Synbiosis - Model ProtoCOL 3 - Brochure

Colony counting and zone measuringAutomated imagingCOLONY COUNTING &ZONE MEASURINGAn automated system for all your applicationsSynbiosis is the manufacturer and supplier of the world’s most popular automated colony counters and zone measurement systems.With over 25 years of experience, Synbiosis systems provide dedicated solutions for a wide range of applications. ProtoCOL3 isthe number one choice in the automated colony counting and zonemeasurement market.ProtoCOL 3 is the next generation instrument for colony counting, zone measuring (inhibition and AST), membranes, PETRIFILMTM plates and a rangeof other applications including: Spiral plates, OPKA, SBA, Multi-sector, Multi-well, SRD and Ames. ProtoCOL 3 enables plates of up to 150mm to be automatically read at the press of a button. Designed for use across a wide range of applications, ProtoCOL 3 will count colonies as small as 43 microns (0.043mm) or measure zones accurately to 0.5mmwith a theoretical detection limit of 0.1mm.ProtoCOL 3 has a unique LED lighting system configured for exceptionalillumination of all sample types.High definition, colour imagestaken with a 1.4 mega pixel scientific grade CCD camera ensure that even the smallest colony can be seen andcounted, while zone measurements are fast, accurate and reproducible. ADVANCED AUTOMATION &EFFICIENCY IN MICROBIOLOGYProtoCOL 3 is available with a mounted touch screen processor which enables ease of control while the easy-to-use software produces highly accurate results. Alternatively, ProtoCOL 3 can be used with a new or existing stand-alone desktop or laptop computer via USB connection - no additional PCI cards are required.ProtoCOL 3 comes inclusive with both colony counting and zone measuring ability. To extend its range of applications, inexpensive additional modules can be added to the software for Spiral, OPKA, SBA, Multi-sector, Multi-well, SRD and Ames as well as an optional bespoke statistics package. Results can be automatically transferred to Excel or a LIMS system. The system can also be used with 1D and 2D barcodes. All data generated is GLP/GMP compliant with a full audit trail and can be used to produce professional reports. Each system comes complete with 2 validation plates.PETRIFILMTM is a trademark of 3M Company. 3M Company is not affiliatedwith Synbiosis and does not endorse or certify Synbiosis’s products.Applications at the touch of a buttonPROTOCOL3 SOFTWAREProtoCOL 3 has twomethods of counting: Total Plate Count andColour Classification. Total plate count gives a quick and accuratecount. Colour classification allows colonies to be separated according tocolour, size and shape.Colonies grown on membrane filters can also be counted with ProtoCOL 3 using the grid removal function.Controls on the measure screen allow adjustments to be made on a plate by plate basis if required.Controls on the measure screen allow adjustments to be made on a plate by plate basis if required.• Modular design for application specific functionality• Displays only those commands relevant to each operation• Simple touch screen interface enhances productivity• Images and data automatically saved with each measurement• Installed SQL database facilitates LIMS connectivity• Results can easily be exported to spreadsheetsAt the heart of every ProtoCOL 3 colony counting and zone sizing system isthe innovative and intuitive ProtoCOL 3 software. Designed for maximum ease of use and with the needs of the busy microbiologist in mind, ProtoCOL 3software easily guides the user to the appropriate functions for each application. The great variety of modern microbiology techniques are easily addressed by the flexibility of ProtoCOL 3 software.PROTOCOL 3 - A SOLUTION FOR EVERY MICROBIOLOGYAPPLICATIONZones are measured at the click of a button to an accuracy of 0.5mm with a theoretical detection limit of 0.1mm.Edit function for count and zone measurements allowsplates to be adjusted if required, with a full audittrail to keep a track of any changes made.Customisable reportsproduced in Excel* orOpenOffice.