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Last updated: March 2013 © 2013 MicruX Fluidic, S.L. All Rights Reserved MicruX develops reusable microfluidic electrophoresis chips manufactured on highly resistant hybrid SU-8/Pyrex material. Microfluidic chips are fabricated with integrated electrodes or microelectrodes on the Pyrex cover plate. Two crossed microchannels fabricated on EPON SU-8 resin with integrated electrodes on Pyrex cover plate. Highly resistant hybrid SU-8/Pyrex material for reusable microfluidic chips. Long life (over 1000 runs/injections) with a low-cost, high efficiency and precision. Electrodes are integrated at the outlet of the separation channel with an end-channel approach. :: 50/150 nm titanium/platinum thin-film. :: 50/150 nm chromium/gold thin-film.WE: working electrode. RE: reference electrode. AE: auxiliary electrode. Electrodes are available in different designs and materials: *Customized designs of microfluidic chips and electrodes are available on demand. Separation of neurotransmitters dopamine (DA) and DOPA performed using a SU-8/pyrex microchip (ref. MCE-SU8-Pt001T) in combination with the microfluidic platform (ref. MCE-HOLDER-SC01) and MicruX® HVStat instrument (ref. HVSTAT2010). Electropherogram for the separation of 100µM DA and 500µM DOPA using a SU-8/pyrex single-channel microchip. Conditions: Running buffer: 25mM MES-His pH = 6.0; Vinj = +750V for 5s, Vsep = +1000V, Ed = +0.75V (vs. Pt) 0123456780 10 20 30 40 50 60 70 80Current (nA)time (s)DADOPA Two parallel microchannels fabricated on EPON SU-8 resin with integrated electrodes on Pyrex cover plate. Electrodes are integrated at the outlet of the separation channel with an end-channel approach. Two parallel working electrodes for simultaneous measurements. :: 50/150 nm titanium/platinum thin-film. WE: working electrode. RE: reference electrode. AE: auxiliary electrode. SU-8/Pyrex microchips are also provided with integrated microelectrodes on the pyrex cover plate. The working electrode is available in two different configurations: microelectrode array (MEA) and interdigitated array (IDA). Two crossed microchannels fabricated on EPON SU-8 resin with integrated microelectrodes on Pyrex cover plate. Highly resistant hybrid SU-8/Pyrex material for reusable microfluidic chips. Long life (over 1000 runs/injections) with a low-cost, high efficiency and precision. The electrochemical detection (ED) system consists of six 10-µm microelectrodes with an array approach. Electrodes integrated at the outlet of the separation channel with an end-channel approach. A 10-µm gap is shown between microelectrodes of array (WE). :: 50/150 nm titanium/platinum thin-film. :: 50/150 nm chromium/gold thin-film.WE: working electrode. RE: reference electrode. AE: auxiliary electrode. Electrodes are available in different materials: The electrochemical detection (ED) system consists of two arrays with three 10-µm microelectrodes with an interdigitated approach. Electrodes integrated at the outlet of the separation channel with an end-channel approach. A 10-µm gap is shown between microelectrodes of interdigitated array (WE1/WE2). :: 50/150 nm titanium/platinum thin-film. :: 50/150 nm chromium/gold thin-film.WE1/WE2: working electrodes. RE: reference electrode. AE: auxiliary electrode. Electrodes are available in different materials: Separation of phenolic compounds performed using a SU-8/pyrex microchip with integrated IDA microelectrode (ref. MCE-SU8-IDA-Pt005T) in combination with the microfluidic platform (ref. MCE-HOLDER-DC02) and MicruX® HVStat instrument (ref. HVSTAT2010). Electropherograms for the separation of 100µM DA, 100µM pAP and 250µM APAP using a SU-8/pyrex single-channel microchip with an IDA microelectrode applying same and different detection potentials. Conditions: Running buffer: 20mM MES pH = 6.0; Vinj = +750V for 3s, Vsep = +1000V. 024681012140 10 20 30 40 50 60Current (nA)time (s)DApAPAPAPWE2: +0.8VWE1: +0.8V-8-6-4-20246810120 10 20 30 40 50 60Current (nA)time (s)DApAPAPAPWE2: -0.25VWE1: +0.8V MicruX develops metal-based (micro)electrodes fabricated by thin-film technologies on a Pyrex substrate. A SU-8 resin protective layer is used to delimit the electrochemical cell enabling the use of very small sample volume. » Standard dimensions: 10 x 6 x 0.75 mm » Substrate: Pyrex » Protective layer: SU-8 resin » Electrochemical cell: 2 mm Ø » Sample volume: 1 – 5 µL » Electrode material: Platinum or Gold Thin-film electrochemical sensors are provided on a plastic strip for easy-handling. The electrochemical