Sphere Fluidics - Version ESI-Mine - Platform for High-Throughput, Miniaturised Electrospray Injection Mass Spectrometry (ESI-MS) Brochure

ESI-Mine™ can test up to 200,000 biomolecular samples (e.g. peptides, enzymes, antibodies, metabolites and small molecules) per day in miniaturized volumes of 500-700pL (picodroplets) facilitating more efficient screening and analysis in bioproduction or synthetic biology processes. Applying this novel approach, researchers can create a large library of picodroplets containing microorganisms producing proteins or small molecules of interest for analysis. One major drawback of using MS in analysis is that samples are usually destroyed and cannot be retrieved. To overcome this total sample destruction by ESI-MS, we have developed a unique microfluidics workflow where picodroplets are split into two replicate streams with identical clones, one stream is analyzed by ESI-MS, and the second stream is subsequently ‘held’ and only those ‘hit’ daughter picodroplets sorted based on the result of the MS analysis.