelopment programme.Description Qty/pack Cat. no.24-well A-Plate Krystal glass bottom plate, black 20 22921896-well A-Plate Krystal glass bottom plate black 20 22922024-well clear polystyrene lid for Krystal glass bottom plate 20 22921996-well clear polystyrene lid for Krystal glass bottom plate 20 229221Borosilicate glass sheet, 74 x 110mm x 175µm 100 22921796-well A plate Krystal polystyrene, black 126 50001196-well B plate Krystal polystyrene, clear 1350 50001296-well lid polystyrene, clear, with anti-condensation rings 300 500013Porvair Microplate Microarray / Chip Printing KitOur new Chip Printing Kit is a direct response to the many academic and research customers who haverequested our two-part clear bottom microplates as separate components. We are happy to provide either thetop (A plate) or bottom (B plate) components from our Krystal polystyrene clear bottom assay plates. We canalso supply the A plate and borosilicate glass sheet for our Krystal Glass Bottom microplates. This allows you touse either the B plate or the glass sheet as a substrate and to print your own microarray pattern directly on tothe smooth surface. The components can then be assembled with common solvents, such as methyl ethylketone (Krystal plastic plates) or cyanoacrylate glue (Krystal glass plates).Description Qty/pack Cat. no.96 well chip printing kit: contains 1 set of 20 A (upper) plates in blackpolystyrene and 1 matching set of 20 clear polystyrene B plates (bases).Suitable for solvent-assembly using methyl ethyl ketone.Chips can be printed with DNA chip on smooth side of the base. 20 500080Hand-held replicator tools for colony replicationThese easy to use disposable pin replicators are madefrom durable polypropylene and the 96-well tool comesin a choice of two useful lengths: 12mm and 25mmpins. They are DNA/RNA, DNAase/RNAase free.Manual Pin ReplicatorsDescription Qty/pack Cat. no.Disposable polypropylene pin tool 384 well plates, 12mm long, DNA/RNA, DNAase/RNAase free 120 500076Disposable polypropylene pin tool 96 well plates, 12mm long, DNA/RNA, DNAase/RNAase free 120 500077Disposable polypropylene pin tool 96 well plates, 24mm long, DNA/RNA, DNAase/RNAase free 120 500078S O L I D P H A S E E X T R A C T I O NCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENT27? Choice of popular sorbents? Packed bed volumes from 10mg to 100mg per well? Frit pore size 20µm? No channelling due to proprietary sorbent loading technique? Working volume of 2ml per well? Up to four times quicker than cartridge systems? Long drain directors locate accurately with collection plate to avoid cross contamination? Virgin polypropylene, tested and chosen for having no extractables? Plate designed to be automated, meets ANSI/SLAS specificationMicroLute™Solid phase extraction (SPE) is a method ofsample preparation that concentrates andpurifies analytes from solution by sorption,followed by elution of the analyte witha solvent appropriate for instrumentalanalysis, such as LC-MS. Porvair Sciencesoffers a complete range of products toimplement SPE.MicroLute™ is the original 96-well plateformat for SPE. This device consists of apolypropylene 96-well plate loaded with achoice of sorbent and sorbent volumes. Thefrits have a mean pore size of 20 microns andare made from microporous high-densitypolyethylene.W W W . M I C R O P L A T E S . C O MPorvair's flexible manufacturingapproach enables us to considermanufacture of small runs ofMicroLute™ devices packed withspecialty resins or sorbents of yourchoice. Typically, minimum runs of just50 plates can be producedeconomically. Please contact PorvairSciences for more details of this service.S O L I D P H A S E E X T R A C T I O NW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENT28? Accepted industry standard? Up to 2ml per well of sample? Low frit liquid retention? Virgin polypropylene? Polypropylene is tested and chosen for having no extractables? Manufactured to ANSI/SLAS standard to allow for automationCombinatorialMicroLute™Combinatorial MicroLute™ is based on thetried and tested MicroLute™ format. This is a96-well polypropylene plate with a bottompolyethylene frit already in place. It isdesigned to allow packing with any materialrequired for chemical synthesis. Two bottomfrit sizes are available, 10µm and 30µm,however, we recommend the use of 30µmtop frits to ensure a good flow rate in bothcases.Description 10mg 15mg 25mg 50mg 100mgC18 242001 244001 246001 248001 250001C8 242002 244002 246002 228002 250002C2 242003 244003 246003 248003 250003C1 242004 244004 246004 248004 250004PH 242005 244005 246005 248005 250005CH 242006 244006 246006 248006 250006CN-E 242007 244007 246007 248007 250007C18-OH 242008 244008 246008 248008 250008CN-N 242009 244009 246009 248009 25000920H 242010 244010 246010 248010 250010Si 242011 244011 246011 248011 250011CBA 242012 244012 246012 248012 250012PRS 242013 244013 246013 248013 250013SCX 242014 244014 246014 248014 250014NH2 242015 244015 246015 248015 250015PSA 242016 244016 246016 248016 250016DEA 242017 244017 246017 248017 250017SAX 242018 244018 246018 248018 250018PBA 242019 244019 246019 248019 250019ENV 242020 244020 246020 248020 –LMS 242021 244021 246021 248021 –PPL 242022 244022 246022 248022 –C18HF 242023 244023 246023 248023 –Certify 242024 244024 246024 248024 250024Certify HF 242025 244025 246025 248025 –Certify II 242026 244026 246026 248026 250026Certify II HF 242027 244027 246027 248027 –MicroLute™ packed with Varian sorbentCombinatorial MicroLute™Description Qty/pack Cat. no.Combinatorial Microlute™ plate, unpacked with bottom frits. Mean pore size 30µm, polyethylene 1 24000230µm Vyon F PE frits to fit Combinatorial Microlute 1.5 x 