*Excel licence requiredProcessorProtoCOL 3 Plus is supplied with a touch screen panel PC. ProtoCOL 3 requires connection to a stand-alone desktop orlaptop computer.DesignThe ergonomic design has a small footprint and looks good in any laboratory or production environment.CameraAn internal high resolution CCD camera captures high definition images and detects colonies as small as 0.043mm and zones to an accuracy of 0.5mm with a theoretical detection limit of 0.1mm.Sliding doorsTwo solid sliding doors can be positioned to eliminate external light.PlatformThe sample platform has interchangeable backgrounds (black, white, clear) for effective brightfield and darkfield exposure. Round plates from 55mm – 150mm can be used. Square plates up to 150 x 150mm can be viewed. For plates larger than this please contact us for further information.LightingA unique lighting configuration using red, green and blue long-life LEDs give outstanding colour imageswith excellent contrast.ProtoCOL 3 offers 4 times the resolution of similar systems.The innovative lighting system in ProtoCOL 3 exposes the plate to rapid bursts of red, green and blue light.The resulting colour composite image has excellent definition and clarity with no chromatic aberrations. An automated self calibration process ensures accurate colour definition with each exposure.Ease of use is the keyThe ergonomic design of ProtoCOL 3makes it very comfortable and easy to use.PROTOCOL3Applications you can image with a ProtoCOL 3PROTOCOL3 APPLICATIONSAmes testingThe Ames test is used to determine the degree of probable mutagenic activity likely to occur in the presenceof one or more chemicalsAntibiotic susceptibility test – ASTAntibiotic susceptibility is aterm used to describe thesensitivity of bacteria to anantibioticBioburden membranefiltrationA Bioburden test determinesthe approximate number of microorganisms on or in aproduct prior to sterilisationand acts as an early warningsystem for possible production problems which could lead to inadequate sterilisationEnvironmental monitoring – Settle platesEnvironmental monitoring describes the processes andactivities that need to takeplace to characterise andmonitor the quality of the environmentMicrobial limits test – membrane filtrationMicrobial Limits Test is designed to perform thequantitative estimations of specific viable microorganisms present in samples including membrane filtrationMicrobial limits testing –pour plateMicrobial Limits Test is designed to perform thequantitative estimations of specific viable microorganismspresent in samples. There arefour methods for this test ofwhich pour plate is the mostcommonMulti-sector platesMicrobiological monitoring ofthe air in facilities where pharmaceuticals and medicaldevices are produced is essential and well establishedOPKA – opsonophagocytickilling assayThe in vitro opsonophagocytickilling assay (OPKA) is essential for developing andimproving vaccines, particularly pneumococcalvaccines Preservative efficacy testingPreservative efficacy testing is required for the assessment of the antimicrobial preservation ofmultiple-use cosmetic andpharmaceutical productsSerum bactericidal assay – SBASerum bactericidal assay- SBA is a method ofmeasuring the bactericidal activity contained in a patient’s serum as a result ofantimicrobial therapySpiral platesSpiral plate is an apparatusfor the quantitative estimations of viable microorganisms present insamples. A logarithmically decreasing volume of sampleis spiraled on the surface of arotating Petri dishSingle radial immunodiffusion – SRDSRD is based on the diffusionof antigen from a circular well into a gel containing antiserum. A circle of precipitated antigen and antibody forms, and continuesto grow until equilibrium isreachedAPPLICATION SPECIFICSOFTWARE MODULESProtoCOL 3 is the perfect system for multiple applications in the food, water,clinical, pharmaceutical and health industries; the versatile ProtoCOL 3 optionsallow the user to customise the software to meet their application needs.Please refer to www.synbiosis.com for all ordering informationFEATURES AND BENEFITSHigh throughput capability suitable for production and large scale testing environmentsProtoCOL 3 is typically used when large numbers of plates need to be counted or measured rapidly and accurately. ProtoCOL 3 is therefore perfect for high throughput applications. All counting and measuringfunctions are automatic with results instantly sent to the internal reporting system.Colour imaging as standardThe unique LED lighting in the system gives full colour images. The colour classification feature of the ProtoCOL 3 software allows for an infinite range of colour options