Proteomics - Targeted Mass Spectrometry (MRM/SRM) Services - Brochure

Multiple reaction monitoring (MRM, also known as Selective Reaction Monitoring – SRM) is a highly specific and sensitive mass spectrometry technique that can selectively quantify compounds within complex mixtures. This technique uses a triple quadrupole MS that firstly targets the ion corresponding to the compound of interest with subsequent fragmentation of that target ion to produce a range of daughter ions. One (or more) of these fragment daughter ions can be selected for quantitation purposes. Only compounds that meet both these criteria, i.e. specific parent ion and specific daughter ions corresponding to the mass of the molecule of interest are isolated within the mass spectrometer. By ignoring all other ions that flow into the mass spectrometer the experiment gains sensitivity, whilst maintaining exquisite accuracy.