7.2mm frits 1,000 239007Combinatorial Microlute™ plate, unpacked with bottom frits. Mean pore size 10 µm, polyethylene 1 24005410µm Vyon T PE frits to fit Combinatorial Microlute 1.5 x 7.2mm frits 1,000 239010Empty 96-well MicroLute shell with no top or bottom frits 40 240011S O L I D P H A S E E X T R A C T I O NCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENT29W W W . M I C R O P L A T E S . C O M? Unique design eliminates mess associated with traditional manual SPE cartridge methods? Up to 4 times faster than cartridge methods? Constructed from a single piece of high quality polymer which will not bend or distort? A range of packed bed volumes ranging from 250mg, through to 1500mg? Capability to process up to 200ml of sample in one plate? Frit pore size of 20µm? Each well has an individual drain spout ensuring 100% sample transfer and zero cross-contamination? Manufactured within ANSI/SLASstandard to allow easy automationMaxiLute™The development of a 48-well solid-phaseextraction (SPE) system allows the stepbetween SPE cartridges and SPE 96-wellplates to be managed.With the larger packed bed volume andincreased sample volume, the design of theMaxiLute™ offers advantages for drugdevelopment, food and environmentallaboratories currently using SPE cartridges.Manufactured from virgin polypropylene andloaded with a choice of sorbents andvolumes.MaxiLute™Sorbentvolume 250mg 500mg 1000mg 1500mgSCX 322001 321001 318001 320001SAX 322002 321002 318002 320002Silica 322003 321003 318003 320003C18 322004 321004 318004 320004CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENT30S O L I D P H A S E E X T R A C T I O NW W W . M I C R O P L A T E S . C O MBiological samples commonly contain proteinsthat interfere with downstream applications.The P3 plate uses the CRASH method, in whichthe protein is denatured with acetronitrile andthe flocculant filtered out, allowing 96 samplesto be handled at one time. The Protein Precipitation Plate, P3, is based onthe industry standard MicroLute™ format, butwithout the chromatographic sorbent. Protein‘crashes’ out of solution and precipitatesdirectly in each well when acetonitrile is added,thus solving all common problems associatedwith the CRASH technique of protein clean-up.The novel dual frit, hydrophobically treatedmatrix means that there is no ‘wetting out’ andleakage of the sample through the plate beforethe application of vacuum. P3 is nowcommonly used as the protein precipitationplate of choice in many major pharmaceuticalcompanies.Optional drain cap and top cap mats areavailable for those wishing to use vortexsample mixing (see page 31).P3 Protein Crash Plates*The Protein MicroLute High Efficiency plate is exactly the same as the P3 but with thefrits untreated. This plate is used when sample and acetronitrile are pre-mixed beforebeing pipetted into the plate.? Dual frit design? Pre-filter frit at 100µm traps large flocculant particles? Secondary frit traps fine protein particles at <10µm? Frits are Hydrophobic/Oleophobic. This retains sample/acetonitrile in the well to allow precipitation of proteins until vacuum is applied.? Pore size optimized to allow ideal flow rate? Inert filter material to ensure no adsorption of sample components? Frit structure prevents break through of protein particles? Industry standard MicroLute™ format enables easy automation? Specially selected polypropylene for low extractables? Disposable reservoir tray. Used for the collection of waste products in the vacuum manifold? Sealing cap, square well, fits top of MicroLute™. Used for retaining the sample in the well during transit or stopping the well from becomingcontaminated? Drain cap mat that seals the bottomof the plate, preventing liquid leakageMicroLute™ Protein Precipitation PlateDescription Qty/pack Cat. no.P3 Protein Precipitation Plate (single pack) 1 240100P3 Protein Precipitation Plate (bulk pack) 5 240200P3 Protein Precipitation Plate High Efficiency* 1 240010CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENTMicroLute™ – accessoriesDescription Qty/pack Cat. no.Disposable reservoir tray 25 219010Sealing cap, square well, fits to top of plate 50 219004Drain cap mat, seals base of plate 25 219005? Disposable reservoir tray. Used for the collection of waste products in the vacuum manifold? Sealing cap, square well, fits top of MicroLute™. Used for retaining the sample in the well during transit or stopping the well from becoming contaminated? Drain cap mat that seals the bottom of the plate, preventing liquid leakageMicroLute™ accessoriesS O L I D P H A S E E X T R A C T I O NW W W . M I C R O P L A T E S . C O M31MicroLute™ starter packStarter pack 271023? Uses an industry standard SPE plate, MicroLute™? Sorbents from Varian? Packed bed volumes ranging from 10mg to 100mg? Allows up to 2ml of sample to be tested? Can be packed either in 8-row or 12-column format? Manufactured within ANSI/SLAS standard to allow for automationThe kit consists of:? 2 x 50mg MicroLute™ – containing: 50mg of Varian C18, C8, C2, SAX, SCX, Certify, Certify II and CBA,1 x 50mg Varian C18? 1 x acrylic manifold? 1 x 1ml spacer insert? 3 x 2ml 96-well microplates? 