on the same plate. A choice of sample holder is included for both light and dark backgrounds, depending on sample type.Wide range of applications for versatilityThe system can be configured for a wide range of plate types. These include pour plates, spiral plates, multi-well,SBA, OPKA, Ames, inhibition zones, AST and SRD. Plates up to 150mm can be used on the standard unit, while an optional external scanning device can be added should larger plates need to be processed.Extensive report generation One of the major benefits of an automated system is that reports can be generated within the device. ProtoCOL 3 will produce reports that can easily be exported to Excel at the touch of a button.Traceability of dataAll the data generated has a full audit trail. All report generation is traceable and has an audit ‘lock’. Data is CFR 21 compliant and the system can be connected to a LIMS if required.High sensitivityProtoCOL 3 uses a highly sensitive CCD camera with a high resolution. Colonies as small as 43 microns (0.043mm) can be easily counted and zones can be accurately measured to 0.5mm with a theoretical detectionlimit of 0.1mm. HOW TO ORDERPROTOCOL3SPECIFICATIONS PROC3 PlusPROC3PROC3-SPIRAL-MODPROC3-OPKA-MODPROC3-AMES-MODPROC3-SECTOR-MODPROC3-SBA-MODPROC3-MWELL-MODProtoCOL3 PlusErgonomic housing constructed in highdensity foamIntegral CCD camera and lensMounted all-in-one PC2 sliding doors to prevent excessive ambient light1.4m pixel scientific grade CCD cameraUSB integral camera with f1.2 lensFor standard 150mm petri dish, smallestdetectable colony is 43 microns3 channel capture for colour imagesUnique 3 channel (red, green, blue) LED lighting (patent pending)Multi-array LED lighting (computer controlled)Lower lighting with upper reflectivelighting for all applicationsTouch screen panel PCColony counting and zone measurementsSeparation of touching colonies, exclusion areas, colour mode, shapemode, size modeWin7 compatibleSQL database stores all data and imagesProtoCOL3Ergonomic housing constructed in highdensity foamIntegral CCD camera and lens2 sliding doors to prevent excessive ambient light1.4m pixel scientific grade CCD cameraUSB integral camera with f1.2 lensFor standard 150mm petri dish, smallestdetectable colony is 43 microns3 channel capture for colour imagesUnique 3 channel (red, green, blue) LED lighting (patent pending)Multi-array LED lighting (computer controlled)Lower lighting with upper reflectivelighting for all applicationsUSBColony counting and zone measurementsSeparation of touching colonies, exclusion areas, colour mode, shapemode, size modeWin7 compatibleSQL database stores all data and imagesConstructionLight shieldCameraResolutionImagingLightingExternal connectionsMeasurement modesCount modesSoftwareDatabaseAll trademarks acknowledged A.0023.04.13ProtoCOL 3 automated colony counter and zone measuring system, with validation platesProtoCOL 3 is an ergonomically designed imaging unit with dark screens to eliminateambient light effects and improve imaging results. The unit contains a high resolution camera with red, blue and green LED lighting (patent pending) integrated to a processor and software. The software is accessed via a touch screen PC on themounted unit and features on-screen commands, allowing users to intuitively set their system up in minutes. These settings can be saved to make analysingthe same plate types at a later date, a quick one-touch process. Includes zone module for antibiotic susceptibility testing and inhibition zones and validation plates for counting and zone sizingAs above, without PCCounting software module for spiral plates, includes validation plateOPKA software module for opsonophagocytic killing assayAmes software module for the Bacterial Reverse Mutation TestMulti-sector plates software module for counting on multi-sector plates, eg, air sampleSBA software module for Serum Bactericidal Assay Module for counting muiti-well samplesSynbiosis Europe and International Headquarters: Beacon House Nuffield Road Cambridge CB4 1TF UKTel: +44 (0)1223 727125Fax: +44 (0)1223 727101 email: sales@synbiosis.comSynbiosis USA Headquarters:5108 Pegasus Court Suite L Frederick MD 21704 USATel: 800-686-4407/301-662-2863Fax: 301-631-3977 email: ussales@synbiosis.comWebsite: www.synbiosis.comA.0023.09.14All trademarks acknowledgedPlease refer to www.synbiosis.com for all ordering information