sensors show excellent features for electroanalytical applications, including the development of (bio)sensors. » Strip dimensions: 35 x 10 x 2 mm Electrochemical sensors are available in different standard designs and materials. Electrochemical sensors SE are based on a three-electrode (working - WE, reference - RE and auxiliary - AE) approach. The three electrodes are fabricated in the same material (platinum or gold) with high precision and resolution. Thin-film metal-based electrodes show an excellent electrochemical behavior with a good intra- and inter-electrode reproducibility. Cyclic voltammograms for 1 mM K4Fe(CN)6 in 0.1 M KCl at different thin-film Pt electrodes (ED-SE1-Pt). v = 50 mV/s, n = 5, RSD = 4% Cyclic voltammograms for 1 mM ferrocene methylalcohol in 0.05 M H2SO4 at different thin-film Au electrodes (ED-SE1-Au). v = 50 mV/s, n = 4, RSD = 6% Thin-film technologies enable the fabrication of microelectrodes (<25 µm) with high resolution and precision. Interdigitated array (IDA) electrodes take advantages of the microelectrodes features enhancing the sensitivity and detection limits. The working electrode consists of two individually addressable arrays of microelectrodes with an interdigitated approach. Thin-film IDA electrodes could be used in single- or dual-mode. Dual-mode enables the microelectrode behavior, reaching the steady-state in a short time and enhancing the analytical signals. Cyclic voltammograms for 1 mM K4Fe(CN)6 in 0.1 M KCl using single- and dual-mode at a Platinum IDA electrode. Cyclic voltammograms for 1 mM ferrocene methylalcohol in 0.1 M H2SO4 using single- and dual-mode at a Gold IDA electrode. Thin-film technologies open the gate to develop special microelectrodes designs. Interdigitated ring array (IDRA) electrodes bring a radial design specially focused on flow systems. The working electrode consists of two radial arrays of microelectrodes with an interdigitated approach. Dual-mode enables the microelectrode behavior, reaching the steady-state in a short time and enhancing the analytical signals Cyclic voltammograms for 1 mM K4Fe(CN)6 in 0.1 M KCl using single- and dual-mode at a Platinum IDRA electrode. Cyclic voltammograms for 1 mM ferrocene methylalcohol in 0.1 M H2SO4 using single- and dual-mode at a Gold IDRA electrode. An additional instrumentation such as high-voltage power supplies and potentiostat is required to use the microfluidic chips and electrochemical sensors. MicruX manufactures a new generation of instruments for using microfluidic electrophoresis chips with integrated electrochemical detection as well as other microfluidic devices. iHVStat (ref. iHVSTAT2012) brings the new evolution of electrophoresis systems based on microfluidic chips with amperometric detection. Dimensions: 165 x 150 x 95 mm (L x W x H). Battery-powered (LiPo – 3300 mAh). Control PC software. Interfacing: Serial RS232/ USB Adapter/ wireless (Bluetooth®). LED indicators: power, Bluetooth®, cable. HVStat (ref. HVSTAT2010) was the first microfluidic electrophoresis system that combined in a portable equipment a high voltage power supply and a bipotentiostat for dual amperometric detection. In this model, external cables are used for connecting the microfluidic platform with microchips electrophoresis. Dimensions: 165 x 150 x 85 mm (L x W x H). Battery-powered (LiPo – 3300 mAh). Control PC software. Interfacing: Serial RS232/ USB Adapter/ Bluetooth®. LED indicators: power, Bluetooth®, cable. Power: 1W Channels/ outputs: 1/ 4 Outputs polarity: Positive/negative Output voltage: ±3000V Max. output current: 0,34mA Ripple: < 1% Operating temperature: -20ºC to +70ºC Storage temperature: -20ºC to +105ºC Humidity: 20% to 85% RH DC-potential range: ± 2,00V DC-potential resolution: 1mV DC-offset error: ± 1mV Accuracy: = 0,1% Current ranges: 1nA to 1µA (4 ranges) Maximum current: ± 2µA Current resolution: 0,1% of current range / 1pA on lowest current range Electrochemical techniques: DC amperometric detection (AD) Pulsed amperometric detection (PAD) Run time: 1s - 8 (Experiments 1s – 1000h) Interval time: 10ms - 1000s (RS232/ USB) Pulse time: 5ms - 1000s (RS232/ USB) Maximum number of points: No limited (depending of computer memory) MicruX® HVStat & iHVStat instruments are provided in a suitcase with all the necessaries accessories for connecting it to a PC and using the microfluidic devices. MicruX Manager is a graphical user interface (GUI) to control the high voltage power supply and bipotentiostat of HVStat & iHVStat. MicruX Manager main interface for single- and dual-mode