3 x disposable reservoir traysMicroLute™ – Development10mg 15mg 25mg 50mg 100mgEight sorbents 271014 271016 271018 271020 271022Twelve sorbents 271024 271026 271028 271030 271032Development MicroLute™This is an SPE Development MicroLute™, which is designedfor research and development laboratories, where it may notbe known which sorbent best suits the process.The system allows the researcher to specify which sorbents gointo specific wells, with what designated packed bed volume.This allows the researcher to have full control of the SPEplate.SPE starter packThis kit has been designed to form thestarting point for SPE analysis. This gives theresearcher the necessary hardware to processsamples using an SPE plate and to collectunder vacuum the analyte for further analysis.Vacuum manifoldsVacuum manifolds are used to draw liquidthrough a filter or SPE plate into either a wastetray or a collection plate. The application ofvacuum increases the speed at which samplescan be collected.MicroLute™ manifoldThe MicroLute™ vacuum manifold from PorvairSciences is precision machined from crystal clearacrylic (top plate) and acetal polymer (plenumchamber). The acrylic top plate allows visualaccess to the plenum chamber for checkingprogress of the separation process.? Designed to take most filter plates manufactured to the ANSI/SLAS specification with long drip directors? Also designed so that SPE plates can be used, especially the Porvair Sciences MicroLute™ plate? Fitted with valve controller to ensure accurate adjustments of vacuum to the manifold? On/off valve for speed of use? Square well collection plate volumes of 350µl, 1ml and 2ml may be used. Any plate up to 44mm in height can be used? Fitted with a custom O-ring in upper surface allowing airtight interface between plates during operation ? Removable top plate to install reservoir tray or collection plate? Chamber has a medium resistance to alcohols and weak acidsMicroLute™ manifoldDescription Qty/pack Cat. no.Standard MicroLute™ manifold to hold deep 96-well collection plate 1 228008Replacement gasket, profile (to fit between top plate and vacuum chamber), for 228008/228020 1 228007Replacement gasket, flat (to fit top plate below filtration plate), for 228008/228020 1 228009Optional spacer insert, polypropylene, to allow use of 1ml round well polypropylene microplates (219002)in acrylic deep well manifolds (228008/228020) 1 228010Optional spacer insert, HDPE, to allow use of 350µl microplates in acrylic manifolds (228008/228020) 1 228012Disposable reservoir tray, PVC 25 219010Single plate manifold, high impact polystyrene, for filter plates (no waste collection) (not shown) 1 228001E Q U I P M E N T – V A C U U M M A N I F O L D SCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION32W W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTIONE Q U I P M E N T – V A C U U M M A N I F O L D S33W W W . M I C R O P L A T E S . C O MUniversal roboticmanifoldBased on our successful MicroLute™ AcrylicManifold, the new Universal RoboticManifold is designed to be easily assembledand disassembled by robotic manipulators,thus enabling productive automation of SPEor DNA clean-up procedures. Able toaccommodate collection plates from 14mm -44mm in height and adaptable for workingwith short, medium and long skirts and dripdirectors, the Universal Robotic Manifold canoperate with many different brands of filterplate and collection trays. Supplied with anintegral vacuum gauge, the Manifold providescomplete control of vacuum pressure ensuringplates are processed with high reproducibility.The Manifold is fully compatible with mostcommercial robotic liquid handling systems.The Universal Robotic Manifold is suitable fora range of manufacturer’s plates without anadaptor. For certain plate types a ShallowAdaptor or a Deep Adaptor, is required.? Compatible with any filter plate type? Robotic friendly design aids automation? Chemically resistant acetal/acrylic construction? Adaptable for different collection plates? Easy visual inspection of process? Built in vacuum gauge for reproducibilityUniversal robotic manifoldDescription Qty/pack Cat. no.Universal robotic compatible manifold to hold deep 96-well collection plate 1 228020Adaptor 1 for medium skirt/medium drip director plates 1 228021Adaptor 2 for short skirt/long drip director plates 1 228022Gaskets and spacers are identical to those for our standard MicroLute™ acrylic manifold shown on page 43.Manufacturer Plate type Base Adapt. 1 Adapt. 2Qiagen™ ¦ ¦Waters™ Standard plate ¦Waters™ µElution plate ¦ ¦Biotage™ ¦Varian™ ¦Phenomenex™ ¦Axygen™ ¦ ¦Seahorse™ ¦Porvair™ ¦Microplate evaporatorsPorvair evaporators are designed to removethe traditional laboratory ‘bottleneck’ ofsolvent evaporation from microplates prior toanalysis or reconstitution in storage buffer.These evaporators give significant throughputadvantages to laboratories looking tooptimize microplate sample preparationproductivity. Faster than centrifugalevaporation, significant increases in samplethroughput are achieved through advancedevaporator head technology and aninnovative manifold design, which directlyinjects heated nitrogen into each individualwell of the microplate simultaneously. Theevaporators have been designed to be simpleto install, operate and maintain. Installationrequires only connection to a gas supply orcylinder and mains electricity. Safety ofoperation is ensured as the UL compliantmarked compact units fit into all fumecupboards. Not suitable for high boilingsolvents such as DMSO and water.Both MiniVap and UltraVap may be operatedwith a supply of clean, dry compressed air inplace of nitrogen, if the chemistry allows. Anin-line gas filter must be used in this case.MiniVap™The MiniVap is purpose-designed for low usageresearch and development departments wherelow numbers of individual plates or vials needdrying. The MiniVap is simple to operate andmaintain. Installation requires only connection to agas supply and standard mains socket. With manualcontrol of the needle depth, gas temperature andflow rate, it allows fine control and quicker dryingtimes than other standard methods.? Quicker dry down times than standard methods such as vacuum oven? Tests have shown that the MiniVap can evaporate 500µl of methanol in less than 6 minutes? Designed for any ANSI/SLAS 96-well plate? 