SFR (Shake Flask Reader) Brochure

SFR Shake Flask ReaderOxygen and pH monitoring inErlenmeyer flasks Higher quality in your seed trainOptimization of growth conditionsCompatible with standard shakersFor microbial cultivations & cell culture?????????? ??SFR Shake Flask ReaderSystemsThe SFR Shake Flask Reader monitors oxygen and pH inup to 9 Erlenmeyer flasks simultaneously. It fits innearly all standard shakers. The  correspondingready-to-use flasks contain pre-calibrated sensor spots.The system monitors non-invasively through thetransparent bottom of the shake flask. Different typesand sizes of Erlenmeyers are available. Plastic flaskscontain pre-calibrated oxygen and pH sensors. They areready-to-use for one cultivation. Glass flasks areequipped with autoclavable oxygen sensors only. FeaturesRapid, parallel online monitoring of up to 63 shake flasksFor microbial cultivation and cell culturePre-calibrated shake flasks are ready-to-useCompatible with standard shakersGlass & plastic flasks in different sizes availableNon-invasive measurementUsed in seed train & bioprocess developmentSoftwareThe SFR Software SFRS is the integrated control center for the SFRShake Flask Reader. In addition, it offers an integrated database toshow and evaluate measurements. The connection between PC andSFR is wireless. Oxygen and pH are visualized in real-time during theentire cultivation. Results are displayed in a variety of graphicalrepresentations. All measured data can be exported to Excel® or as.csv for further evaluation. In addition, the current measurementcan be compared to stored cultivations online. BenefitsEnables process monitoring in shake flasksSystematic optimization of cultivation parametersReliable comparison of screening resultsEnhanced culture quality and efficiencyReal-time data acquisitionCompliant with FDA 21 CRF part 11Compatible with established protocolsSupports finding feeding pointsFacilitates optimization of harvesting strategiesThe Smart Measurement MethodThe sensor spots are fixed at the bottom of the shake flasks. Theluminescence of the dyes embedded in these sensor spots isexcited by the Shake Flask Reader. The luminescence lifetime isdetected non-invasively through the transparent flask. Theluminescence lifetime of the sensor dye depends on the oxygenpartial pressure and the pH of the sample, respectively. It isconverted to oxygen and pH values by the software using theprovided calibration data and internally stored conversion formula.?????????? ??SFR Shake Flask ReaderSystemsExamples for ApplicationsYeast & E. coli: Ensure Enough Oxygen SupplyS. cerevisiae grows on different sugars as carbon source. Whilegrowth on glucose and fructose is mainly fermentative, growth ongalactose is mainly respirative. This leads to low oxygenconcentration in the shake flasks. The accurately measured oxygenindicates the need to increase rotation frequency to avoid oxygenlimitation. High oxygen demand is typical for E. coli in its exponential phase. Inthe cultivation shown on the left, rotation speed had to be changedtwice in order to avoid oxygen limitation. In addition, changes in themetabolism can be detected by measuring DO. Schneider et al., University of Saarland, Saarbrücken, GermanyBioprocess Biosyst Eng., 33(5), 541 - 547, 2009Process Monitoring in Suspension-Adapted CHO Cell CulturesThe online measurement of dissolved oxygen concentration and pHin shaken bioreactors paves the way for proper scale down activitiesfrom bench-top stirred-tanks to smaller scales. Adjustment ofshaking speed as a function of pO2 is now possible avoiding possibleoxygen limitations at high cell densities. Even a simple pHreadjustment by tuning the pCO2 in the incubator is feasible tooptimize the output from simple experiments with shakenbioreactors.Dr. Robert Puskeiler, Roche Diagnostics, Penzberg, GermanySystematic Determination of Oxygen Uptake in Shake FlasksOxygen supply is crucial for almost all cultivations. In shake flasksfor a given geometry oxygen transfer is dependent on the shakingfrequency and the filling volume. The contour plot on the left showsoxygen transfer rates in 250 ml plastic shake flasks. By using asoftware for statistical design of experiments discrete volume androtation speed combinations were measured. As a result it ispossible to determine the kLa values for the typical working rangefor microbial and cell cultivations.Ries et al., ZHAW, Wädenswil, SwitzerlandEng. Life Sci 10, No. 1, 75 - 79, 2010?????????? ??????????????? ? ????? ????????? ???????????????????????????? ???? ??????"?# ?? $%? ?%? ?%&'&???() $%? ?%? ?%&'&?????? *?????? ??+?????? ?,??#???#?- ??? ????. /? ????)0??? 1???????? ??????????? ????????????????????SFR Shake Flask ReaderSystemsOxygen pH*SpecificationsMeasuring range 0 - 100 % O2 5.5 - 8.0 pHResponse time (t90) at 25 °C < 60 sec. < 60 sec.Resolution ± 0.01 % O2 at 0.21 % O2 ± 0.01 pH at pH = 7**± 0.1 % O2 at 20.9 % O2Accuracy ± 0.4 % O2 at 20.9 % O2 ± 0.1 pH at pH = 7 with one-point adjustment± 0.05 % O2 at 0.2 % O2 ± 0.2 pH at pH = 7 with pre-calibrationDrift < 0.01 % O2 per day (sampling interval of 1 min.) < 0.01 pH per day (sampling interval of 1 min.)PropertiesTemperature rangeCompatibilityCross-sensitivity Typically no cross-sensitivity in culture media Reduced to ionic strength (salinity); a high concentrationof small fluorescent molecules in the visible range can interfereStorage stability*provided Sensor Flasks are used without further handling in physiological solutions** at 100 rpm & cell culture mediafrom 5 °C to 50 °CAqueous solutions, ethanol (max. 10 % V/V), methanol (max. 10 % V/V), pH 2 - 10 12 months provided the sensor is stored in the darkSensor flasks are delivered irradiated.SY-SFR-11-01