detection MicruX Manager experiment window with basic and advanced options MicruX Manager results window The platform DC (ref. MCE-HOLDER-DC02) is a perfect complement for using in combination with MicruX® HVStat & iHVStat. The microfluidic platform is the most new friendly interface for easy use of single- & dual-channel microchips with integrated electrochemical detection (including MEAs and IDAs). » Dimensions: 100 x 65 x 15 mm (L x W x H). » Material: black-methacrylate. » Integrated wells (buffer solution, sample, waste and detection reservoir) with standard fluidic ports (1/4”-28 UNF thread). » High voltage electrodes: Platinum (300 µm Ø) integrated on the cover. » Electrical contacts for detection and voltage electrodes on integrated PCB. » Integrated 2-mm female bananas for instruments connection. » Reusable. » It can be used with single & dual-channel microchips (38 x 13 mm) with integrated electrodes for single- & dual-mode amperometric detection. The holder SC (ref. MCE-HOLDER-SC01) is the basic interface for using single-channel microchips with integrated electrochemical detection. » Dimension: 90 x 48 x 15 mm. » Integrated wells (buffer solution, sample, waste and detection reservoir). » High voltage electrodes: Platinum (300 µm Ø) included on the cover (top part). » Electrical contacts for detection and voltage electrodes on integrated PCB. » Reusable. » It can be used with single-channel microchips 38 x 13 mm (ref. MCE-SU8-xx00XT). MicruX develops laboratory practice protocols for using the miniaturized instrumentation as teaching tool. These practices are focused on Analytical Chemistry Practical Courses (clinical, pharmaceutical, environmental, food and beverage analysis) for undergraduate & postgraduate students. The aim of these practices is to increase the knowledge of students about principles and applications of capillary electrophoresis technique as well as electrochemistry. Different packs are available for each practice, from a full- to a basic-pack, in order to fulfill the particular necessities of all educational centers. The most complete pack includes all the tools necessaries for introducing the students in the use of miniaturized instrumentation and microfluidic devices. » MicruX® HVStat (or iHVStat for iPack) Instrument + Accessories (Ref. HVSTAT2010 or iHVSTAT2012). » Microchip Holder DC series (Ref. MCE-HOLDER-DC02). » Microchips electrophoresis with integrated electrodes (Ref. MCE-SU8-Pt001T). » Teacher’s guide: includes detailed description of the experiments with the most relevant theoretical and practical aspects. » Student’s guide: includes a brief description about the main outlines of the experiments to be performed. » Excel Template: for collecting the experimental data and studying the results. » Reagents: specific chemicals for preparing the standard and buffer solutions. » Other material (syringes, filters, sample containers…) necessary for carrying out the experiments. » Microchips electrophoresis (Ref. MCE-SU8-Pt001T). » Teacher’s guide. » Student’s guide. » Excel Template. » Reagents. » Other material (syringes, filters, sample containers…). » Teacher’s guide. » Student’s guide. » Excel Template. » Reagents. » Other material (syringes, filters, sample containers…). » Reagents. » Other material (syringes, filters, sample containers…). Several practical protocols are being developed in order to involve different relevant fields such as health, environment, food and beverage. Analysis of uric acid and related compounds in urine sample using ME-ED Analysis of paracetamol and vitamin C in pharmaceuticals using ME-ED MicruX provides any additional complementary tools as well as reagents necessaries to suitable work with microfluidic devices, electrochemical sensors and portable analytical instrumentation. » Ref. ED-CONN-BOX Simple and universal connector for an easy starting test of the thin-film (micro)electrodes with the potentiostat. The electrode connector is compatible with any commercial potentiostat. Dimensions: 60 x 40 x 20 mm » Ref. MXF-HVCAB High voltage cable for using in combination with MicruX® HVStat to connect the HV electrodes. Cable dimensions: 50 cm long » Ref. MXF-BIPOTCAB Bipotentiostat cable for using in combination with MicruX® HVStat to connect the electrodes of detection system. Cable dimensions: 50 cm long » Ref. MXF-MES Buffer substance provided as single dose specially developed to carry out electrophoresis separations in microfluidic devices with electrochemical detection. Buffer is available in different pH between 5.0 and 7.0.