24 vial head now available? Simple to install and operate? Easy adjustments of temperature, gas flow rates and needle depth into the wells? Compact footprint – fits all standard fume cupboardsE Q U I P M E N T – M I C R O P L A T E E V A P O R A T O R SCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION34W W W . M I C R O P L A T E S . C O MMiniVap 229201MiniVap 229205E Q U I P M E N T – M I C R O P L A T E E V A P O R A T O R SCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION35W W W . M I C R O P L A T E S . C O MSpares and consumables for Porvair evaporatorsDescription Qty/pack Cat. no.Replacement 24 Needle Head with straight for Ultravap & MiniVap 1 229409Replacement 96 Needle Head with spiral needles for Ultravap & MiniVap 1 229072Replacement 96 Needle Head with straight needles for Ultravap & MiniVap 1 229036Replacement 384 Needle Head with straight for Ultravap 1 229073Spare Needle Kit, Spiral, Set of 10 c/w Fitting Tool for Ultravap-96/MiniVap 1 229074Spare Needle Kit, Straight, Set of 10 c/w Fitting Tool for Ultravap™ 384 1 229075Spare Needle Kit, Straight, Set of 10 c/w Fitting Tool for Ultravap-96/MiniVap 1 229035Gasket for needle manifold 1 229048Disposable vial rack with clear bottom for 13.75mm glass vials x 24 5 229216Ultravap Instruction Manual (Spare) 1 229523MiniVap Instruction Manual (Spare) 1 229524Protective clear side panels to allow fume extraction, Ultravap, pair 1 500007Fume extraction duct adaptor 4” to Ultravap ovoid rear fitting 1 500008MiniVap™ blowdown sample concentratorDescription Qty/pack Cat. no.Blowdown Evaporator MiniVap™ (230 Volts), with 96 spiral needles 1 229201Blowdown Evaporator MiniVap™ (230 Volts), with 96 straight needles 1 229200Blowdown Evaporator MiniVap™ (230 Volts), with 24 straight needles 1 229205Blowdown Evaporator MiniVap™ (110 Volts), with 96 spiral needles 1 229203Blowdown Evaporator MiniVap™ (110 Volts), with 96 straight needles 1 229202Blowdown Evaporator MiniVap™ (110 Volts), with 24 straight needles 1 229204Blowdown Evaporator MiniVap™ (230 Volts), without a head 1 229206Blowdown Evaporator MiniVap™ (110 Volts), without a head 1 229207MiniVap375.1235.00230.90341.40439.00401.57363.10281.1067.10139.40151.50261.4010.00204.00198.00156.50ø 38.0035.00UltraVap RCE Q U I P M E N T – M I C R O P L A T E E V A P O R A T O R SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION36? Designed for heat sensitive and thermally labile samples? Nine different drying programs can be retained in the memory? Ability to upload and download methods? Robotic plate stage automatically moves plates up in two drying phases? RS232 allows remote control via PC or LH robot? Removes most chromatography solvents in just minutes? Can evaporate 500µl methanol in 5 minutes? Compact footprint allows the Ultravap to fit into all standard fume cupboards? Choice of straight, curved & 24, 96 or 384 needles? Compatible with Tecan™, Hamilton™ and Caliper™ robotic liquid handlersUltravap™ RCThe new Ultravap RC from Porvair Sciences isthe latest fully robot-compatible version ofour popular Ultravap nitrogen blow-downevaporator, suitable for integration within thesafety enclosure of several leading laboratoryliquid handling robots. Designed with thedemands of linear robots very much in mind,the Ultravap RC comprises a fullymicroprocessor controlled dry down stationfor 24-, 96- or 384-well plates, together withclear safety side screens and a 4 inch fumehood duct adaptor.Ultravap™ RC high speed sample concentratorDescription Qty/pack Cat. no.Ultravap RC robot compatible blowdown evaporator with 96 spiral needles, 230V 1 229070Ultravap RC robot compatible blowdown evaporator with 96 spiral needles, 110V 1 229080Ultravap RC robot compatible blowdown evaporator with 96 straight needles, 230V 1 229135Ultravap RC robot compatible blowdown evaporator with 96 straight needles, 110V 1 229136Ultravap RC robot compatible blowdown evaporator with 384 straight needles, 230V 1 229071Ultravap RC robot compatible blowdown evaporator with 384 straight needles, 110V 1 229081Ultravap RC robot compatible blowdown evaporator with 24 straight needles, 230V for 24 well plates and vials 1 229138Ultravap RC robot compatible blowdown evaporator with 24 straight needles, 110V, for 24 well plates and vials 1 229139Ultravap RC robot compatible blowdown evaporator without head, 110V 1 229146Ultravap RC robot compatible blowdown evaporator without head, 230V 1 229145Ultravap RC robot compatible blowdown evaporator, 384 type, without head, 110V 1 229082CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION37E Q U I P M E N T – M I C R O P L A T E E V A P O R A T O R SW W W . M I C R O P L A T E S . C O MNitrogen blow-downThe nitrogen blow-down principle is easy tounderstand. Warm gas is blown down intothe wells of the microplate, just above theliquid level. The effect is to speed up solventevaporation by providing more energy for thelatent heat of evaporation. This enables asteady-state equilibrium to be reached morequickly, leading to shorter drying times.Nitrogen blow-down has been shown to bethe easiest way to automate the frequentbottlenecks caused by the requirement toremove solvent from samples that need to beconcentrated, dried or reconstituted in a moresuitable solvent.Flexible programingThe Ultravap RC has been designed to allowrobots with extended gripper arms to placeand remove microplates directly onto theevaporation table. In comparison withprevious models, the Ultravap RC is22mm shorter at the front end, allowingbetter access for the gripper arms. Theevaporation table is able to rise under thecontrol of a stepper motor as the dryingprocess proceeds. This can be programed at asuitable rate for each solvent type beingevaporated. In addition, gas temperature,pressure and flow rate can all be programedindividually and stored in one of ninemultistep programs on the UltravapRC. Each program allows the tableto rise in two distinct ramped phases,so that a fast initial drying period canbe followed by a gentler final dryingphase.With the addition of the optionalclear acrylic side sheets and the 4inch fume duct adaptor, the newUltravap RC unit lends itself to fullintegration with Tecan and Hamilton roboticliquid handlers, where it is usually located onthe right-hand side of the deck. Controlcommands are sent directly from the robotcontroller to the Ultravap. These standardcommands are listed in the manual, but mostrobot manufacturers have drivers available tocontrol the Ultravap, making integration aseamless process.Solvent compatibilityWith a choice of 96 straight or 96 spiral needles, plus 24 and384 straight needles, the sphere of application for UltravapRC is huge. Most common chromatography solvents can beevaporated with ease, such as dichloromethane, methanol,acetonitrile and hexane. The nitrogen blow-down method isnot, however, suitable for high-boiling point solvents such asDMF, DMSO and water. It is also unable to be used with acidsor acid chlorides, as special corrosion-resistant systems areneeded for this work. The choice of straight or spiral needlesallows the user to choose between faster dry down (spiral)and better final drying in V-well plates (straight). The spiralscause a vortex to form in the solvent, increasing the surfacearea and thus speeding up the rate of evaporation. However,for plates with V-bottom wells, or for smaller wells such asthose in 384-well plates, straight needles are required.Evaporator system requirementsGas flow rate: 28L/min minimum, 80L/min maximum at 6 bar pressure.Can be used with nitrogen or dry compressed air if thechemistry allows.Not suitable for use with strong acids or acid chlorides.UltraVap RC 229135E Q U I P M E N T – M A T C A P P E R A N D D E - C A P P E RW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION38Mat CapperFor laboratories having to seal mediumnumbers of microplates the Mat Capperoffers an affordable solution. Compact andportable, the Mat Capper is very easy to use,requiring only one operation of the system toproduce an accurate and tight seal on a widerange of both collection and shallow wellmicroplates. The seals used are EVA or siliconemats, which work by friction fit and arere-usable with care. For a wide range offriction seals, please see page 44.MicroLute™ De-capperThe MicroLute™ De-capper is for use withany MicroLute™ plate fitted with a drain cap.It enables the quick and easy removal of theprotective lower drain cap, enabling the plateto be used.? Caps shallow and deep well polypropylene storage plates? Needs minimal pressure for capping, reducing fatigue and RSI? Powder coated to resist chemical spillage? Universal plate ‘shuttle’ to take shallow or deep well plates? Fixing holes for securing to benchMat Capper and MicroLute™ De-capperDescription Qty/pack Cat. no.Mat Capper, applicator for storage and assay plates 1 229078MicroLute™ De-capper, for use with drain caps (not shown) 1 229110Mat CapperShown with cap mat 229093Thermal plate sealersOf the various methods for sealingmicroplates, heat sealing has become thepreferred option. It creates an air tight andchemically-resistant seal without thecomplications of adhesives being applied to aplate. Porvair Sciences has developed a rangeof units to meet the needs of low, mediumand high throughput laboratories formicroplate sealing.MicroSeal manual thermal plate sealerThe MicroSeal is designed to becompact, easy to use andergonomic. Heat sealing a widerange of plates is quick andsimple. Heat sealing protectsprecious samples fromevaporation and contaminationwhen performing PCR or duringstorage. A built-in thermostatprevents overheating of theMicroSeal and with its smallfootprint and ease of use, thenew MicroSeal is the obviouschoice for manual sealing of PCRplates and microplates. With a pre-settemperature of 170ºC, ideal for mostcommon sealing applications, and dualLED status display for power and heating, theMicroSeal is safe and easy to use. Anergonomic pull down action allows singleaction sealing of most common plate types.Additional adaptors are available for specificplates.MicroSeal plate sealerDescription Qty/pack Cat. no.MicroSeal plate sealer 110/230v 1 229751Plate adaptor for ANSI/SLAS deep-well plates 1 229752Plate adaptor for rimless 96-well PCR plates 1 229753Plate adaptor for ANSI/SLAS standard 14mm plates 1 229754Plate adaptor block 384 deep well plates 30.2mm high 1 500014? Fast warm up time? Consistent, single action sealing? Pre-set temperature for ease of use? Sealing temperature 170°C? Warm up time < 10 minutesE Q U I P M E N T – H E A T S E A L E R SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION39E Q U I P M E N T – H E A T S E A L E R SW W W . M I C R O P L A T E S . C O MCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION40MiniSeal IIsemi-automatic heatsealerThe new MiniSeal II semi-automatic platesealer builds on the reputation of our earlierMiniSeal design for robustness and an abilityto seal most types of plate. For users whoneed a tight seal on deep well plates,conventional sealers may not offer sufficientdown force to guarantee a good seal.With powerful stepper motor control and amighty 450 watts of heating power available,the new MiniSeal II copes effortlessly withPCR, filter-bottomed, assay and deep wellplates, EVEN if the plate itself is distorted orbowed. Easy to set up with simple up/downprograming for both sealing time and sealingtemperature, the MiniSeal II also boasts arobust drawer design that ensures excellentconformance to health & safety regulations.With so much available power, sealing timesfor most polypropylene plates with Porvairseals are less than 3 seconds each. Sealedplates are automatically ejected from theMiniSeal II. With a footprint smaller than asheet of A4 paper and weighing just 6kgs,this electrically-driven sealer is ideal for smallbench spaces.Unlike some other manufacturer’s products,MiniSeal II comes complete with plateadaptors for standard SLAS microplates, deepwell microplates and PCR plates. The unusedadaptors are ingeniously designed to stack ontop of the unit when not in use. Also includedis a brass plate weight to keep your foils andseals flat during sealing.? Desktop semi-automatic Heat Applied Plate Sealing System? Seal SLAS format micro well plates and tubes? Seals plates of any height from 9 - 48mm? Temperature range from ambient to 200ºC? Seal time from 0.1 - 9.9 seconds? Simple slide operation? Combined temperature and seal time display? Light weight system, only 6kgs (14lbs)? 110V / 220V 50/60 Hz compatible? No compressed air requiredMiniSeal II semi-automatic single plate sealerDescription Qty/pack Cat. no.MiniSeal II semi-automatic single plate heat sealer 110V / 220V 1 500090Supplied complete with two plate adaptor blocks, plate weight and line cord.Skirtless plate adaptor 96-well 1 500083Plate adaptor flat top 384 PCR plate 1 500084CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION41E Q U I P M E N T – S E A L S A N D F O I L SW W W . M I C R O P L A T E S . C O MPorvair seals and foilsPorvair Sciences has a comprehensive rangeof seals and foils for thermal and adhesivesealing of microplates. In addition, a full rangeof re-usable cap mats is available for frictionsealing of plates.Foils and seals for the MicroSeal and MiniSealPlus are supplied as cut sheets, 125mm x78mm, in packs of 100. For the automatedTriSeal system, these come in roll form.Sample rolls are 5m long, whilst productionrolls are either 500m or 610m long, enoughto seal 5-6,000 plates on average.All Porvair heat seals have a sealing integrityrange of -80ºC to +120ºC, are resistant toDMSO and TFA as well as most other solventsand will seal polypropylene plates. Forpolystyrene plates, please only use Clear Seal.Adhesive seals (suitable for use down to -40ºC unless shown)Description Qty/pack Cat. no.SealPlate® clear adhesive film for PP or PS plates 100 229016ThermalSeal RT2™ thermal cycler film for real-time PCR polyester 50µm thickness 100 229301ThermalSeal RT5™ thermal cycler film for real-time PCR polyester 125µm thickness 100 229302ThermalSeal A® thermal cycler film for standard PCR 100 229303ThermalSeal A® thermal cycler film for standard PCR, sterile 100 229304ThermalSeal RT2RR™ thermal cycler film for real-time PCR – 50µm films 100 229521AlumaSeal II™ aluminium seal for PCR and storage down to -40°C 100 229305AlumaSeal 96® aluminium seal for PCR and storage in 96-well plates with raised rims 100 229497AlumaSeal 384™ aluminium seal for PCR and storage in 384-well rimless plates 100 229498AlumaSeal CS™ aluminium seal for long term cold storage -80°C 100 229499Absorb Max™ optically-black film for light-sensitive assays 100 229520EZ-Pierce™ easily pierceable polyethylene film 100 229306Aeraseal™ gas-permeable, hydrophobic Rayon seal for cell culture (-20ºC only) 100 229307Aeraseal™ gas-permeable, hydrophobic Rayon seal for cell culture, sterile (-20ºC only) 50 229308X-pierce™ pre-scored film 96 Well Round – sterile 50 229495X-pierce™ pre-scored film 96 Well Round 100 229496Heat seals for use with TriSeal or other automated sealersDescription Qty/pack Cat. no.All foils and films are 78mm width, sheet length is 125mmPeel-Seal peelable sealing foil 1 x 610m 2294201 x 5m 229450Pierce-Seal pierceable sealing foil 1 x 610m 2294211 x 5m 229451Clear Seal optically clear film for 1 x 500m 229422fluorescence/colorimetric assays 1 x 5m 229452100 sheets 229472Pierce-Seal easily pierced foil for PCR 1 x 610m 2294401 x 5m 229460Thermal bond strong peelable sealing foil 1 x 500m 2294421 x 5m 229462Clear Weld-Seal strong permanent seal 1 x 610m 2294411 x 5m 229461100 sheets 229481Gas-permeable Rayon seal for cell culture 1 x 200m 2294431 x 5m 229463100 sheets 229553Porvair colour-coded thermal sealsDescription Qty/pack Cat. no.Easily pierced 20µm PP/PS lacquered aluminium foil – colour coded green125mm x 78mm sheets printed with colour coding and right side up for ease of use 100 229572Peelable 70µm polyester/alumninium laminate sealing foil – colour coded red125mm x 78mm sheets printed with colour coding and right side up for ease of use 100 229571Pierceable/peelable 38µm lacquered aluminium foil – colour coded blue125mm x 78mm sheets printed with colour coding and right side up for ease of use 100 229573Strong 85µm thick aluminium foil for long term storage – colour coded black125mm x 78mm sheets printed with colour coding and right side up for ease of use 100 229574Sealing film optically clear 75µm – non peelable, but pierceable125mm x 78mm sheets printed with black coding and right side up for ease of use 100 500009Sealing film optically clear 105µm – peelable, but non pierceable125mm x 78mm sheets printed with black coding and right side up for ease of use 100 500010CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION42W W W . M I C R O P L A T E S . C O ME Q U I P M E N T – S E A L S A N D F O I L SAdhesive seals229494Porvair MicroBurst™Microencapsulated clearsealing filmUsing adhesive seals whilst wearing laboratorygloves can prove to be difficult. The glued surfaceof the seal can easily stick to your glove rather thanyour microplate. Porvair MicroBurst™ filmseliminate this problem. When first handled,MicroBurst™ is not tacky and will not stick toanything. Only when you are satisfied that the filmis in the correct place do you press down hard toactivate the MicroBurst™ adhesive. Millions of tinyspheroids contain the adhesive and these“microcapsules” burst under applied pressure torelease the adhesive just where you want it andnot all over your glovesThe patented MicroBurst™ technology is beingoffered for the first time in a Life Sciencelaboratory by Porvair Sciences. The clear film hasexcellent optical properties and once activated,produces a very firm seal which quickly strengthsto form a near-permanent seal on your plates.MicroBurst™ film can be pierced by pipette tips orrobot liquid handlers and is temperature stable –making it ideal for PCR work.MicroBurst™ non-sticky adhesive sealDescription Qty/Pack Cat. No.Microburst™ clear seal with microencapsulated adhesive coating 100 229731Adhesive film application paddle 5 229732Adhesive film application roller 1 229733229016Zone-Free™ adhesive sealDescription Qty/Pack Cat. No.EZ-Pierce™ sealing film 96-well round sterile 50 229493EZ-Pierce™ sealing film 96-well round 50 229494CUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION43E Q U I P M E N T – S E A L S A N D F O I L SW W W . M I C R O P L A T E S . C O MHeat seals and foilsHeat seals and foilsFriction sealing strips and PCR cap mats229472229443229481229572 229571229574229573360005286113286116E Q U I P M E N T – S E A L S A N D F O I L SCUSTOM MANUFACTURELIFE SCIENCESSTORAGE/COLLECTIONSOLID PHASE EXTRACTION44W W W . M I C R O P L A T E S . C O MFriction seals229090229091229093219004219003360006219004Friction seals (cap mats)Description Cross reference to Porvair Sciences plates Qty/pack Cat. no.Silicone sealing mat to fit 384-well plates 219040, 219041 50 380001EVA sealing mat to fit 96-square well plates 219006, 219008, 219009 50 219004EVA sealing mat to fit 96-square well plates, sterile 219025, 219026, 219027 50 219019Pierceable Santoprene mat for 96-square well plates 219006, 219008, 219009 100 360010EVA sealing mat for 96-round well plates 219002, 219032 50 219036EVA sealing mat for 96-round well plates, black 219002, 219032 50 219038EVA sealing mat for 96-round well plates, sterile 219012 50 219042Pierceable Santoprene seal strip to fit 24-well plates 360013 100 360014Pierceable Santoprene seal mat to fit 48-well plates 360002, 360004 100 360006Pierceable Santoprene seal strip to fit 96-well plates 360001 400 360007Pierceable sealing cap, round 96-well 219002 50 219044Mat for round 96-well plates (standard silicone) 219002 and round well plates 5 229090Mat for square 96-well plates (standard silicone) 229230 and square well plates 5 229091Mat for round 96-well plate (PTFE coated silicone) 219002 and round well plates 5 229092Mat for square 96-well plate (PTFE coated silicone) 229230 and square well plates 5 229093Polystyrene clear lid for all 96-well SBS standard plates Fits all plates 100 229125Polypropylene lid for all 96-well SBS standard plates Fits all plates 100 229128Cap mat for 1.1ml low profile storage plate 219250 5 21925196-well PCR sealing mat, natural For rimless PCR plates 50 28611396-well PCR sealing mat, rubber, natural For rimless PCR plates 50 2861148-well PCR sealing strip, 125 strips per pack, 10 packs per case – 1250 286116219251Custom manufacturePorvair Sciences has a long history ofsuccessfully helping customers develop newand innovative microtitre plates andequipment. Porvair Sciences expertise inpolymer molding, surface treatment,specialist assembly and general understandingof life science applications makes thecompany ideal for custom manufacture. Wecan also undertake ‘private labeling’ of ourstandard products. Current customers forcustom manufacture include the followinggroups:Porvair Sciences offers rapid solutions toproblems by consultancy, design and speedyprototyping for biotech, pharmaceutical andlife science companies.Techniques which Porvair Sciences use in thegeneration of custom products include:? Scientific instrument companies? Pharmaceutical R&D laboratories? Cell biology companies? Compound library suppliers? Diagnostic companies? Military contractors? Microarray research companies? Polymer ultrasonic welding? Plasma surface treatment of polymers? ‘Two-shot’ injection moulding? Co-sintering of polymers/silicasC U S T O M M A N U F A C T U R EW W W . M I C R O P L A T E S . C O MLIFE SCIENCESSTORAGE/COLLECTIONEQUIPMENTSOLID PHASE EXTRACTION45C H E M I C A L R E S I S T A N C E T O M E M B R A N E S A N D P L A T E M A T R I C E S46 W W W . M I C R O P L A T E S . C O MAcetaldehyde ? ? ? ? ? ¦ ? ?Acetic acid (glacial),100% ? ¦ ? ? ? ? ? ¦Acetic acid, 96% ? ? ¦ ¦ ? ? ? ?Acetic anhydride ¦ ¦ ? ? ¦ ¦ ? ?Acetone ? ? ? ? ? ? ? ¦Acetonitrile ? ¦ ? ? ? ¦ ? ¦Acetophenone ¦ ¦ ? ? ¦ ¦ ? ?Acetylacetone ? ? ? ? ?Acetylchloride ? ? ? ? ?Acrylic acid ? ? ? ? ?Acrylonitrile ¦ ? ? ? ? ? ? ?Adipic acid ? ? ? ? ? ? ? ?Allyl alcohol ? ? ? ¦ ? ? ? ?Aluminum chloride ? ? ? ? ? ? ? ?Aluminum hydroxide ? ? ¦ ¦ ? ? ? ?Amino acids ? ? ? ? ? ? ? ?Ammonium chloride ? ? ? ? ? ? ? ?Ammonium fluoride ? ? ? ? ? ? ? ?Ammonium hydroxide,30% (Ammonia) ? ? ¦ ? ? ? ? ?Ammonium sulfate ? ? ? ? ? ? ? ?n-Amyl acetate ¦ ? ? ? ? ¦ ¦ ?Amyl alcohol (Pentanol) ? ? ¦ ¦ ? ? ? ?Amyl chloride(Chloropentane) ? ? ? ? ? ? ? ?Aniline ? ? ? ? ? ? ? ¦Barium chloride ? ? ? ? ? ? ? ?Benzaldehyde ? ? ? ? ? ? ? ?Benzene (Benzol) ? ¦ ? ? ? ? ¦ ?Benzine (Gasoline) ¦ ¦ ? ? ? ? ¦ ?Benzoyl chloride ? ¦ ? ? ? ? ¦ ?Benzyl alcohol ¦ ? ? ? ¦ ? ¦ ?Benzylamine ¦ ? ? ¦ ¦ ?Benzylchloride ? ?Boric acid, 10% ? ? ? ? ? ? ? ?Bromine ? ? ? ? ? ? ? ?Bromobenzene ? ? ? ? ? ? ? ?Bromoform ? ? ? ? ? ? ? ?Bromonaphthalene ? ?Butanediol ? ? ? ? ? ? ? ?1-Butanol ? ? ¦ ? ? ? ? ?Butylamine ? ?n-Butyl acetate ¦ ¦ ? ? ? ? ¦ ¦Butyl methyl ether ? ¦ ? ? ¦ ? ¦ ?Butyric acid ? ? ? ? ¦ ? ? ?Calcium carbonate ? ? ? ? ? ? ? ?Calcium chloride ? ? ? ? ? ? ? ?Calcium hydroxide ? ? ? ¦ ? ? ? ?Calcium hypochlorite ? ? ? ? ? ? ? ?Calomel ? ? ? ¦ ? ? ? ?Carbon disulfide ? ? ? ? ? ? ? ?Carbon tetrachloride ? ? ? ? ¦ ? ? ?Chloroacetalaldehyde,45% ? ?Chloroacetic acid ? ? ? ? ? ? ? ?Chloroacetone ? ?Chlorobenzene ¦ ? ? ? ? ? ? ?Chlorobutane ¦ ? ? ? ¦ ? ¦ ?Chloroform ? ? ? ? ? ¦ ? ?Chromic acid, 10% ? ? ? ? ? ? ? ?Chromic acid, 50% ¦ ¦ ? ? ? ¦ ? ¦Chromosulfuric acid ? ? ¦ ¦ ? ? ? ?Copper sulfate ? ? ? ? ? ? ? ?Cresol ¦ ¦ ? ? ¦ ? ? ?Cumene(isopropyl benzene) ¦ ? ? ? ? ¦ ¦ ?Cyclohexane ¦ ? ? ? ¦ ? ¦ ?Cyclohexanone ¦ ? ? ? ¦ ? ? ?Cyclopentane ¦ ? ? ? ¦ ? ? ?Decane ¦ ¦ ?1-Decanol ? ¦ ?Dibenzylether ? ? ? ?Dibutyl phthalate ? ¦ ? ? ¦ ? ¦ ?Dichloroacetic acid ¦ ? ¦ ? ¦ ¦ ¦ ?Dichlorobenzene ¦ ? ? ? ¦ ? ¦ ?Dichloroethane ¦ ? ¦ ? ¦ ?Dichloromethane ¦ ? ? ? ¦ ? ¦ ?Diesel oil (Heating oil) ? ¦ ? ? ? ¦ ¦ ?Diethanolamine ¦ ? ? ¦1.2 Diethylbenzene ? ? ? ? ¦ ? ? ?Diethylether ¦ ? ? ? ¦ ? ? ?Diethylamine ¦ ? ¦ ¦ ¦ ? ? ?Diethylene glycol ? ? ¦ ? ? ? ? ?Dimethyl sulfoxide(DMSO) ? ? ? ? ? ? ? ?Dimethylaniline ? ?Dimethylformamide(DMF) ? ? ? ? ? ? ? ?1.4 Dioxane ? ¦ ? ? ? ? ? ¦PP PS PE-HD PE-LDChemical 20°C 50°C 20°C 50°C 20°C 50°C 20°C 50°CPP PS PE-HD PE-LDChemical 20°C 50°C 20°C 50°C 20°C 50°C 20°C 50°CThe following table has been compiled from data gathered from varioussources and is intended as a guide only.? High resistanceNo damage to plastic after 30 days of contact with medium. The plasticcould remain resistant for much longer – perhaps years.¦Medium resistanceProbability of some damage to plastic after 7 – 30 days, although sucheffects as discoloration, reduced mechanical firmness, softening andswelling may be partially reversible.? Low resistanceImmediate damage to plastic may occur as a result of contact with medium,including cracks, deformation, discoloration, dissolution and reducedmechanical firmness. Not suitable for constant contact with medium.47W W W . M I C R O P L A T E S . C O MC H E M I C A L R E S I S T A N C E T O M E M B R A N E S A N D P L A T E M A T R I C E SDiphenyl ether ? ?Ethanol ? ? ? ? ? ? ? ?Ethanolamine ?Ethyl acetate ? ¦ ? ? ? ? ? ?Ethyl methyl ketone ? ¦ ? ? ¦ ? ¦ ?Ethylbenzene ? ? ? ? ? ? ? ?Ethylene glycol (glycol) ? ? ? ? ? ? ? ?Ethylene oxide ¦ ? ? ? ¦ ¦ ¦ ¦Fluoroacetic acid ? ?Formaldehyde, 40% ? ? ? ? ? ? ? ?Formamide ? ? ? ? ? ?Formic acid, 100% ? ? ? ¦ ? ? ? ?Glycerol ? ? ? ? ? ? ? ?Glycolic acid, 50% ? ? ? ? ? ?Heating oil (Diesel oil) ? ¦ ? ? ? ¦ ¦ ?Heptane ¦ ¦ ? ? ¦ ¦ ¦ ?Hexane ? ¦ ¦ ? ? ¦ ¦ ?Hexanol ? ? ? ? ? ?Hydriodic acid ? ? ? ? ? ?Hydrobromic acid ? ? ¦ ? ? ? ? ?Hydrochloric acid, 10% ? ? ? ? ? ? ? ?Hydrochloric acid, 20% ? ? ? ? ? ? ? ?Hydrochloric acid, 37% ? ? ¦ ¦ ? ? ? ?Hydrofluoric acid, 40% ? ? ? ? ? ? ? ?Hydrofluoric acid, 70% ? ¦ ? ? ? ¦ ? ?Hydrogen peroxide, 35% ? ? ? ? ? ? ? ?Isoamyl alcohol ? ? ? ? ? ?Isobutanol ? ? ¦ ¦ ? ? ? ?Isooctane ¦Isopropanol (2-propanol) ? ? ¦ ¦ ? ? ? ?Isopropyl ether ? ? ? ? ? ? ? ?Lactic acid ? ? ? ? ? ? ? ?Lugol‘s solution (iodine-potassium iodide solution) ? ? ¦ ? ? ? ? ?Mercury ? ? ? ? ? ? ? ?Methanol ? ? ¦ ? ? ? ? ¦Methoxybenzene ? ?Methyl formate ? ?Methyl propyl ketone ? ¦ ? ? ? ? ? ¦Methylene chloride ¦ ? ? ? ¦ ? ¦ ?Mineral oil (engine oil) ? ? ? ? ? ? ¦Monochloroacetic acid ? ? ? ? ? ? ? ?Nitric acid, 10% ? ? ? ? ? ? ? ?Nitric acid, 30% ¦ ? ? ? ¦ ? ¦ ¦Nitric acid, 70% ? ? ? ? ? ? ? ?Nitrobenzene ? ? ? ? ¦ ? ? ?Nitrohydrochloric acid ¦ ? ¦ ? ? ? ? ?Oleic acid ? ?Oxalic acid ? ? ? ? ? ? ? ?Ozone ¦ ? ¦ ¦ ¦ ? ¦ ?Perchloric acid ? ? ? ? ? ? ? ?Perchloroethylene ? ? ? ? ? ? ? ?Petroleum ¦ ? ? ? ¦ ? ¦ ?Petroleum ether ? ? ¦Phenol ? ? ? ? ? ? ? ¦Phenylethanol ¦ ¦Phenylhydrazine ¦ ¦Phosphoric acid, 85% ? ? ? ¦ ? ? ? ?Piperidine ? ?Potassium chloride ? ? ¦ ¦ ? ? ? ?Potassium hydroxide ? ? ¦ ¦ ? ? ? ?Potassium permanganate ? ? ? ? ? ? ? ?Propanol ? ? ¦ ? ? ? ?Propionic acid ? ¦ ¦ ? ? ¦ ¦ ?Propylene glycol(propanediol) ? ? ? ? ? ? ? ?Pyridine ¦ ¦ ? ? ? ¦ ? ¦Salicylaldehyde ? ? ? ? ? ? ? ?Salicylic acid ? ? ? ? ? ? ? ?Silver acetate ? ? ¦ ¦ ? ? ? ?Silver nitrate ? ? ¦ ¦ ? ? ? ?Sodium acetate ? ? ? ? ? ? ? ?Sodium chloride ? ? ? ? ? ? ? ?Sodium dichromate ? ? ? ¦ ? ? ? ?Sodium fluoride ? ? ? ? ? ? ? ?Sodium hydroxide, 30% ? ? ? ? ? ? ? ?Sulfuric acid, 60% ? ? ? ? ? ? ? ?Sulfuric acid, 98% ? ? ? ? ¦ ? ¦ ?Tartaric acid ? ? ? ? ? ? ? ?Tetrahydrofuran (THF) ¦ ? ? ? ¦ ? ¦ ?Toluene ¦ ? ? ? ¦ ¦ ¦ ?Trichloroacetic acid ¦ ? ¦ ? ¦ ¦ ¦ ?Trichlorobenzene ? ? ? ? ? ? ? ?Trichloroethane ? ? ? ? ¦ ? ? ?Trichloroethylene ? ? ? ? ¦ ? ? ?Trichlorotrifluoroethane ? ?Triethanolamine ? ?Triethylene glycol ? ? ? ¦ ? ? ? ?Trifluoroacetic acid (TFA) ? ?Trifluoro ethane ? ?Tripropylene glycol ? ? ? ? ? ? ? ?Turpentine ? ? ? ? ¦ ? ¦ ?Urea ? ? ? ? ? ? ? ?Xylene ? ? ? ? ¦ ? ¦ ?Zinc chloride, 10% ? ? ? ? ? ? ? ?Zinc sulfate, 10% ? ? ? ? ? ? ? ?PP PS PE-HD PE-LDChemical 20°C 50°C 20°C 50°C 20°C 50°C 20°C 50°CPP PS PE-HD PE-LDChemical 20°C 50°C 20°C 50°C 20°C 50°C 20°C 50°CThe information contained in this table is based on current knowledge, butits accuracy is not guaranteed and neither should it be regarded as offeringassurance of particular properties, or the suitability of products supplied byPorvair Sciences for specific purposes. 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